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Our invert zoo (Invertebrate Zoology) professor mandated the tardigrade searching - she made us look for them as part of the course work. Being my major advisor on top of that, I ended up doing a literature review for her for my (Marine Biology) capstone about...essentially the likelihood of finding novel species in the rocky intertidal of the state we're in. Which she later did. I have, of course, shared some of your tardigrade episodes with her.
James is so clever! I wish that his next book could be a series of protocols for us other amateur microscopists to replicate some of his amazing observations!
It really is a simple as: 1) put water in a container, 2) expose water to radiation between infrared and UV, 3) ensure some degree of fresh air to water interface, 4) algae appears out of nowhere! Obviously, there are some unspoken additional rules like, your water can’t be in solid form, your air shouldn’t contain a high percentage of methane, and so on. But really, don’t overthink it!
Bucket of water windowsill some fertiliser a peice of already that you want to proliferate. I assume. Not a microbiologist just a housewife but if I wanted some already that's what I'd do.
@@ChloroplastspectrumI'd assume putting a bit of the already species you want to grow would be helpful. Like we have some red lager here which would grow just as well as green sheet algea whatever that's called ...the little slimey seaweed stuff... I'd probably put a bit of the green algea in the glass so that it would grow that and some fertiliser for it to consume like a bit of leaf would do ...I've got many buckets in my garden and they all grow different algeas ...the red one I think is an algea but might be a mold bit it grows under water so I'm not sure... but it's always interesting to see what grows in my buckets ...the cleanest buckets grow the red stuff the ones with some leaves in the bottom usually grow the green fast seaweed looking stuff and some grow a brown algea which just clings to the sides in sort of hair like filaments looks the most gross of all.
I’ve been trying to find tardigrades for more than a year and now it’s summer so all moss has dried 😭 will have to try in winter unless I manage to find some moss earlier, thank you so much!!
the tardigrades are probably still there local mosses to areas that dry out will wake up when given rain water, the tardigrades will wake up with the moss i expect i have never looked for tardigrades because i do not have a microscope but i do collect mosses for my tiny paludariums and i wouodn tbe surprised to find some tardigrades if i do ever check under a microscope (even if i do live in the driest part of europe)
I just adore the way they look, so many microscopic creatures are either weird orbs or long worm shapes, but tardigrades *look* like a standard animal, legs, feet, a head, body sections etc. they look like a little mammal-caterpillar creature! They’re just such cool little guys
As soon as it realised it's predicament the beast let out a mighty roar and threw it's bodyweight against the bars making the wranglers step back and hold their cattle prods a bit higher.
Super to see yet another use for the common windowscreen! I do so enjoy seeing average, everyday items being used in cooler, better ways. 😊👍🏼 And super to see Hank back up to full-steam ahead, as well. Warms the cockles of my heart - and any number of deities know that it's hard to keep one's cockles warm during an 🥶 icy cold polar vortex. 🥶 I mean, come on, -1°F -18.3°C should not be allowed to be THE HIGH in the middle of MO, USA! Still, Hank can definitely warm these cockles. Such an excellent voice for this work, and anyone can tell he loves what he does. That makes viewers like me love what he does, too. 😊
tardigrades are such cool creatures! by far one of my favourite microscopic organisms, especially seeing as most other organisms the same size are parasites or other dangerous stuff XD
Used a much, much, much larger Baermann funnel to harvest nematode larvae from our cultures for research. Never thought about using one for samples from nature.
I just ordered some cheese mesh. Ill be giving this a go. Love looking at little critters with my son under the microscope. Quick question: I assume tap water will be bad? better to get bottled water?
Tap water has chlorine in it for the express purpose of killing micro organisms. It's part of what keeps our water safe! Regular bottled water might still be chlorinated, make sure to get "distilled" water. I would recommend getting distilled water, or getting fish "water conditioner" to add to a bucket of tap water.
