Translation Initiation in Eukaryotes

Eukaryotic translation is the biological process by which messenger RNA is translated into proteins in eukaryotes. It consists of four phases: initiation, elongation, termination, and recycling.
CAP-DEPENDENT AND CAP INDEPENDENT INITIATION PATHWAYS :
Initiation of translation usually involves the interaction of certain key proteins, the initiation factors, with a special tag bound to the 5'-end of an mRNA molecule, the 5' cap, as well as with the 5' UTR. These proteins bind the small (40S) ribosomal subunit and hold the mRNA in place. eIF3 is associated with the 40S ribosomal subunit and plays a role in keeping the large (60S) ribosomal subunit from prematurely binding. eIF3 also interacts with the eIF4F complex, which consists of three other initiation factors: eIF4A, eIF4E, and eIF4G. eIF4G is a scaffolding protein that directly associates with both eIF3 and the other two components.
The best-studied example of cap-independent translation initiation in eukaryotes uses the internal ribosome entry site (IRES). Unlike cap-dependent translation, cap-independent translation does not require a 5' cap to initiate scanning from the 5' end of the mRNA until the start codon.

[@AarushiK.P]

It's 3am right now, I am pulling an all-nighter and feel so relieved to have FINALLY understood all the eIAFs in this mechanism. Thank you so much.

[@hussainbiology]

most welcome...

[@izzanare2605]

i have my molecular biology exam in three days thanks for explaining this better than my professor

[@hussainbiology]

thanks Liza for appreciation... Really Glad to know that it helps......
All the best ahead

[@malthekjrbendtsen194]

Me too, good luck! :D

[@hussainbiology]

@@malthekjrbendtsen194 ❤️ All the best

[@huzaifa2k1]

On 5th of January 2023 I'm having my final exam for molecular Biology (I'm doing Bsc in Biotechnology and Genetic Engineering currently 5th semester final exam gonna start).
And this guy is really really something else.. I haven't understood from my teacher/professor but these lectures are so so so so well explained and easy to understand... A 100% recommended thing... Keep it up
Pray for Me, 🤲

[@hussainbiology]

thanks for appreciation.....and U will rock in exam... i am sure.... All the best

[@huzaifa2k1]

@@hussainbiology Thank you for the motivation.... Jazakallah ❤️💜

[@sreejabhattacharya4511]

​@@huzaifa2k1Even I am doing B. Tech in Biotechnology and I don't understand a thing my professor teaches 😭.

[@achalrathore5564]

U are the best teacher for molecular biology.... Very easily u clear such tough concepts... Thankyou so much for all the topics u teach but please upload the vedio for elongation and termination process..
Your method make these topics very interesting so please sir complete this eukaryotic translation
Thank you so much

[@hussainbiology]

those videos are already available .. plz check the playlist

[@surayajan190]

Thank you very much sir In a few minutes u have explained it so well

[@hussainbiology]

You are most welcome

[@snehavraj6375]

thanks alot ....may god bless you

[@hussainbiology]

God bless u too

[@misshaikh_14]

Amazing explanation...you are great 👍 👌

[@hussainbiology]

Thanks a lot 😊

[@aditishinde3022]

Ur teaching is the best

[@hussainbiology]

thanks Aditi for appreciation..Glad it helps

[@ammarahchaudhary2820]

Sir plzzz upload next videos as early as possible plzzzzzzzz don't take long time ,
Plzzzzzzz upload two lectures in one week.

[@ridhimagaur1213]

very nice video in 6 minutes you have taught us so much that we were not able to understand in 1 hour class, what an amazing teacher

[@anxiousaquarius6926]

Thank you for explaining such a complicated process in detail!

