Hi! Great video. When you are analyzing a set of images and want to compare spot counts, this value below the filter tab, which is the bottom left diagram's threshold - would you keep it as generated automatically or adjust and use the same manual value for all the images? Hope it made sense)) Thanks!!
Decon is fine as long as it follows the rules. If not you'll end up with artifacts and you might end up chasing ghosts. You need to have a well matched refractive index, you'll need a relatively thin sample (not something I'd advise for tissue if you want to be strictly quantitative), nice specific labeling, and good signal to noise.
Hi! Great video. When you are analyzing a set of images and want to compare spot counts, this value below the filter tab, which is the bottom left diagram's threshold - would you keep it as generated automatically or adjust and use the same manual value for all the images? Hope it made sense)) Thanks!!
thanks! was this image deconvolved before doing the isospot analysis or is that not recommended?
Decon is fine as long as it follows the rules. If not you'll end up with artifacts and you might end up chasing ghosts.
You need to have a well matched refractive index, you'll need a relatively thin sample (not something I'd advise for tissue if you want to be strictly quantitative), nice specific labeling, and good signal to noise.