Glad you're back! Missed the quality of your vides. So, as you know, since 2005 the Conboys have moved on to focusing on old plasma (2020 and 2022 papers) as a major contributor to aging and shown that removal of a large fraction of plasma is rejuvenating, although for how long is not known. I'm wondering, with EVs being discussed, if one mechanism of plasma replacement is the accelerated turnover of EVs. Plasma replacement does show increased expression of youthful genes. Plasma replacement has the advantage of being free to do on smaller scale at local blood bank and can be done instead of normal whole blood donation (25% plasma volume per donation).
@@blainebowling3303 I wasn't clear, just have to ask to do plasma donation instead of the normal whole blood donation. Even platelet donation will remove a good fraction of plasma and can be done frequently. They can tell you how much plasma you loose each donation as they have to track in the US.
There is a very recent interview with Dr. Harald Katcher at peak human labs. It's only a couple of weeks old. It's worth watching if, like myself, you find Dr. Harold's work is interesting.
This has been covered for the last two years. Though has been given little attention in the major media, simply because of the manner in which it has been developed more or less on a shoestring. This is a further development of the procedure that was first reported long ago, perhaps in the fifties, if my memory serves, wherein the circulatory systems of a young and old mouse where tied together. Which rejuvenated the old rat. There are clinics all over the world where the wealthy get plasma transfusions from young people who are paid large sums for pints of their blood.
Glad to see you back again. But my question is about how can be peer reviewed a study like Katcher's having E5 - its core compound with their effect on aging supossed to be replicated by peers - under the secrecy of a patent?.
Good to see you are making more videos, I love how dense they are, sometimes it takes me a few watches to fully understand everything you're saying. I run BOINC to contribute to health research and would love to see you talk about the results of a BOINC project one day. None are currently focused on longevity, but there are a number of health research ones like World Community Grid (mostly cancer) and SiDock (covid).
Are you going to make another video on this? So yeah basically they isolated exosomes from pig plasma. Though just so you know exosomes are usually typically collected from mesenchymal stem cells.
Is there anything in particular why pigs are selected? I also read about pig tissue used in lab-grown organ studies... seems like regeneration research often involves pigs
Because they are readily available and give a decent amount of blood. They also tried with cows, I think, but they couldn't get high quality E5 from it for some reason.
Cows might be more ideal, but they did the study in India and doing stuff to cows there is like trying to do stuff to someone's grandmother. It's possible any animal from fish on up might work, but it'd probably be better to use something closer to us. Pigs are among the most slaughtered mammals we have around.
"Blood-thirsty for more content"- ha! Regarding, micro RNA as the the active agent ... would micro RNS work across different types of mammals? Looking at Dr. Katcher's experiment with E5 on his hands, it appears that the rejuvenation of his skin is related to stem cells. Otherwise, how could one explain that the rejuvenated skin is aging at a normal rate? That is, there has to be something in EVs that is promoting stem cell generation.
Make you a deal: first let's stop all the other animals in nature killing each other, then us humans can do it too. One animal killing another for survival isn't "greed and vanity". It's just the way things have been for millions of years.
I understand your concern, but please note, it is not necessary to either kill or exploit animals in order to do this. Yes, blood that was just thrown away at slaughter houses or dried and used as fertilizer, will probably be used, BUT that is not really necessary. And if the same proportion of the animal blood is taken as is when a human donates blood to the red cross, it will actually increase the animals lifespan, which would be possible without causing pain or trauma. If interested you could do a little research and you will find out that people who regularly give blood live longer than those who do not. So instead of making a kneejerk comment about exploiting animals, you could be a voice for using the same animals many times during their youth. Needless to say, it will be very difficult to convince people who consume animals to care if a waste product of the unfortunate animals that are slaughtered for food was used to rejuvenate humans.
That's the grip strength test where the treated rats use four paws and the untreated used two. It's all bull crap if the experimenters know which rats they are testing. Double blind placebo controled or it's trash.
True, this is mostly a result of the lack of funding. You may find the fact that with one topical application, Katchner was able to rejuvenate one of his hands. The skin became thicker and more supple, and the age spots disappeared. Kind of amazing when he holds them next to one another.
@@kx4532 They apparently developed a type of topical salve from the plasma in a lab in India, and Katchner got to treat one of his hand with it. So I guess you could call it that. See for yourself. ua-cam.com/video/PAX606_Esrs/v-deo.html
@@toby-xo6rb No, apparently they also did a memory and discovery test with the rats too, along with may tests of the organs and blood of treated rats. They were very limited in funds and this research was done at a college in India that was primarily a business school.
Nope, that wouldn't work, just give you an unset stomach, but that Countess who used to torture young girls then bath in their blood was on the right track. In a way.