[@hussainbiology]

thanks for appreciation.. Glad to know that it helps ✌️

[@ankitayadav1123]

Ofcourse as usual awesome explanation 😀 thank you so much
And waiting for further vedios 🤗

[@hussainbiology]

thanks Anamika for appreciation...Glad to know that it helps ✌️

[@ajeshjoshi3364]

Well explain better than other

[@hussainbiology]

thanks for appreciation..Glad it helps....✌️

[@fuzailahmad00786]

Jazak Allah khair 🤲

[@hussainbiology]

✌️

[@sumitmaity5312]

How easily explain...nice class

[@hussainbiology]

thanks for appreciation

[@Scholarship_hunters]

YOU ARE DOING GREAT SIR

[@tanu6464]

😊😊😊😊😊thanku

[@izrai911]

So very helpful!!! Thank you so much 😆

[@ammarahchaudhary2820]

What is circulization of mRNA why we use it ,and what is their function and plzzzzz also told kozak sequence also

[@Ahmadkhan-bq2hc]

Mashaallah sir
Ultimate 👍

[@hussainbiology]

Thanks

[@rahulreddy449]

Thanks for this easy explanation 😊

[@hussainbiology]

My pleasure 😊

[@avinavsingh4463]

This video was good although I have some doubts
1. Where is eIF2B, eIF5 used??? (As it's binding and animation is not mentioned in this video)
2. can you please be more elaborate with eIF5 and eIF5B functioning. As in the previous video you've mentioned eIF5B dissociates all the factors from 80SrRNA (which acc. to me probably must occur at the ending step of initiation)but here in this video eIF5B attaches so early in the 40SrRNA.

[@debaswapnaray3295]

Thanx..it's really helpful for all students...👌

[@hussainbiology]

thanks for appreciation..Glad to know that it helps ✌️

[@biologicalresearch5836]

Do u know prof. ashish sharma sir (ex faculty of GB), your clearity of concepts make me feel like you are his student.

[@hussainbiology]

Thanks for appreciation... But i ain't a student of Ashish Sir... i wish i too had great teacher's like u said of Ashish Sir....✌️✌️

[@arpitmishra5532]

Very nice sir

[@hussainbiology]

Keep watching

[@HectixShorts]

This is a great description. Thanks

[@hussainbiology]

thanks for appreciation

[@ammaramehak9473]

AOa sir , sir you read transcription if you read transcription where is videos of transcription elongation and termination in
eukaryote plzzzzzzzzzzzz told and upload that videos also my exams are very near it,s humble request to upload transcription videos and next video early on you channel.
Be happly doing great work .

[@tristankoops2134]

Bester Mann

[@anitaprajapat8480]

Thanks sir for giving help 🙏🙏

[@hussainbiology]

It's my pleasure

[@sanjaykumar-tj6cg]

Best sir

[@ammarahmehak9986]

plzzz upload next video

[@alirezahekmati7632]

gold!

[@aditishinde3022]

Sir nice video

[@hussainbiology]

✌️

[@ammarahmehak9986]

plz told whole termination I read watson but I can't understand their words

[@hussainbiology]

okay.

[@ammarahchaudhary2820]

Sir plzzzzzzzzz give your email I'd
and that my e mail I'd is [e-mail] for answers of following questions that asked few hrs before.

[@ammarahmehak9986]

model of transcription ,transient mode,l Inchworming model, and Scrunching model, function of ctd tail why it required.

[@aditishinde3022]

Big fan sir

[@hussainbiology]

So glad to know that ... Really appreciate your support ❤️

[@thanglam7567]

Could u make the subtitle available please? Thanks

[@pratibhamaurya3196]

Thankyou so much sir 😊🙏🇮🇳

[@hussainbiology]

you are welcome

[@umaasaraf3876]

Sir your videos are very helpful.It gives clear understanding of the topic Can you please make a video on elongation and termination of translation ?

[@hussainbiology]

first of all thanks for appreciation...
aur shyd maine Woh Videos banaye hai...

[@getitdone0010]

amazing thank you

[@hussainbiology]

thanks Maryam for appreciation..Glad it helps ✌️

[@ammaramehak9473]

plzzzzzzzzzz upload next video.

[@SamirKhan-ee6bd]

Sir Please Make video for CSIR-NET series. It will rise your subscriber count

[@Ulululu670]

Where's the next part of this Video

[@ashishtanwar8088]

Good day,
sir you didn't mention about the property of eIF4B in this process.

[@hussainbiology]

. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

[@poojasonwane7919]

Sir can you please make a video on mitochondrial translation process

[@hussainbiology]

will consider Pooja

[@katecalimlim6655]

at 3:16 isnt it supposed to be elf5 not elf5b?

