Kurt Thorn (UCSF): Confocal Microscopy

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КОМЕНТАРІ • 73

  • @mrmatz408
    @mrmatz408 12 років тому +9

    Extremely thorough and well-prepared discussion of a topic in which I had no previous understanding. Totally great!

  • @nirmaljayadev
    @nirmaljayadev 9 років тому +12

    That's just extra ordinary talent. To make such a complicated thing so simple

  • @andrewareva4605
    @andrewareva4605 8 років тому +69

    If only this video was in higher resolution...

    • @scicommlab
      @scicommlab  8 років тому +11

      +Andrew Areva We re-recorded this video a couple of years ago with higher resolution. Check it (and a lot of other microscopy videos) here: ua-cam.com/video/1Q5V442V7xI/v-deo.html

    • @brandond8488
      @brandond8488 3 роки тому +1

      @@scicommlab Great use of super-resolution imaging 👀

    • @ErichAmMeer
      @ErichAmMeer 3 роки тому

      try confocal

  • @rob66181
    @rob66181 13 років тому +2

    Really nice video, thanks for uploading this. I suggest this to anyone who is going to use a confocal as its going to make your life a lot easier and will make it all a lot clearer. Thanks

  • @sylviahadai5291
    @sylviahadai5291 8 років тому +3

    Thank you so much for this excellent presentation!!! ❤

  • @xadroit
    @xadroit 13 років тому +1

    Thanks for an excellent, easy to follow introduction to confocal microscopy! That would have taken me hours to read:) Keep up the great work.

  • @mrunalsakharkar5463
    @mrunalsakharkar5463 7 років тому +1

    Excellent video. Very easy to follow. Thank you!

  • @scientia_potentia_est
    @scientia_potentia_est 6 років тому +1

    awesome vid, thanks to iBiology and Nikon! Wish the resolution was higher though.
    Crazy to think that after a few years (from the date of this video in 2010) laser scanning confocal has gotten so efficient with the new generation of insanely fast galvano-resonance hybrid scanners that spinning disk is getting phased out since you can get pretty much very close frame rates at comparable resolutions (something like 30 fps, 512x512) without the small compromise in optical sectioning that you get in spinning disk (which is not as bad as some people make it out to be though). Although 2-photon's still the boss, if you've got the $$$$$$ hehe

  • @MN-mj9ok
    @MN-mj9ok 3 роки тому +1

    Thank you for the very helpful video. I have one question.1. Why do we limit to 100µm imaging depth by confocal microscopy? Does imaging depth get better by decreasing pinhole size?

  • @hectorjosecardona
    @hectorjosecardona 6 років тому +1

    Excellent presentation, very didactic, thanks Kurt

  • @priye19
    @priye19 11 років тому +2

    Thank you. Very precise and informative.

  • @nareshbarik5384
    @nareshbarik5384 Місяць тому +1

    thank you

  • @anonviewerciv
    @anonviewerciv 4 роки тому

    13:45 Apparatus.
    18:28 Spinning disk.
    22:30 Multi-photon.

  • @charme2377
    @charme2377 6 років тому

    excellent video..i have a question please,i want to do a fluorescent exerience with confical and 1 EMCCD camera ..what is the difference between using 2 camera or 2 ...thank you

  • @maxencehannard1988
    @maxencehannard1988 3 роки тому

    Thanks for this wonderful video

  • @MohammadAlKobaisi
    @MohammadAlKobaisi 10 років тому +3

    Very helpful, thanks!

  • @djalitanaful
    @djalitanaful 8 років тому

    I am stuck, can't get it. trying hard to understand how to see molecular interactions using fluorescence and confocal microscope. is there any possibility to do a video before this and explain basics of how light interacts with specimen?

  • @psamrai7
    @psamrai7 11 років тому

    is there a difference between the dichromatic mirror and dichrioic mirror?

  • @antoniodimaggio4779
    @antoniodimaggio4779 4 роки тому +1

    this is such a good one! Thank you so much

  • @sylviahadai5291
    @sylviahadai5291 8 років тому +2

    Thank you so much!!

  • @caminhodeperfeicao273
    @caminhodeperfeicao273 3 роки тому

    Thank you is very much for the video!

  • @amarmirza08
    @amarmirza08 12 років тому

    Thank you for making this video.

  • @megatog2011
    @megatog2011 12 років тому

    Very nice presentation there. However, if it was in HD (720p or better), I would have seen and maybe understood more ;)

  • @mindlessgreen
    @mindlessgreen 13 років тому

    Fantastic. Very well explained.

