Making Custom Database and Sequence Alignment using NCBI BLAST+

A simple video tutorial to construct a custom protein/nucleotide database, sequence retrieval, and pairwise sequence alignment using NCBI BLAST+.
Download latest BLAST+ - ftp.ncbi.nlm.n...
Creating BLAST Protein Database
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./makeblastdb -in DB.fasta -out DB -dbtype prot -parse_seqids
Displaying List of Records in the Database
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./blastdbcmd -db DB -entry all -outfmt "OID: %o GI: %g ACC: %a IDENTIFIER: %i"
Displaying Contents in the Database
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./blastdbcmd -db DB -entry all
Searching Sequence from the Database
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./blastdbcmd -db DB -entry Sequence1
Retrieving Sequence from the Database
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./blastdbcmd -db DB -entry Sequence1 -out Sequence1.fasta
Performing Pairwise Alignment using BLAST+
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echo sequence | ./blastp -db DB
Saving the BLAST+ Pairwise Alignment Result
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echo sequence | ./blastp -db DB -outfmt 0 -out output.html -html
Identifiers:-
BLAST Ordinal Identifiers (BL_ORD_ID)
General (GNL)
Local (LCL)
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