Royal society of chemistry is my favorite channel. your explanation is excellent. i started youtube channel by inspiration of your channel. bless me i did video on column chromatography
If the fractions are all the same color, how do you differentiate them from one another? Even after using TLC beforehand to see how many fractions there are, you cant tell when one stops and another starts.
yes you can. You do a tlc of the different fractions side by side on one sheet with the raw substance as reference on every sheet. Than you see which fraction is pure and which are mixed fractions. Otherwise you couldn't seperate colorless substances.
@@Chem-iu5jx Another option; if you know what you're working with, could be smell. When producing anethole, it's very easy to recognize where the product is by smell.
@@Chem-iu5jx How do you know when to change fractions? :) it's chaos really, you need to be lucky, or you can take 1000 different fractions with like 2 drops in each :D
@@AndyU96 Noooo depending on your column size you adjust the volume of the fractions you collect. For example for 8g of compound I use a column with a diameter of roughly 3cm. The collected fractions are 20ml. YOu have to choose a solvent system were you get a R_f of under 0.5 otherwise won't work well. And I collect between 20-100 fractions :D really depends.
thank you, is there standard to use silica gel in column, I mean if my sample a little bit like (0.01 gram), how much of silica gel use here? in contrast, if I have a lot of samples such as (1 gram or more), here, how much of silica gel have to use? I mean is there ratio from silica gel to sample to use in column, please tell me
The higher the packing of your column with silica, the better is your separation of side products. So you chose the amount of silica based on the complexity of the necessary separation. You should perform TLC prior to column chromatography to check how much the spots of product and side products are apart. The more crude product you have the wider your column should be. If you only have 100 mg, you can easily use a column as shown in the video. With 1 g of crude product, I would personally use a wider column.
Eluent will vary depending on your compound you'll have to TLC your compound prior to your column to find out which "eluent" or solvent system will give you good separation try 50:50 ethyl acetate petroleum ether aim for an Rf around .2 if 50:50 doesn't work either make it more polar (increase ethyl acetate) or less polar (add more petroleum).
In the column chromatography, If stationary phase is silica gel, the mobile phase is the mixture (ratio of petroleum ether: ethyl acetate is 9:1 ). The sample include compound A and compound B. IF the polarity of A is great than the polarity of A. Which compound will be eluted first. Please explain the reason.
If our stationary phase is polar we use a non polar or less polar solvent so if a component A in the sample is polar than B so A will be obtained later and the latter will be obtained first since our solvent is non polar so B flows faster than A which is more polar..... I hope it was helpful❤️
Can any person who is knowledgeable in this field because I ain’t no chemist unfortunately and a chemist help would be appreciated. Is what this guy is doing is he setting it up to extract materials of any kind or is he extracting something specific... because I want to extract cbd/ from thc ... no I’m not a drug king pin I discard the thc in a safe place ;;) wink lol I am a medical card holder ...
Got a satisfactory video to clear my theory doubts. Great work🙂🙂
Awesome and very informative thank you! I’m doing a column chromatography experiment separating the compounds In red wine today in lab 😁
Oh nice, I hear there’s a bit of GHB present in that (red wine). That’s gammahydoxybutyric acid. That’s one of my favorite drugs.
Royal society of chemistry is my favorite channel. your explanation is excellent. i started youtube channel by inspiration of your channel. bless me i did video on column chromatography
Good video, I showed this to my girlfriend to explain what I do all day haha
bdbdbd are u a pharmacist ??
Maria Iqbal medicinal chemist
I am showing it all my 'non-chemistry' friends 😁
you are so humorous ^^
@@snehajavenkatesh8412 good........
Good one...
My man showed me this video and I can understand what he's doing all day...
It requires lot of hard work 💯
Hats off to him ❤
Why do you mix all of them if then you want to separate them?
😂😂😂
😂 actually tho
This is a procedure to demonstrate how to separate compounds in a mixed solution,hence why he mix all of them
Good teaching resource
Isn’t the Eluent what comes out of the column?
you forgot to mention putting air down the top to force the eluent down faster
If the fractions are all the same color, how do you differentiate them from one another? Even after using TLC beforehand to see how many fractions there are, you cant tell when one stops and another starts.
yes you can. You do a tlc of the different fractions side by side on one sheet with the raw substance as reference on every sheet. Than you see which fraction is pure and which are mixed fractions. Otherwise you couldn't seperate colorless substances.
