HPLC TIPS FOR ROUTINE PROBLEMS | VOICE OF KAYANI
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- Опубліковано 22 сер 2024
- HPLC TIPS FOR ROUTINE PROBLEMS | VOICE OF KAYANI
This video based on HPLC tips for routine problems.
in this video explained 10 important point and tips for HPLC problem and solution.
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Hplc vials ko wash karne ka best tarkika
Keep dipping in solvent for washing your company using ..which is validated cleaning procedure...and use methanol also for washing and dry in over.....
Will make a video again on it
Sir standard or test solution kaise calculate kare is pr video banaye
Ok done
Sir hplc ki 2nd means organic solvent washing ke case me solvent kis base par decide hota hai ? .....kya sabhi cases me methanol use karte ? Hai ?
Agar mobile phase Mai methanol use huwa hai to organic phase methanol ka hoga....and agar acetonitrile use huwa hai to organic phase actronitrile use hoga...
Normally depends k Kiya solvent use huwa hai HPLC analysis Mai
@@voiceofkayani5419 but sir is it true that acetone or acetonitrile is not good for hplc ...it may degrade column ?
Hume hplc column Kab aur kaise discard karna chahye ...
Ok the basis of theoretical plats
@@voiceofkayani5419 no. Of injection ? Based ?
Sir ...kya uv calibration ki frequency kya hai ? Kya ye fix hoti hai or company wise hoti hai
Fix as per best practices....
6 months calibration frequency
@@voiceofkayani5419 any guideline or practice based ?
@vasudevsharma-ey6ni based on best practices of multinational pharmaceutical industry and you can check WHO , ICH GUIDELINES and also ISO 17025
Sir GC mai sample inject karne ke lie moisture ki kya limit hai?
I mean kitna percent moisture ho to sample inject kar sakte hai?
It should be under 1‰
It should be under 1 %
@@voiceofkayani5419 sir agar moisture jyada ho to kya koi impact hota hai
@vasudevsharma-ey6ni affects the analysis
Agar internal diameter increase Kar de to RT par kya Asar hoga
RT pe asar nai parega. But peak broad ho jaegi
@@voiceofkayani5419 aur particle size increase kare to kya farak pdega