Northern Blotting - Biology Tutorial

This is a tutorial on Northern Blotting. We cover some topics important for classes such as Biology, Biochemistry, Hitsology etc.
Steps of Northern Blotting:
- RNA Isolation;
- Separation of RNA's using Gel Electrophoresis;
- Blotting;
- Hybridization with Labelled Probe;
- Washing off excess probes;
- Visualization.
If you have any questions, don't be shy!! We hope we are able to clarify this topic.
Enjoy!
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[@user-yo6di5is9p]

Fantastic video. Very detailed, has all the key information and it's broken down simply :) Thank you.

[@JoaosLab]

Glad it was helpful! 🙌

[@steevensonbeaubrun6539]

With this piece of video, you just saved my Genetic Engineering exam. Good job!

[@kimtran9504]

Thanks so much! Super helpful for my test! :D

[@Nilay_Niloya]

Clear, practical and well detailed! Thanks a lot!

[@AppyGal19]

this video helped me soooo much! you used the correct terminology I was looking for. I appreciated the help!:)

[@andrealombarteserrat7621]

Great video, thank you so much for that, usually scientists have to learn all the tecnics that we must use for yourself, and this video helps a lot!

[@jamesyen98]

Thank you for uploading this video. It is very helpful to understand the concept.

[@jonqn07]

thank you VERY MUCH for your hard work!

[@akashjustforyou]

great piece of work..really appreciate....simple and up to mark...

[@apoorvasastry7073]

Loved it! Extremely well explained.

[@lenus8D]

thanks very much....finally understanding it!!!!! and it isn´t that complicated as it looked during the class :D

[@Blueberry31679]

Very helpful and clear! Thank you!

[@ignatiuswayiti2862]

U the man! i get every word through the video... keep doin what you doin..

[@JoaosLab]

Thank you! :) I'm glad you enjoyed it!

[@JoaosLab]

Glad to read that! We love their images too! :)

[@oyeman1000]

Loved it . Great video thanks

[@domenicoraddi6601]

This was really good. Keep it up!

[@Sharkbait173]

Great vid. Thanks!

[@promisendlovu8550]

lol....."dis is not a harmburger trust me" really enjoyed dis video.....thank you very much Sir !!!!!

[@nhunghoang2030]

Very useful. Thank you very much for the guide

[@molekulqrnabiologiq9912]

this video was very helpful,thanks :)

[@kevinraymondoluoch4319]

Great stuff!

[@gb546733]

wow! great tutorial

[@alfonsoortizcruz6116]

Great video! Thank you for the tutorial. God bless.

[@CACHATITAZITO]

Great video! very clear... I couldn't find the southern blot video. If you have the link, I'll be glad

[@magorzataprus163]

Really helpful I love it !

[@unicornsforreal]

Very easy to understand.

[@nevergone07]

thank you so much. it helpful for my seminar^^

[@Nina_SL]

You just saved my life!!

[@gemininga92]

amzing, very usefull for me and all people who speak english as the second language.

[@nicstandby]

Please, make a video about western blotting! Great video and thank you!

[@farmaciaufg878]

AMAIZING

[@Morinamora]

you saved me, thank you!

[@Pettyelettrodancer]

very clear!!! thanks!

[@laxballdmw]

Nice vid...well polished too.

[@ahmadfares5243]

Thanks, it's very helpful and clear

[@JoaosLab]

Glad it was helpful! 😊

[@robhitsemup]

Awesome!!!!!!

[@martinbakht2214]

Thank you!

[@JoaosLab]

Thinking about covering that topic soon! :)

[@questthefuture]

very great presentation, I appreciate the sense of humor :) , correct me if I am wrong, should the cDNA be a complementary sequence?

[@JoaosLab]

I believe the filter helps with the labeling treatment.

[@Fleshjesus]

Your video on southern blotting is offline. Can you please reupload it?

[@gabrielaferraz7963]

Muito bom o texto/desenhos e apresentação, fácil compreensão do ingles. Parabens!

[@k1morisato889]

nice job

[@maibar-chen6528]

Hi! great videos!
Where can I find the southern blot tutorial ?
Thanks!

[@pancreasdragonheart9765]

Does these filters/membranes has those labeled probes? Or what's the point with the filter?

[@sahay1madhulika]

Good explanation.

[@JoaosLab]

Glad it was helpful!

[@JoaosLab]

You are welcome!! :)

[@bismaazam5303]

in pcr we used primers that bind to the specefic complimentary dna sequences. but how can a cdna bind to rna . i dont understand

[@a.l.a.acunahamann2031]

Genio!! GRACIAS!!!!

[@ThuyPham-gf3ez]

Can we profile multiple RNA with this rechnique or it is only once at a time? Thanks

[@Nantidan]

is it possible to use SDS-PAGE instead of formaldehyd when openining the hairpin-structure of the RNA? (time 9:10)

[@afnankhalis1875]

a lot of your past videos, including the one about Southern blotting is no longer available, why?

[@Flynnbojangels]

What are the limits of detection of this experiment? Eg. sensitivity? What if you're trying to detect small siRNA or miRNA?

[@imagination7710]

Does formaldehyde just act as an acceptor of hydrogen bonds between base pairs?

[@BT-no3eh]

is there a video for western blotting that is this good?

[@JoaosLab]

You're welcome!

[@brunaantonini]

Which computer program is used to explain this technique?

[@nalindy254]

Excuses me !! This video explain about sample preparation of northern blot right ??

[@42marthacu]

how do you extract rna and not dna?

[@michaeltolkachov4749]

Cool, though usually amino benzyloxymethyl filter paper is used instead of nitrocellulose due to its higher affinity to RNA :)

[@uhRoid]

where is your southern blotting video?

[@yazminbrito1405]

why is the reason of the salt solution? :)

[@michaeltolkachov4749]

The salt (also buffer) solution allows an ionic transfer to occur. By capillary movement provided by the sponge with a weight pressure negatively charged RNAs transfer along with positively charged salt ions. RNA cannot pass through the amino benzyloxymethyl membrane and remains there, while other ions are absorbed by the filter paper.
I hope it'll help :)

[@yazminbrito1405]

Thank you!

[@vannguyen-gs3so]

wait arent u from kenhub?

[@unicornsforreal]

How would this work with siRNA?

[@unicornsforreal]

This question is for anyone who is an expert with this stuff.

[@aches6652]

why we use paper towels ?

[@kirkury]

Is Roger from American Dad narrating this?

[@JoaosLab]

Yes. 👽