Cell Division Time lapse
Вставка
- Опубліковано 12 бер 2017
- Time_lapse of cell division from second cleavage. The animal pole is clearly visible in the upper half of the image.
A brief note about the image capture techniques:
Hi I can say that it was done with a custom designed microscope based on the "infinity optical design" It is not available by any manufacturer. I built it. I used LEDs and relevant optics to light the egg. They too were custom designed by me. The whole microscope sits on anti-vibration table. I have to say that it doesn't matter too much what microscope people use to perform this. There are countless other variables involved in performing this tricky shot, such as for example: the ambient temperature during shooting; the time at which the eggs were collected; the handling skills of the operator; the type of water used; lenses; quality of camera etc etc. Hope this helps.
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At first I thought this was an animation. It's the first time I see this in such detail. Fascinating how the divisions move in waves through the egg.
Hi thanks. Yes it certainly is fascinating and of course not possible to visualize these "waves" with the naked eye or without a time lapse technique.
Needs a time lapse counter to see how much time it actually takes.
Maybe next time! Thanks for watching!
33 h ?
Yes it was a long time due to the cold temperature
Incredible footage!
Thanks!
Thanks for saying that and thanks for watching!
Great job on getting this footage and sharing it with everyone @francischeefilms! I really appreciate the effort and I'm happy I get to witness this amazing process
Hi thanks for saying so. I hope all can enjoy seeing this amazing process!
I love how each cell manages to divide at nearly the same time as the others
Awesome !!!! One of the best youtube video for me ! Thank's you so much !
glad u liked it! Thanks!
Thank's to YOU for the work ! (and the great idea!)
This is real?! Absolutely incredible!! Can we get some insight into the process behind this? what is the time frame involved in the sequence? Is it real time or over several minutes/days?
This is one of the most awesome and underrated video I've ever seen. I've sent it to every friend of mine but few people is able to apreciate it's beauty.
Hi, thanks so much for saying this. I shot another sequence last year similar to this but never posted it maybe I will now!
This is soooo Cool!✨😲
Such a great footage! Cant stop watching it.
Hi thanks!
Wow i thought this was animated at first!!! Amazing work dude.
amazing
can you show the phase where it implodes in on itself like a black hole when the division reaches a critical mass? I truly believe there is a connection between collapsing stars and black holes and the event which creates a life
awesome video, I can't wait to see more like this.
Thanks so much for saying this!
That's pretty amazing. I'll be showing this to my little science buddy geek, aka my son later. :)
Fantastic, would LOVE to know more technical details! Well done
Hi thanks
Fascinating. Hats-off to you for your custom setup build, looks like a 3D render... amazing!
Hi thanks. LOL, I wish I was this skilled with CGI, I'd probably be doing a different daytime job LOL! and more importantly wouldn't need to spends loads on microscope lenses!
I'll add this to my next filmmaking roundup on my site. Look forward to seeing more!
Hi thanks!
Great channel. Subscribed. Hope you get the recognition you deserve for this.
Hi thanks for subbing and thanks so much for saying this too. I hope you like what you see!
Could you patent this technique or share it with the scientific community perhaps? Documentary film-makers would love this sort of stuff
This technique is really something special. I can see the scientific and documentary communities going ape shit for this.
It's not patentable unfortunately. It is simply a microscope laid over, ie constructed in a horizontal format instead of a regular upright or inverted scopes. All the rest is entirely up to the skills of those hands setting it up. A bit like owing a RED and not being able to squeeze everything out of it to the advantage of the DOP etc.
Hey, I just want you to know that this video is computer animated the OP is lying about creating a special microscope. Imaging of this process at this scale would look nothing like this, and OP's description of his microscope is not consistent with actual optical principles.
Can you make a video from one cell to a living animal? I saw a video of a salamander growing like this video until it hatched. Unfortunately I cannot find that video again, but it was so amazing I could never forget it.