  • @KeyManiacLad
    @KeyManiacLad 11 років тому

    Excellent presentation!

  • @Nanomachines5on
    @Nanomachines5on 11 років тому

    Good explanation of the diagrams. Thank you!

  • @sorrzietgiest
    @sorrzietgiest 11 років тому

    wait.. working of spiral spinning disc is not very clear..

  • @aman0311tisf
    @aman0311tisf 13 років тому

    Excellent work Kurt!!! I've addad a link on my blog -

  • @paolofagherazzi1684
    @paolofagherazzi1684 6 років тому

    Thank You very much

  • @BushCampingTools
    @BushCampingTools 8 років тому +1

    Confocal and multi-photon way to go! Spent years in research using, and later selling LOL!

  • @djinnahwell
    @djinnahwell 14 років тому

    Thanks, Thanks ! So great video.

  • @garrystewart3836
    @garrystewart3836 10 років тому

    Great help. Thanks!

  • @painlessvoodoochild
    @painlessvoodoochild 14 років тому

    Thanks for great explanation

  • @redpinkgreenandblue
    @redpinkgreenandblue 10 років тому +1

    very helpful, thank you :)

  • @ahmadnabawi5060
    @ahmadnabawi5060 10 років тому

    a life saver !

  • @Maryam85ful
    @Maryam85ful 12 років тому

    Very nice video! thanks for uploading :)

  • @dolet8
    @dolet8 12 років тому

    explained very clear.

  • @9haylee
    @9haylee 10 років тому

    Excellent!

  • @peterhartman55
    @peterhartman55 11 років тому

    Great video.

  • @VSerman13GR
    @VSerman13GR 10 років тому

    Thank you!

  • @edwindeleon1130
    @edwindeleon1130 5 років тому

    need elctron microscope for hiv diagnoses

  • @FryChicken
    @FryChicken 13 років тому

    Confocal Microscopy starts at 3:50

  • @leixiao169
    @leixiao169 7 років тому

    fantastic!!

  • @sorrzietgiest
    @sorrzietgiest 11 років тому

    very clear... helped me a lot... thanks..:)

  • @hitgucvkj
    @hitgucvkj 14 років тому

    Awesome!

  • @uvesca
    @uvesca 14 років тому

    yes... Awesome!

  • @sorrzietgiest
    @sorrzietgiest 11 років тому

    spinning spiral mirror working is not very clear

  • @zhangcanding3263
    @zhangcanding3263 7 років тому

    Great! Thanks

  • @gregrobertson9732
    @gregrobertson9732 9 років тому

    Nice one!

  • @sasamaster
    @sasamaster 13 років тому

    Excelente! Muchas gracias, esto me ayudó mucho. :-)

  • @xplorer9
    @xplorer9 13 років тому

    awesome!

  • @xtremetom180
    @xtremetom180 13 років тому

    very interesting thanks

  • @doktonic1370
    @doktonic1370 4 роки тому

    hell yes

  • @FatemahRezayee
    @FatemahRezayee 10 років тому

    Thanks a lot

  • @humbertosmith6985
    @humbertosmith6985 2 роки тому +1

    Opino. Que. Diyegnnoñojia. Es. Maravillosa. Pero. Soy. Una. Simple. S🎃😊😅😂😂

  • @q0L0b
    @q0L0b 14 років тому

    LIKE.

  • @mmartin5816
    @mmartin5816 5 років тому

    If only microscopists and histology people added scale bars. Don't show me an image without context!

  • @kevinggyy
    @kevinggyy 13 років тому

    like

  • @lotharmayring6063
    @lotharmayring6063 Рік тому

    unsharp unsharp unsharp unsharp unsharp ..................................

  • @preynoldssco
    @preynoldssco 9 років тому

    Why is this potato quality?

    • @darlantro
      @darlantro 9 років тому

      uploaded 5+ years ago. UA-cam had harsher compression requirements back then.

    • @darlantro
      @darlantro 9 років тому

      also, probably an old camera

  • @venividiviolini6459
    @venividiviolini6459 6 років тому

    "you can see that there is stuff that is clear and sharp and in focus.." yeah.. nah not really :'D

  • @sylviahadai5291
    @sylviahadai5291 8 років тому +2

    Thank you so much for this excellent presentation!!! ❤

  • @sylviahadai5291
    @sylviahadai5291 8 років тому +2

    Thank you so much!!

  • @lizs7827
    @lizs7827 6 років тому

    Very helpful, thanks!

  • @xplorer9
    @xplorer9 13 років тому

    Awesome!

  • @vman049
    @vman049 12 років тому

    Awesome!