@vahannema Yes that would also be fine as long as the groundwater has not been treated by the public water services. (Again, when they treat water they add chlorine which is totally fine for humans but bad for microbes) Rainwater would definitely be fine
You can also dechlorinate tap water by leaving it in the sun or under a UV light for a day or two. But this can be risky as there is no way to tell how much chlorine is left in the water at any given point. As well as a mosquito risk. (I know all this because of a fishkeeping hobby)
What if we trap them, sort them according to size and breed the largest for 100 generations? Grizzly water bears? Moss boars instead of moss piglets? Where would it end?!
so sad catching this channel on the way out. the interest and passion for the subject make these videos so pleasant to watch as someone with no prior privy to such subjects. your quality is astounding, it is disheartening to learn you are departing from us, i hope your future endeavors bring you more fulfillment and happiness
Amazing footage! What equipment are you using here? I've never seen videos so sharp before, it's as if I am seeing into the microscope through my screen!
The final picture, around the 8 minute mark - the white parts - by any chance are those suspected microplastics? Are those issues with samples James collects?
I've cultivated a small but healthy community of tardigrades. I check on them daily and try to add fresh lichen or moss regularly, as well as distilled water. One problem I am having though is a biofilm layer keeps settling on the surface and I'm afraid it will choke out the oxygen from the water. Any ideas on how to deal with the biofilm?
I have to admit I'm struggling to understand just how James could collect the sample from the funnel? Maybe I'm simply missing a fundamental step in here but it seems like what we've got is a funnel open at the top, stoppered at the bottom, correct? And the organisms are settling to the narrow end after passing through the mesh. But to sample that, you have to open the bottom, don't you? So - how do you ensure that not ALL the water comes at once? It seems like it'd make it very hard to get a sample from the actual bottom if you just let all the water into a vessel? The way you described it, it just seems like James was able to sample directly from the funnel's narrow part and that's where I get confused. I'd be thrilled with a "tutorial" video of sorts showing us some more of the care-and-feeding, and sampling, that James does. I recall seeing a couple of other videos where we saw how he keeps his samples and creatures alive and that was VERY cool. Learning the basics would make me feel a lot more confident in trying this myself. I've been frankly doubtful that I could find anything alive at all, simply because the creatures would expire before I fumbled my way to a usable slide!
It looks like they didn’t use much water, enough that it washes the microbes out, but not so much that the moss is soaking in the water you want to look at. The mesh and the moss can be lifted up out of the funnel, or the container underneath can be pulled away, making it easy to pipette it onto slides. Hope this helps! Good luck with your observations
I use your microscope and when I found my first tardigrade it was either dead or in cryptobiosis. But at the moment I was sad and excited at the same time. Then I took a great video of a tardigrade I named Sally. I used one of the rheinberg filters and it made her look bright blue. I know you will be ending the channel at the end of the year. But please keep all the videos up so we can still watch and share them. Thanks,.
Oh crap. I collected some moss-water about 6 weeks ago and stored it in a sealed tube but have been busy and haven't got around to looking at a sample under the microscope. I fear I have caused a Tardigrade massacre 😢 I thought they could survive outerspace. Shizzle.
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Can I use a T shirt to replace the filter? I tried a tea bag but it broke into pieces when I tried to cut it to flatten it out.
So cute, the tardigrade’s feet over the glass slide. 😁
Our invert zoo (Invertebrate Zoology) professor mandated the tardigrade searching - she made us look for them as part of the course work. Being my major advisor on top of that, I ended up doing a literature review for her for my (Marine Biology) capstone about...essentially the likelihood of finding novel species in the rocky intertidal of the state we're in. Which she later did. I have, of course, shared some of your tardigrade episodes with her.
I don't know why I'm up at three in the morning watching this, but it seems really cool! Thanks for sharing it with us.
Same! lol
This channel is peak sleepy time vibes tbh
It's because of your overwhelming quest for knowledge!!💖
All trapping must be done in accordance with state laws, regulations and licensing requirements.
i think the master of tardigradeis in europe
Microscopic laws written in microscopic documents
The fun part is applying the tardigrade tags to your catch.