Wow. Thank you for this! It is mind blowing, that I live in a time, where I can just sit at home and see THIS! A question: Do you know what triggers the division? There is clearly an impulse and a chain reaction. Is there a single point of origin for this impulse? And is it know, what causes it? Thank you again, you are awesome!
Wow. it is very interesting.
It would be great to see such a video shot about Tardigrad, and then in an Internet a lot of graphics. But I did not see a real video about them
Mesmerizing to watch. Beautiful
Hi thanks!
Wow! Amazing!
Thanks
This is unbelievably satisfying. That little jiggle.
Why are there always some people who dislike something as astonishing as this? Humanity doesn´t need this kind of negativity ;)
Great video!
Thanks!
Because it is CG and the OP is lying about it being actual images.
You guys, believe it or not I even appreciate the trolls here like you, so thanks for watching!
Oh BTW, you only give me credit for CGI I'm not able to do LOL! But as I said thanks for watching!
For those who still doubt this is real.
Here's an old video with less high ress quality but showing the same thing:
ua-cam.com/video/dXpAbezdOho/v-deo.html
Thanks!
When compared to that video this one looks even more animated.
That sort of happens when you go hi-res. Compare old TV shows with Blu-ray movies and tell me actors don't look like artificial dolls with facial features painted on nowadays.
Yes you are totally correct. HD produces quality which is leaps and bounds greater than the old standard def TV and or video footage. Soon FCF will be shooting in 4K resolution, so this will be "leaps and bounds" above what you see now here. Old SD footage, you might "get away" with stuff being slightly out of focus, garbage being on your subjects etc etc but of course HD will reveal all. Your analogy to actors is so correct. Thanks for watching!
Another thing that makes things like this look animated? Timelapse, it's perfectly reasonable to expect this to be real.
Wow ! Amazing
Thanks!
Fascinating!
Thankyou!
This made my day
Thankyou.
Amazing contribution to science.
Many thanks.it was filmed with a microscope I designed and built.
kinda satisfied to watch. make mooooore
I'll do my best! Thanks for watching!
That is pretty cool.
Thanks
This is absolutely one of the most fascinating things I've ever seen.
Hi thanks!
the most amazing thing I've seen on yt
Thanks! and thanks for watching!
It's animated. Not real images, despite OP's claims.
素晴らしい！
Incredible video! And yet, as of this post, there were three people who hated it enough to give it a "thumbs down." I just don't understand people.
Hi thanks! Yes, I suppose there will always be people like that. I find there are many who like to "knock" my work, in regards to either SFX, sound, editing or whatever. I guess all I can say to them is I wish I also had that extra time to spend writing this stuff, I'd put it to better use LOL!
What imaging modality (light microscopy, macro lens, etc?) are you using to film this?
lies
@Kinjiro89 Ignorant.
Kinjiro89 it's real that's a frogs egg
they are using one of these www.edmundoptics.com/f/mitutoyo-infinity-corrected-long-working-distance-objectives/12298/
Looks like a tadpole egg to me. Pretty kewl to watch.
It is indeed!
Fantastic footage!
What's the measurement of the egg captured in this timelapse?
It would be fantastic to see more micro timelapse photography of these process that we rarely get a peek into... do you have any more future videos in the works?
Hi thanks for your comments. Yes I will publish the approx size, just need to calibrate the scope, sorry for this omission!
Good CG =)))
I'm afraid it' the real thing Nikita, not CG
Cool!
Thanks! I can do better, so stay tuned.
Is there a possible way to have a time lapse from the single egg cell to a tadpole that's ready to hatch?
Yes but the real trick is if you want to not have any cuts, then the embryo must be anchored. Stay tuned!
He's right, you need to anchor it in order to do it in one cut but it will be difficult due to movement. I have nearly perfected so stick around and I'll see what I can do.
francischeefilms please continue this work
Do you have behind the scenes photo/video or a photo of your microscope setup?
unfortunately no. Thanks for watching.
because it doesnt exist
+francischeefilms Could you take a photo of it not set up? Or any part of what you used?
