Build a Bioreactor for $10?

As part of a university project, I am working to develop a cost effective bioreactor which can be used to increase the ability of students to get hands-on experience with bioreactors. This first prototype is very rough and not completely working yet, but I want to document the build process to present a realistic prototyping workflow. Please let me know if you have any advice for future revisions!

[@edwardsolomonkwekugyimah4340]

Great work. Could you kindly provide the code and schematics

[@xlss8467]

Nice, i actually want to make a prototype for a bioreactor

[@justinschaeffer4364]

Could you use the air to turn your stirrer? Are you filtering your air going in?

[@snipes163]

Great info as always

[@fibranijevidra]

Why didn't you use msgnetic stirer? It can be made simpler, quicker and even cheaper with a computer cooler.

[@DIYBiotech]

You're absolutely right, I ended up doing this in future videos

[@mymobigoogle205]

Almost started to write the same comment. :)

[@justuskrekeler2142]

Is there any filtering of the air going on? Otherwise it might be a source of contamination or am I not seeing something?

[@DIYBiotech]

There was no air filtration in this prototype, but a simple sterile filter would fix this problem!

[@justuskrekeler2142]

@@DIYBiotech thanks mate, cool video btw! Leaving a sub!

[@DIYBiotech]

@@justuskrekeler2142 thanks!!

[@mymobigoogle205]

I think it must be autoclavebale (or how to say it in English) filter.

[@learningwithlowell]

This is genius.

[@nabuaaivan3731]

Great work! Can we have the materials that you use in your bioreactor? We really need it for our research.

[@medabya5295]

me too🥲😔

[@FantasticBiology]

Keep the hard work!

[@roytraz1371]

bro that's amazing

[@kathleenjoyceg.negapatan7631]

May I ask what materials you use on this bioreactor?? I need for research purposes.

[@Am-yo4hx]

how to contact you to make online meeting i need advices on bioreactor please thanks

[@luisrvizcaino]

Can I contact you? I want to do it in a bigger scale

[@AliensAreHoly]

Hey have you tried any growth test in yours yet?
A friend and I are busy with a similar project,
And not sure what the best culture would be to test as a functioning growth system, our current thoughts are a simple yeast culture.
Also ours is probably going to cost about $97

[@DIYBiotech]

Oh that's awesome! No I never tested a culture in it, but I think brewers yeast or kombucha culture would be good candidates as they have low risk of contamination

[@AliensAreHoly]

@@DIYBiotech yes I was thinking the same - but with no other equipment to actually quantify growth properly its tricky, I guess could take HD photos and use turbidity as a proxy - which is not science protocol, but might suffice for our purpose as we plan to carry non sterile/MMC fermentations.
We could also maybe obtain a culture which causes a colorometric change in the media used, which would be more scientifically sound, than going by turbidity :)

[@DIYBiotech]

@@AliensAreHoly you could also look into measuring density with a hydrometer like beer brewers use. Or use a refractometer. In my viking protein beer video I measured glucose production then decomposition with test strips

[@AliensAreHoly]

@@DIYBiotech aaah thanks for these solutions :)

[@AA-gl1dr]

@@AliensAreHoly you could always do a DIY nanodrop!

[@blar2112]

I like your MR2 toy

[@DIYBiotech]

Me too ☺️

[@writerbabe1]

Can you grow mammalian cells in this?

[@DIYBiotech]

Probably not... Mammalian cells are very susceptible to contamination and so more consideration should go into keeping things sterile

[@AA-gl1dr]

Instant sub

[@4ngling]

Jokes