[@ammarahchaudhary2820]

How does cap independent translation occur plzzzzzz explain it also.

[@hussainbiology]

Will be explaining soon.
We had Internet Blockade from past week

[@bh5169]

amazing

[@ammaramehak9473]

sir you did not upload translation elongation and termination in eukaryote . plzzzzzzzzzzzzzzzzzzzzz explain post transcription modification along with group 1,2 ,3 4, .
i knew that i requested many videos and you fulfilled
thanx for uploading that realy it help me alot i have some problem in between that visero thatwhy i request you many time
sorry if you irritate with my repeatedly request .
your videos are ossum .

[@hussainbiology]

InshaAllah will be working on Post Translational Modifications from Next week.

[@hussainbiology]

There is no word of irritation but u need to understand as i am getting hundreds of requests to make videos on different niches , so i have to put forward everyone's request...Thanks and regards

[@hussainbiology]

Kindly Check the Animation of Translation where elongation and termination have been discussed in detail.

[@ammarahchaudhary2820]

Upload next video plzzz sir plzzzzzzz si

[@priyanshusharma2695]

Sir, when we get elongation and termination video of eukaryotes.

[@hussainbiology]

already available...Plz check the playlist

[@priyanshusharma2695]

Ok sir

[@priyanshusharma2695]

Sir can you please mention that links here of elongation and termination of eukaryotic translation...

[@ammarahchaudhary2820]

NIce

[@hussainbiology]

Thanks

[@anirudh_2OP]

Sir where is elongation and termination??? Plz rply

[@SathieskumarDuraisamy]

Subtitles should be given in english

[@hussainbiology]

okau

[@darshanadilhan5717]

Can u please add the vedios for translation elongation and termination

[@hussainbiology]

ua-cam.com/video/TkXEl9usLN4/v-deo.html

[@hussainbiology]

It is short video..but will work

[@darshanadilhan5717]

I am asking about Eukariyotic translation elongation not about prokaryotic translation elongation
Do they have the same mechanism?

[@hussainbiology]

@@darshanadilhan5717 ua-cam.com/video/i0PIsmxH07U/v-deo.html

[@hussainbiology]

This will help a bit till i make a complete detailed video

[@nehagohil4947]

👍

[@janvipatel1325]

Sir Aa eIF4A , eIF4E & eIF4G MATHI E, G BIND THAI TO 4A KAI

[@janvipatel1325]

Jay 6e

[@andreipotapenko8548]

dude, please work on your accent

[@hussainbiology]

okay sir

[@andreipotapenko8548]

@@hussainbiology thanks, brother. Once again, the content is great. But I can't understand anything.
Or, maybe, put some subtitles?

[@hussainbiology]

Yeah Surely.... I will work on accent as well as put subtitles

[@nadineboujday3023]

Could you please keep the English subtitles on? It helps me understand since I'm not a native English speaker, and I translate the words I don't understand.
Thank you

[@shortvideos1613]

Best molecular teacher ever 😍😍❤️
Thanku for sharing the video's,🙏🏻😍❤️

[@hussainbiology]

thanks for appreciation.. Glad it helps ✌️

[@misbashabir6514]

Thank you sooo much... But elongation and termination kte??

[@hussainbiology]

Playlist wuch... Tath menz sere video😊

[@hussainbiology]

email send krna apna ya kuch aur like insta or.. Jis pe mai woh aap ka doubt tha na.. i will make sure to send u those images regarding replication

[@hussainbiology]

ua-cam.com/video/i0PIsmxH07U/v-deo.html

[@misbashabir6514]

@@hussainbiology where I vll send yhii pe??

[@Jaybug20]

I’m studying 📑 for hussan

[@Jaybug20]

Okay I just wanted watch ❤

[@hussainbiology]

thanks brother...Keep sharing

[@rubiaghafoor3134]

This video helped me alot❤️

[@hussainbiology]

thanks for appreciation..Glad it helps

[@sajinasasi2603]

Thank u sir waitting next elongation and termination part

[@hussainbiology]

ua-cam.com/video/i0PIsmxH07U/v-deo.html

[@hussainbiology]

This animation will lead to Elongation and Termination...