Yeah we need more info to take this as reality
facebook.com/WildlifeAndScienceFilms/photos/a.421244081287411.94680.413320402079779/956243704454110/?type=3&theater
Фантастика!
love this! I want more!! I work in Medical animation. This is really lovely :)
Hi thanks! Stay tuned!
I subbed, you lot are very talented!
I look at this video and actually can't believe it's not CGI. :-) Especially the noise patterns that form during the division along the edges... This looks like some random, parametric mesh topology with displacement mapping.
Thanks!
It is CGI. The OP is certainly bullshitting. You cannot capture this type of activity with simple optical imaging. You need staining.
AMAZING! Please can you tell me what equipment you are using here? I have a microscope I can attach my camera to, but not to this degree :-o so cool, well done! :-)
looks interesting... It's dividing alright but why isn't growing? what cell is this?
A zygote. It doesn't grow because during this process, called cleavage, the mitotic divisions are so fast that the cells don't have time to achieve the growing phase in mitosis.
yes indeed.
It's also surrounded by a membrane that doesn't break for some time, holding the cells together while they divide.
I saw this video in the news few days ago and i thought that you might want to know! I'm from Poland
So cool
Thanks!
Iv seen your video on a couple online news outlets, though im sure youve seen them too, that might be partly why alot people seem to have seen this video in partictular.
Yes, I can't help that many of these places have simply taken the video without asking but yes it has really spread around now. Some unfortunately have put some text which is not quite right from a biological standpoint, oh well. That's the social media.
Can we see a photo or better yet a tour of your experimental setup?
I will indeed endeavour to do exactly that! So stay tuned!
Ok here is an old photo from taken from the setup used to shoot the second sequence I just uploaded
facebook.com/WildlifeAndScienceFilms/photos/a.421244081287411.94680.413320402079779/956243704454110/?type=3&theater
Search the FB page and you will find similar.
Maybe this will convince the doubters LOL!
Alright. You've convinced me. Great work. And great job you've made for yourself.
Could you make a video showing the setup you used to do this ?
Ah well I was waiting for someone to ask this. You know what i think I'll make a behind the scenes video so all can see. How dos that sound?
Very cool !
francischeefilms That sounds great. I hope to be convinced it's genuine and eat my hat. I'm half way to hat eating.
Hi thanks. LOL! It's like all the other clips here, all 100% the real McCoy, no CGI. LOL if only everyone knew what I know about CGI would have to be examined using a microscope LOL!
wow, that looks weirdly amazing... :D
I've found answers to the "how long was this" question, but still would like to know about the size of this thing, or rather, how much does the size change during the 33 hours?
in the video it of course looks like it stays the same (and the individual cells get smaller), which I'm guessing isn't true...
I know it'll sound ... demanding? ungrateful? but some sort of size scale would be nice... O:)
but even like this... really great to see, thanks for this ;)
Hi thanks for your comments. Think of cutting up a sphere, no matter how many pieces you cut it up into, the overall size will remain the same. Of course with an embryo developing there will be a stage where cell growth occurs and stuff will get bigger. I generally don't put scales on the clips but will try to indicate the scale on the next clips. I need to calibrate the microscope as it is a new setup anyway. Your comment is just a reminder for me to get a move on LOL!
huh?!? ok, I have some reading up to do... (biology isn't really my thing, just curiosity)
I was under the impression that it would be growing from the get go... *blush*
thanks again ;)
This is amazing. Any doubters of the legitimacy should check out his Facebook page to see photos of the setup. Can you please say what species of frog it is?
Excuse me.
i wanna make that movie.
How many minutes did you shoot?
Hello, it goes from one big cell to a lot of small cells ! Is the firs one a huge cell and the final ones, normal size cells ? Or is it small cells that will grow as the baby feed ?
yes it just keeps dividing but at the same time growing larger and differentiating into whatever organs will form.
Why are the cells getting smaller with each divide? Should they not remain the same and the overall entity get bigger?
