How to Prepare Stained Temporary Mount of Human Cheek Cells | Human Chick Cell (ENGLISH)

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The tissue that lines the inside of the mouth is known as the basal mucosa and is composed of squamous epithelial cells. These structures, commonly thought of as cheek cells, divide approximately every 24 hours and are constantly shed from the body. Cheek cells secrete a continuous supply of mucin, the principal element of mucous. In combination with the salivary glands, the mucin maintains a moist environment in the oral cavity. A sufficient moisture content must be continually sustained in order to aid enzymes in softening food, facilitate swallowing, and initiate the process of digestion. Cheek cells are eukaryotic cells (cells that contain a nucleus and other organelles within enclosed in a membrane) that are easily shed from the mouth lining. It is therefore easy to obtain them for observation. Some of the main parts of a cell include;
1. The cell membrane (outer boundary of the cell)
2. Cytoplasm (the fluid within the cell)
3. A nucleus (at the center of the cell and controls cell functions)
4. Organelles (e.g. mitochondria-Organelles are cell structures with specific functions)
Using biological stains such as methylene blue, it is possible to clearly observe and differentiate the different parts of a cell. This is because the stain will color some parts of the cell and not others, allowing them to be clearly observed.
Methods-
(1) Take a clean cotton swab and gently scrape the inside of your mouth.
(2) Smear the cotton swab on the centre of the microscope slide for 2 to 3 seconds.
(3) Add a drop of methylene blue solution and place a coverslip on top.
Concentrated methylene blue is toxic if ingested. Wear gloves and do NOT allow children to handle methylene blue solution or have access to the bottle of solution.
(5) Remove any excess solution by allowing a paper towel to touch one side of the coverslip.
(6) Place the slide on the microscope, with 4 x or 10 x objective in position and find a cell. Then view at higher magnification.
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two Siddha and Ayurvedic preparation with their indication

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