I'm currently pursuing a mastery's degree. Having recently come out of college, I wanted to thank you for your videos. They have brought me a lot of insight into the world of science and how to interpret these results and graphs, particularly in the aging field of study which is what I'd like to do for a living. But also, for informing people about all these recent discoveries which are very exciting, keep doing what you do :)
Hopefully this will lead to new areas of research, and eventually practical methods for improving human health, youth, and longevity. I am glad that you are still here keeping us up to date on new research, and this video is great as always.
I've wondered if you could get a young person to donate blood of the same type and infuse the plasma for youthful benefits. This suggests that might be possible with piglet blood. I love you accent and you explain biochemistry so well. I had to memorize Kreb's cycle in one of my biochem classes. Have forgotten it since.
What you are suggesting has been going on at clinics worldwide for years. Katchners approach could make it available to everyone on earth, perhaps for free, instead of the super-weathly, whom themselves are being exploited by these clinics. My understanding is that it would be possible to use piglet blood, or the blood of horses, or sheep, or virtually any mammal.
@@clown6507 This work was stimulated by a fever Dream that Katchner had in his youth, where he was alive hundreds of years in the future and being celebrated for having brought immortality to mankind.
Apparently all young mammals, they used pigs because they are eaten by some people in India while cows are considered sacred. This research took place in India.
The larger the animal, the greater the quantity, I'd imagine. I'd be more concerned about purity, especially considering what gets on at the factory farms that produce cheap meat.
Great to see Dr Harold's work being talked about. I think its the most promising work in this field by a country mile. Why not try get an interview with the good Doctor Himself? He is on linked in. A very agreeable guy. I'm sure he would enjoy discussing his work with you.
There are medicines on the market with a pig brain derived neurotrophic growth factors, called Cerebrolysin. I wonder if you also could increase the absorption of these peptides when you encapsulate those with a PEG molecule.
I hope Harold is successful in this. Not just for the good of humanity, but because major pharmaceutical companies have spent hundreds of millions of dollars attempting to do this without success.
We would never be able to afford to do this at scale with the tens of millions or hundreds of millions of elderly, even if nearly all the youth were tapped. And synthesizing this sounds near impossible. Maybe a couple hundred million very genetically modified pigs could bring down the cost somewhat, but it would still be high, and that would just be the production cost, before the usual big pharma gouging. And don't kid yourself, they will get involved, if this works. No one can afford all the testing required, and scientists always take the tens to hundreds of millions offered.
This therapy would likely be very expensive if derived from plasma donors. And synthesizing it is probably beyond our current technology. I guess this should not be a surprise. What I'd like to see is if injections of albumin can extend life. Recently, a higher level was shown to be an indicator of survival in extreme longevity when studying mostly centenarians. Albumin is a lot easier, and cheaper, to make. If we kept it at youthful levels in mice, well, I would like to see what would happen.
This would be for everyone, not just the silicon valley millionaires who can afford plasma transfusions from their young blood boys as has been happening. Which procedure is not entirely without risk.
He’s not using it on himself. I don’t know why not. He’s 78 and looks and sounds 88. What’s the worst that could happen? He could…die? If he used it on himself now, and it worked, he would have no shortage of money to do whatever he wanted. That’s one reason why all of this seems kind of sketchy to me. What the hell is he waiting for?
@@unutilisateur4729He does have enough to use it on himself. They’re about to do a large dog study, so they must have enough of it. So why is he waiting? The guy could literally drop dead tomorrow. The mice he used it on had no bad side effects. Weird.
@@squamish4244 They don't have enough to do a human trial, Katcher himself said so multiple times. Beagles aren't large dogs. And even if they had enough for one person, you usually want to include multiple participants in your study
We have missed you.
Glad you're back! Missed the quality of your vides. So, as you know, since 2005 the Conboys have moved on to focusing on old plasma (2020 and 2022 papers) as a major contributor to aging and shown that removal of a large fraction of plasma is rejuvenating, although for how long is not known. I'm wondering, with EVs being discussed, if one mechanism of plasma replacement is the accelerated turnover of EVs. Plasma replacement does show increased expression of youthful genes. Plasma replacement has the advantage of being free to do on smaller scale at local blood bank and can be done instead of normal whole blood donation (25% plasma volume per donation).
I give a Power Red donation a few times a year and was wondering about that exact thing…
@@blainebowling3303 I wasn't clear, just have to ask to do plasma donation instead of the normal whole blood donation. Even platelet donation will remove a good fraction of plasma and can be done frequently. They can tell you how much plasma you loose each donation as they have to track in the US.
welcome back!
Glad you're back!
You are a wonderful contributor and communicator. I and the community really enjoy your clear precise and helpful videos. Thank you.
Good to see someone besides modern healthspan is covering this. I have been waiting years for further info about this.
There is a very recent interview with Dr. Harald Katcher at peak human labs. It's only a couple of weeks old. It's worth watching if, like myself, you find Dr. Harold's work is interesting.
