Blue Native PAGE for Analysis of Respiratory Chain Supercomplexes | Protocol Preview

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Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes - a 2 minute Preview of the Experimental Protocol
Alexanne Cuillerier, Yan Burelle
University of Ottawa, Interdisciplinary School of Health Science, Faculty of Health Sciences and Department of Cellular and Molecular Medicine, Faculty of Medicine;
Here we present a protocol to extract, resolve and identify mitochondrial supercomplexes which minimizes exposure to detergents and Coomassie Blue. This protocol offers an optimal balance between resolution, and preservation of enzyme activities, while minimizing the risk of losing labile protein-protein interactions.
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