@@pauljs75Dyetag. Seen people tag gmo crops.
What's the quota this year? 😁
This type of practical information is immeasurably helpful and interesting as well. Thanks for letting us look over your shoulder.
So amazing getting to see all the variety in a single slide 🤯😍❣️
Pls create the first microscopic zoo to visit in the entire world!
The chill music in the background elevates this video to a new level, watching that footage was awesome!
James is so clever! I wish that his next book could be a series of protocols for us other amateur microscopists to replicate some of his amazing observations!
I would love to see more on how James is able to raise the algae for food.
It really is a simple as: 1) put water in a container, 2) expose water to radiation between infrared and UV, 3) ensure some degree of fresh air to water interface, 4) algae appears out of nowhere! Obviously, there are some unspoken additional rules like, your water can’t be in solid form, your air shouldn’t contain a high percentage of methane, and so on. But really, don’t overthink it!
Bucket of water windowsill some fertiliser a peice of already that you want to proliferate. I assume. Not a microbiologist just a housewife but if I wanted some already that's what I'd do.
@@ChloroplastspectrumI'd assume putting a bit of the already species you want to grow would be helpful. Like we have some red lager here which would grow just as well as green sheet algea whatever that's called ...the little slimey seaweed stuff... I'd probably put a bit of the green algea in the glass so that it would grow that and some fertiliser for it to consume like a bit of leaf would do ...I've got many buckets in my garden and they all grow different algeas ...the red one I think is an algea but might be a mold bit it grows under water so I'm not sure... but it's always interesting to see what grows in my buckets ...the cleanest buckets grow the red stuff the ones with some leaves in the bottom usually grow the green fast seaweed looking stuff and some grow a brown algea which just clings to the sides in sort of hair like filaments looks the most gross of all.
Nicely done!
Thank you for talking about the funnel. I found a truly amazing, prehistoric looking Tardigrade last summer. I’m going to try this when it warms up.
Those little bears are so cute! 🥰
“Microbiologists hate him! Catch tardigrades easily with this one simple trick!”
microcosmos after a joint is just nuts
I love the detail so we can try this at home.
I’ve been trying to find tardigrades for more than a year and now it’s summer so all moss has dried 😭 will have to try in winter unless I manage to find some moss earlier, thank you so much!!
the tardigrades are probably still there
local mosses to areas that dry out will wake up when given rain water, the tardigrades will wake up with the moss i expect
i have never looked for tardigrades because i do not have a microscope but i do collect mosses for my tiny paludariums and i wouodn tbe surprised to find some tardigrades if i do ever check under a microscope (even if i do live in the driest part of europe)
Mr. Green, thank you for this great channel. A great escape from OUR world.
I just adore the way they look, so many microscopic creatures are either weird orbs or long worm shapes, but tardigrades *look* like a standard animal, legs, feet, a head, body sections etc. they look like a little mammal-caterpillar creature! They’re just such cool little guys
really enjoyed this, a tecnique i used at uni, then promptly forgot about in my lab job.
This is really great! Thanks
I'm 100% going to try this! 😍👏🏾
These videos are so relaxing
Thanks! Im gonna go try this now!
So this is a Water Baermann funnel, since it captures water bears.
Tartigrades so cool!
Morning coffee and microbes. Sounds like the day is off to a great start 😁
As soon as it realised it's predicament the beast let out a mighty roar and threw it's bodyweight against the bars making the wranglers step back and hold their cattle prods a bit higher.
I use Kimwipes as my mesh which keeps the excess soil out while it filters.
Ooo, I've gotta try this ASAP
Really appreciate your videos !!
We need a tardigrade emoji!
gotta try this once it thaws up, thanks!
You guys are great.
I stayed up for this Hank your welcome
My calendar arrived! Oh, happy day!