@@anthonymason385 ua-cam.com/video/4kydtgZeXec/v-deo.html
Welcome back, Eleanor! We kept the light on for ya. 🙂
Welcome back! Missed your vids.
Great to see my favorite science reporter back in the grind again.
Amazing how the "real media" never covers anything this important. Guess I should contribute to the Patreon here ;)
This has been covered for the last two years. Though has been given little attention in the major media, simply because of the manner in which it has been developed more or less on a shoestring. This is a further development of the procedure that was first reported long ago, perhaps in the fifties, if my memory serves, wherein the circulatory systems of a young and old mouse where tied together. Which rejuvenated the old rat. There are clinics all over the world where the wealthy get plasma transfusions from young people who are paid large sums for pints of their blood.
Glad to see you back again. But my question is about how can be peer reviewed a study like Katcher's having E5 - its core compound with their effect on aging supossed to be replicated by peers - under the secrecy of a patent?.
The patent means they can finally release this information and do more studies. It is available to read online.
Good to see you are making more videos, I love how dense they are, sometimes it takes me a few watches to fully understand everything you're saying. I run BOINC to contribute to health research and would love to see you talk about the results of a BOINC project one day. None are currently focused on longevity, but there are a number of health research ones like World Community Grid (mostly cancer) and SiDock (covid).
Are you going to make another video on this? So yeah basically they isolated exosomes from pig plasma. Though just so you know exosomes are usually typically collected from mesenchymal stem cells.
Is there anything in particular why pigs are selected?
I also read about pig tissue used in lab-grown organ studies... seems like regeneration research often involves pigs
Because they are readily available and give a decent amount of blood. They also tried with cows, I think, but they couldn't get high quality E5 from it for some reason.
@@unutilisateur4729 what about sheep?
Cows might be more ideal, but they did the study in India and doing stuff to cows there is like trying to do stuff to someone's grandmother.
It's possible any animal from fish on up might work, but it'd probably be better to use something closer to us. Pigs are among the most slaughtered mammals we have around.
E stands for elixir.
"Blood-thirsty for more content"- ha!
Regarding, micro RNA as the the active agent ... would micro RNS work across different types of mammals?
Looking at Dr. Katcher's experiment with E5 on his hands, it appears that the rejuvenation of his skin is related to stem cells. Otherwise, how could one explain that the rejuvenated skin is aging at a normal rate? That is, there has to be something in EVs that is promoting stem cell generation.
Wonderful science which offers a lots of possibilities and opportunities for the betterment of the human species!
In +14 I could ask My daughter to donate plasma to keep me healthy?
Can we just not exploit and kill animals for our greed and vanity, for once!?
The blood is a byproduct and is currently wasted. This would be using more of the animal that is already killed for sausages
It might be possible to scale up cloning the cells that make these ECVs, and make E5 without needing whole animals.
Make you a deal: first let's stop all the other animals in nature killing each other, then us humans can do it too. One animal killing another for survival isn't "greed and vanity". It's just the way things have been for millions of years.
Survival is greed and vanity?
I understand your concern, but please note, it is not necessary to either kill or exploit animals in order to do this. Yes, blood that was just thrown away at slaughter houses or dried and used as fertilizer, will probably be used, BUT that is not really necessary. And if the same proportion of the animal blood is taken as is when a human donates blood to the red cross, it will actually increase the animals lifespan, which would be possible without causing pain or trauma. If interested you could do a little research and you will find out that people who regularly give blood live longer than those who do not. So instead of making a kneejerk comment about exploiting animals, you could be a voice for using the same animals many times during their youth.
Needless to say, it will be very difficult to convince people who consume animals to care if a waste product of the unfortunate animals that are slaughtered for food was used to rejuvenate humans.
That's the grip strength test where the treated rats use four paws and the untreated used two. It's all bull crap if the experimenters know which rats they are testing. Double blind placebo controled or it's trash.
So you're saying the grip strength test was the ONLY test they did?
True, this is mostly a result of the lack of funding. You may find the fact that with one topical application, Katchner was able to rejuvenate one of his hands. The skin became thicker and more supple, and the age spots disappeared. Kind of amazing when he holds them next to one another.
@@SkyRiver1 Topical blood plasma :(
@@kx4532 They apparently developed a type of topical salve from the plasma in a lab in India, and Katchner got to treat one of his hand with it. So I guess you could call it that. See for yourself. ua-cam.com/video/PAX606_Esrs/v-deo.html
@@toby-xo6rb No, apparently they also did a memory and discovery test with the rats too, along with may tests of the organs and blood of treated rats. They were very limited in funds and this research was done at a college in India that was primarily a business school.
Vampires were right! 😱
Nope, that wouldn't work, just give you an unset stomach, but that Countess who used to torture young girls then bath in their blood was on the right track. In a way.