During the video they are undergoing cleavage and actually producing more and more cells. Eventually, recognizable structures (such as the neural crest)- that will become the "backbone" will begin to be obvious. Many people have asked about this so i will endeavour to post a time lapse of the following development.
Great...
Arigatogozaimashita!
So cool, how did you take it?
There is a brief description in the video description. Thanks for watching!
Is the scale constant along this video? Or is it zooming out as the cells divide and then grow?
Constant scale.
Who on earth could dislike this video?
I don't know personally but there are quite a few "thumbs down". Maybe my jealous rivals LOL!
Thats beautiful, and better of all is this istn cgi, you can do more films like this, is very interesting and amazing
Thanks and I'll try and bring you guys and gals a lot more interesting imaging of biological behaviour etc! Thanks for watching.
"Not CGI"
can we see the morula at the end or it almost became?
not quite.
Am I the only one who’s trypophobia went CRAZY
Certainly not
npc
Where can I find out more about how this was made?
LOL by asking me of course!
Time lapse period 33 hour approx. at 15-17C filming started at the second cleavage. Dedicated custom designed microscope (sorry u can't buy one off the shelf) Optical system infinity optics but with modifications for a greater DOF.
I'll add more developmental series here soon, so stay tuned.
Can you show us the setup you used to make this?
Hi I can say that it was done with a custom designed microscope based on the "infinity optical design" It is not available by any manufacturer. I built it. I used LEDs and relevant optics to light the egg. They too were custom designed by me. The whole microscope sits on anti-vibration table. I have to say that it doesn't matter too much what microscope people use to perform this. There are countless other variables involved in performing this tricky shot, such as for example: the ambient temperature during shooting; the time at which the eggs were collected; the handling skills of the operator; the type of water used; lenses; quality of camera etc etc. Hope this helps. PS I have now placed this info in the description for all to see.
Thanks for the details!
No problems!
Wow!
Thanks!
Can you share the period of time this took real time?
about 33 hours at 15-17C approximately.
Temperature is important for the time it takes?
Is your video?
+Frederic Purenne At cell level it is
Yes it is.
Screw the haters man! This is absolutely amazeballs!!
Is it possible you could make another video further along the process? Perhaps at a larger, more associative size??
Cool vid never the less! 👍🏽
Hi see the next video I uploaded, it takes the viewer to the embryonic tadpole. Thanks for watching!
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licensing@storyful.com
They will be pleased to help you.
You may notice that each division round propagates like a wave. Is there some explanation why this happens like that?
Asking the real questions!
I'm sure this is known and it is of course a good observation of yours. Search Actomyosin driven contractile waves.
Aleksandr Zenin because it's fake
@lawrence Ignorant.
are the pulses generated from a single spot and then replicated to the rest of the cell?
o.o an alpha soul and millions of copies o.o
Hi to be honest I don't know yet. I need to do more reading and speak with the relevant people.
wow
Is this morula
The morula stage is present
Thanks for the nightmares. :(
Why isnt the embryo transparent?
There is a lot of yolk material in the lower half and the pigmentation of the cells present themselves as opaque. It's not like those human or chicken embryo images we are used to with back lighting. No amount of back lighting will penetrate this object. Although there are several other microscopy techniques such as confocal and two photon etc which can enable sub imaging to a much better resolution.
I would love to see the rest
ua-cam.com/video/fZQLhDHSa6Y/v-deo.html
How did you end up mounting the egg?
It was supported by others around it.
Why did they stop??
They didn't but the whole zygote went out of frame after approx 33 hours. So I did actually reposition the sample and kept recording however, the imaging was not very good, so it's simply not here. If it was to continue you would of course see the development of an embryonic tadpole, forming gills, u can see those gills on some previous footage of mine, here on this channel. Maybe i'll upload some of those stages as well. This Channel only shows a mere fraction of my work, just some stuff for everyone to see.
if it is cell division, why aren't the cells separated ?