@@Chem-iu5jx Another option; if you know what you're working with, could be smell. When producing anethole, it's very easy to recognize where the product is by smell.
@@Chem-iu5jx How do you know when to change fractions? :) it's chaos really, you need to be lucky, or you can take 1000 different fractions with like 2 drops in each :D
@@AndyU96 Noooo depending on your column size you adjust the volume of the fractions you collect. For example for 8g of compound I use a column with a diameter of roughly 3cm. The collected fractions are 20ml. YOu have to choose a solvent system were you get a R_f of under 0.5 otherwise won't work well. And I collect between 20-100 fractions :D really depends.
@@Chem-iu5jx I need to be your friend
I use glass wool..
Long live this clarification
What did he use the sand for?
what are the compounds seperated in experiment?
Can you please tell regarding ideal size of silica?
Usually 50-70 times as your product weight.
Measure for preparation 😮
Thanks because of your nice information, would you please, say, why we should add sand?
To protect silica uniformity from impact of pouring eluent
thank you, is there standard to use silica gel in column, I mean if my sample a little bit like (0.01 gram), how much of silica gel use here?
in contrast, if I have a lot of samples such as (1 gram or more), here, how much of silica gel have to use?
I mean is there ratio from silica gel to sample to use in column, please tell me
The higher the packing of your column with silica, the better is your separation of side products. So you chose the amount of silica based on the complexity of the necessary separation. You should perform TLC prior to column chromatography to check how much the spots of product and side products are apart. The more crude product you have the wider your column should be. If you only have 100 mg, you can easily use a column as shown in the video. With 1 g of crude product, I would personally use a wider column.
480p is the best we could do in 2016?
O my God I can't get Australian accent 🤣🤣🤣🤘🤘
can you show me what is the eluent thanks
Hi Duong Huy, the eluent is the solvent used to run the column
Eluent will vary depending on your compound you'll have to TLC your compound prior to your column to find out which "eluent" or solvent system will give you good separation try 50:50 ethyl acetate petroleum ether aim for an Rf around .2 if 50:50 doesn't work either make it more polar (increase ethyl acetate) or less polar (add more petroleum).
@@jimmymc4752 After watching the video, I understood that you didn't mean "tender loving care" when you wrote TLC.
In the column chromatography, If stationary phase is silica gel, the mobile phase is the mixture (ratio of petroleum ether: ethyl acetate is 9:1 ). The sample include compound A and compound B. IF the polarity of A is great than the polarity of A.
Which compound will be eluted first. Please explain the reason.
If our stationary phase is polar we use a non polar or less polar solvent so if a component A in the sample is polar than B so A will be obtained later and the latter will be obtained first since our solvent is non polar so B flows faster than A which is more polar.....
I hope it was helpful❤️
According to the rule like dissolves like non polar component will be more soluble in the non polar solvent..
What tipe of eluent dou you use?
It‘s better to use a face mask when you handle silica gel.
nobody, and i repeat literally nobody, in any research laboratory around the world does this.
EDIT: That's what the fumehood is for.
very clear thanks
why added sand? wont it block the sample ? and did you mix silica with distilled water?
Hexanes
@@BearYourCross ur mum
@@habibitalbiii I'm offended
@@BearYourCross same
Which kind of sand i can use
Isn't that Dr. David Reed narrating?
could barely see the fractions and the colors. the quality was bad
Thanks sir
Is there a protocol for the polarity of eluent? Can Rf from tlc help in knowing the polarity of eluent?
Can you do this to extract cbd/ from thc
I don't know much about chemistry but I'm pretty sure you can just extract both individually
Yes you can buy you have to figure out what mobile phase and stationary phase you want to use first.
good
Just tryna figure out how to make ricin smh
It's very hard to actually see what's happen for some reason. I believe the camera quality wasn't up to par
Can any person who is knowledgeable in this field because I ain’t no chemist unfortunately and a chemist help would be appreciated. Is what this guy is doing is he setting it up to extract materials of any kind or is he extracting something specific... because I want to extract cbd/ from thc ... no I’m not a drug king pin I discard the thc in a safe place ;;) wink lol I am a medical card holder ...
Online class chemists whew
We are not satisfied with your experiment lamo🤣🤣🤣
Ppl
P
boring
You clicked on this, dumbass
@@juliof.5080 Yes, he did so to find out that's boring.