I'm currently pursuing a mastery's degree. Having recently come out of college, I wanted to thank you for your videos. They have brought me a lot of insight into the world of science and how to interpret these results and graphs, particularly in the aging field of study which is what I'd like to do for a living.
But also, for informing people about all these recent discoveries which are very exciting, keep doing what you do :)
Hopefully this will lead to new areas of research, and eventually practical methods for improving human health, youth, and longevity. I am glad that you are still here keeping us up to date on new research, and this video is great as always.
I love the accent and intellect in your voice.
Oh! E5, E xtracelluar V esicles (V for 5)
Now, I really hope so
It makes sense.. :-)
I've wondered if you could get a young person to donate blood of the same type and infuse the plasma for youthful benefits. This suggests that might be possible with piglet blood. I love you accent and you explain biochemistry so well. I had to memorize Kreb's cycle in one of my biochem classes. Have forgotten it since.
What you are suggesting has been going on at clinics worldwide for years. Katchners approach could make it available to everyone on earth, perhaps for free, instead of the super-weathly, whom themselves are being exploited by these clinics. My understanding is that it would be possible to use piglet blood, or the blood of horses, or sheep, or virtually any mammal.
Please tell me this is real.
Im older than ive ever been
And now I'm even older
Its real like reincarnation lol
@@clown6507 This work was stimulated by a fever Dream that Katchner had in his youth, where he was alive hundreds of years in the future and being celebrated for having brought immortality to mankind.
@@clown6507So…it is real.
Thank you for your breakdown! It is a very encouraging development. Are there techniques to sequence the mRNAs in ecms?
Can E5 only be found on youthful pigs? do young lab mice produce it?
Apparently all young mammals, they used pigs because they are eaten by some people in India while cows are considered sacred.
This research took place in India.
According to the patent, you can get it from just about any mammal.
The larger the animal, the greater the quantity, I'd imagine.
I'd be more concerned about purity, especially considering what gets on at the factory farms that produce cheap meat.
you would need about 20-24 liters of blood for a human. How many lab mice is that?
Great to see Dr Harold's work being talked about. I think its the most promising work in this field by a country mile.
Why not try get an interview with the good Doctor Himself? He is on linked in. A very agreeable guy. I'm sure he would enjoy discussing his work with you.
There are medicines on the market with a pig brain derived neurotrophic growth factors, called Cerebrolysin. I wonder if you also could increase the absorption of these peptides when you encapsulate those with a PEG molecule.
Sounds like you're getting some of the manerisms of Nick from Physionc. Not sure it works, but I'm a big fan of both of you anyways.
I hope Harold is successful in this. Not just for the good of humanity, but because major pharmaceutical companies have spent hundreds of millions of dollars attempting to do this without success.
We would never be able to afford to do this at scale with the tens of millions or hundreds of millions of elderly, even if nearly all the youth were tapped. And synthesizing this sounds near impossible. Maybe a couple hundred million very genetically modified pigs could bring down the cost somewhat, but it would still be high, and that would just be the production cost, before the usual big pharma gouging. And don't kid yourself, they will get involved, if this works. No one can afford all the testing required, and scientists always take the tens to hundreds of millions offered.
I wonder why they have failed.
This therapy would likely be very expensive if derived from plasma donors. And synthesizing it is probably beyond our current technology.
I guess this should not be a surprise.
What I'd like to see is if injections of albumin can extend life. Recently, a higher level was shown to be an indicator of survival in extreme longevity when studying mostly centenarians. Albumin is a lot easier, and cheaper, to make. If we kept it at youthful levels in mice, well, I would like to see what would happen.
I would guess not!
This would be for everyone, not just the silicon valley millionaires who can afford plasma transfusions from their young blood boys as has been happening. Which procedure is not entirely without risk.
It doesn’t seem to work, either. At least not the way they are doing it.
This sounds similar to Actovegin which is calf blood hemodialysate combined with exosomes.
Barely recognized Harold. Whatever he's working on, doesn't look very promising if he's taking it himself. Geezus
He has tried it on one of his hands. I guess he'll be the first to use it when they have enough to treat people instead of small animals.
He’s not using it on himself. I don’t know why not. He’s 78 and looks and sounds 88. What’s the worst that could happen? He could…die?
If he used it on himself now, and it worked, he would have no shortage of money to do whatever he wanted. That’s one reason why all of this seems kind of sketchy to me. What the hell is he waiting for?
@@unutilisateur4729He does have enough to use it on himself. They’re about to do a large dog study, so they must have enough of it. So why is he waiting? The guy could literally drop dead tomorrow. The mice he used it on had no bad side effects. Weird.
@@squamish4244 They don't have enough to do a human trial, Katcher himself said so multiple times. Beagles aren't large dogs. And even if they had enough for one person, you usually want to include multiple participants in your study