Using the Baermann funnel to trap some water bears is really funny to me
Super to see yet another use for the common windowscreen! I do so enjoy seeing average, everyday items being used in cooler, better ways. 😊👍🏼
And super to see Hank back up to full-steam ahead, as well. Warms the cockles of my heart - and any number of deities know that it's hard to keep one's cockles warm during an 🥶 icy cold polar vortex. 🥶 I mean, come on, -1°F -18.3°C should not be allowed to be THE HIGH in the middle of MO, USA!
Still, Hank can definitely warm these cockles. Such an excellent voice for this work, and anyone can tell he loves what he does. That makes viewers like me love what he does, too. 😊
Way to go James!
Omg they're adorable ❤
this is some CRISP footage
Thanks! I have been searching for water bears for years. Now I will try 😊 😂
Useful stuff thankyou...I wonder if tardigrades from different locations react to each other differently to ones from the same place?
Once the debris was cleared, the screen looked like it was full of squirming pickles.
tardigrades are such cool creatures! by far one of my favourite microscopic organisms, especially seeing as most other organisms the same size are parasites or other dangerous stuff XD
Appreciate this channel soo much. Awesome content. Been subbed for a while and can see the improvement in this channel.
Used a much, much, much larger Baermann funnel to harvest nematode larvae from our cultures for research. Never thought about using one for samples from nature.
Their flaps and folds are so cute
I wonder how many I've eaten by accident lol
Cool video! I'll try trapping some and filming them in my lab! Tardigrades are still missing from my video collection right now...
Thank goodness for James
Amazing. A whole world going on beyond our sight. Imagine what lives inside our own bodies, thinking we are their planet. 😁
Amazing … 👍👍👍❤️❤️❤️⭐️⭐️⭐️
I just ordered some cheese mesh. Ill be giving this a go. Love looking at little critters with my son under the microscope.
Quick question: I assume tap water will be bad? better to get bottled water?
Tap water has chlorine in it for the express purpose of killing micro organisms. It's part of what keeps our water safe!
Regular bottled water might still be chlorinated, make sure to get "distilled" water.
I would recommend getting distilled water, or getting fish "water conditioner" to add to a bucket of tap water.
what about rain- or groundwater?
@vahannema Yes that would also be fine as long as the groundwater has not been treated by the public water services. (Again, when they treat water they add chlorine which is totally fine for humans but bad for microbes)
Rainwater would definitely be fine
You can also dechlorinate tap water by leaving it in the sun or under a UV light for a day or two. But this can be risky as there is no way to tell how much chlorine is left in the water at any given point. As well as a mosquito risk.
(I know all this because of a fishkeeping hobby)
Can we all just agree that uplifting tardigrades would be awesome in every way?
The feet are soooo cute!! 🥰
Been screaming at the void about political issues for an hour, so this is exactly what I needed
Tardigrades are immense.
Tardigrades run !
Yes, have used funnel. Some samples released a lot of debris.
my 2k monitor just shines when watching this channel in 4k resolution.
MAGNIFICENT!
Baermann was born in Breslau, former Prussia, now Poland. He worked for the former Dutch East Indies administration as a foreign scientist.
I just had cookies with strawberry milk
nice 👍
That’s dope
awesome
Strawberry cookies as well?
Your mother
So cool
What if we trap them, sort them according to size and breed the largest for 100 generations? Grizzly water bears? Moss boars instead of moss piglets? Where would it end?!
so sad catching this channel on the way out. the interest and passion for the subject make these videos so pleasant to watch as someone with no prior privy to such subjects. your quality is astounding, it is disheartening to learn you are departing from us, i hope your future endeavors bring you more fulfillment and happiness
Amazing footage! What equipment are you using here? I've never seen videos so sharp before, it's as if I am seeing into the microscope through my screen!
Tardigrades are unironically cute
I love tardigrades
Cosmo Sheldrake, the Tardigrade Song
Wooow, which microscope and camera ?
wow!
what sort of microscopy do you use in this video?
also welcome back hank!
They are too cute, please treat them accordingly!
Tardigrades can survive the vacuum of space and make a good replacement for goldfish.