Why would you think they should be separated? For example, we are undergoing cell division constantly (under control - we hope if we are healthy individuals), if every time a cell would divide and go off to do it's own thing then we would be a mess. What is happening here is known as cleavage and we start with one large cell, it cleaves, and cleaves and continues until it is impossible to distinguish individual cells. These cells go onto (under strict control) to start forming set structures at certain time periods, depending upon the temperature and other environmental influences. Eventually we see something that looks like a recognisable organism forming. ie neural crest gills, tail etc. Do not confuse this imaging with that of separating chromosomes.
the image is getting more and more pixels
In the clear part, where there is the incidence of light, it seems that the division is more intense than in the dark part of the egg. :)
The anterior pole or animal pole (at the top) is dividing more rapidly than the vegetal pole at the base.
or it's just a 3d render?
I wish I was that good, or even knew how to do that stuff LOL! Mind you I tried my hand at AE in an attempt to make realistic gunfire, LOL, what a joke it was!
Back Story? Who did the research?
I do all my own research. I do this kind of stuff for a living LOL!
francischeefilms you mean animating? Because this isn't actually real. You don't just custom build the most sophisticated, highest visual quality microscope and filming setup for a UA-cam video. If getting this level of quality at such a small scale was possible like this, then we would've seen it already from a reputable scientific organization, not some random person's UA-cam channel.....
francischeefilms you mean animating? Because this isn't actually real. You don't just custom build the most sophisticated, highest visual quality microscope and filming setup for a UA-cam video. If getting this level of quality at such a small scale was possible like this, then we would've seen it already from a reputable scientific organization, not some random person's UA-cam channel.....
Hey, this guy is full of it. This video is not of real cells. It is clearly animated. His microscope description shows that he doesn't understand the imaging process necessary to see dynamic activity on this scale.
So where is the growing part? All I see are divisions..
(I'm not trying to be rude, I just don't know..?)
Dupy, Dziary, Muscle Cary. it's because at this stage of development, the cells are dividing faster than they can grow. They eventually will though!
Why did you cut the recording?
it moved out of frame into a position that was not a good angle.
i think it's because he was too lazy to make more frames of the animation
Thanks for being a Troll, it keeps up my views! You see everyone gets a chance to comment, how's that for fair play eh?
Which species???
Rana temporaria
This is a rendered animation. Not real like the description suggests.
It is a very cool animation though.
Its not actually. I dont know why you would assume it is, considering its the egg of a frog, which starts off as a reasonably large single cell to begin with anyways.
is this x. laevis?
No it's R.temporaria
thanks! beautiful video.
poligonautas
so, that's how it happens
Yep! Indeed. Thanks for watching!
9gag brought me here. Amazing work .
so where's the frog?
In the garden of course LOL! Seriously the remaining development is taking place as I type this no more than 10m from my computer.
cool
Thanks for watching!
is this real? looks alien
Hi u bet!
Mitose or meiose
Hi please see this explanation, hope this helps
www.onlinebiologynotes.com/developmental-biology-of-frog-embryonic-development/
I don't know why, but this is disturbing me at a visceral level...
Ok - for all those doubters here. I have known Francis for 25 years, I have seen his set-up and discussed his microscope, lighting and lensing design over years. He was a scientist and professional microscopist in his past career, and he has been working on this for years.This is 100% real. Congratulations.I can also confirm that CGI at this Level is way beyond him!! Hahaha
LOL! There you go everyone!
It’s not hard to find Francis’s publications in a Google Scholar search. But the thought of Francis doing this with cgi if hilarious. Given the options of coding the light field required to get the defocus effect, or just using a microscope to film it in real life, I know which option he’d take.
Why is this so uncomfortable lmaoo
Really amazing! However the article I read on Science Alert (from facebook) mentioned it divided from 4 cells into several million within the 20 seconds. I counted the number of ripples and it seems to come to 11, that would make for 8,192 cells... not millions.. did I miss something?
Hi I'm not sure what developmental phase they were talking about from that FB reference but what we see here is cleavage at this early stage and the processes you see are very much temperature dependent. if that article is about this actual footage then they have made an error in their description.