🤩😍🤩 gorgeous beasties!!! If you add water, use rainwater or reservoir water. Tapwater these days can kill...a lot of us. 🙏
The final picture, around the 8 minute mark - the white parts - by any chance are those suspected microplastics? Are those issues with samples James collects?
I've cultivated a small but healthy community of tardigrades. I check on them daily and try to add fresh lichen or moss regularly, as well as distilled water.
One problem I am having though is a biofilm layer keeps settling on the surface and I'm afraid it will choke out the oxygen from the water. Any ideas on how to deal with the biofilm?
What are the tiny ones zipping around in the background?
What is the “inch worm” microbe at 6:20?
A rotifer. They move about that way before opening up their cilia and doing their thing.
I'm planning to resume my tardigrade hunt after it thaws outside, which will be in a few days! Do you recommend any microscopes for amateurs?
Foldscopes are perfect for amateur microscopists!
tardigrade pet tardigrade pet tardigrade pet tardigrade pet
This whole time I didn’t know it was Hank Green narrating! and now i’m just like “duh!” of course that’s why the voice sounds so familiar you dunce!
Entonces así es como el canal más serio de Biología conoció al tardiluche
Oh whoops, I thought this was a mix-tape 😂😂😂
Which camera and microscope do you use to obtain these images? Could you please share them? Thank you
Water Bear Trap 🪤 (imagine that emoji is a bear trap... y'know, with a spring-loaded jaw and sharp teeth and a chain)
I have to admit I'm struggling to understand just how James could collect the sample from the funnel? Maybe I'm simply missing a fundamental step in here but it seems like what we've got is a funnel open at the top, stoppered at the bottom, correct? And the organisms are settling to the narrow end after passing through the mesh. But to sample that, you have to open the bottom, don't you? So - how do you ensure that not ALL the water comes at once? It seems like it'd make it very hard to get a sample from the actual bottom if you just let all the water into a vessel? The way you described it, it just seems like James was able to sample directly from the funnel's narrow part and that's where I get confused.
I'd be thrilled with a "tutorial" video of sorts showing us some more of the care-and-feeding, and sampling, that James does. I recall seeing a couple of other videos where we saw how he keeps his samples and creatures alive and that was VERY cool. Learning the basics would make me feel a lot more confident in trying this myself. I've been frankly doubtful that I could find anything alive at all, simply because the creatures would expire before I fumbled my way to a usable slide!
ohh I just suck the bottom of the funnel with another pipette!
It looks like they didn’t use much water, enough that it washes the microbes out, but not so much that the moss is soaking in the water you want to look at. The mesh and the moss can be lifted up out of the funnel, or the container underneath can be pulled away, making it easy to pipette it onto slides. Hope this helps! Good luck with your observations
Are tardigrades actually transparent or do they seem this way because there is a bright light behind them?
going to use Cheese cloth, plastic funnel and moldable rubber erasers for the bott
om
I wonder how many tardigrades we consume in processed foods
I get the joke, a "tun" of tardigrades. Good one...
I use your microscope and when I found my first tardigrade it was either dead or in cryptobiosis. But at the moment I was sad and excited at the same time. Then I took a great video of a tardigrade I named Sally. I used one of the rheinberg filters and it made her look bright blue. I know you will be ending the channel at the end of the year. But please keep all the videos up so we can still watch and share them. Thanks,.
I NEED A TARDIGRADE TRAP SO I CAN HAVE ALL THE PET TARDIGRADES I COULD EVER WANT!!!
Oh crap.
I collected some moss-water about 6 weeks ago and stored it in a sealed tube but have been busy and haven't got around to looking at a sample under the microscope.
I fear I have caused a Tardigrade massacre 😢
I thought they could survive outerspace.
Shizzle.
Hey when are you guys going to get more of those microscopes in stock I check every so often to see if they're in stock but they never are.
Aw the little one on the glass died :(
What is a good but reasonable camera to use? I have a bresser 5mp camera, but the quality is well bow what i can see in the view finders.
Cosmo Sheldrake brought me here.
Let’s go