@@MedicinalChemistryCenter thanks. I tried this but the cells were floating. I need to differentiate them into M1 and M2 macrophages. Can you help me with tips please?
@@ridwanibrahim202 Each task has been implemented in our laboratory. We used this protocol www.sigmaaldrich.com/technical-documents/protocols/biology/cell-culture/pbmc-macrophage-differentiation.html?gclid=CjwKCAjwkN6EBhBNEiwADVfyaw29lC0dREN9cfLNBONh7C5DC5yZgFoftColdwQNY6nmty5OwuYhlBoCEpIQAvD_BwE Or www.sigmaaldrich.com/technical-documents/protocols/biology/cell-culture/pbmc-macrophage-differentiation.html?gclid=CjwKCAjwkN6EBhBNEiwADVfyaw29lC0dREN9cfLNBONh7C5DC5yZgFoftColdwQNY6nmty5OwuYhlBoCEpIQAvD_BwE
I have a question after pouring the blood in an angle on above the ficol reagent or histopaque, when I make it straight I feel that some blood gets draw or mix with the ficol reagent or histopaque like very minute. Will that be a problem??
good video, where are the other parts. My question is after isolating the PBMCs, do you plate then on coated plates or non plated plates are fine?
Thanks for your question! After isolation, cells are best cultured on uncoated plates (untreated)
@@MedicinalChemistryCenter thanks. I tried this but the cells were floating. I need to differentiate them into M1 and M2 macrophages. Can you help me with tips please?
@@ridwanibrahim202 Each task has been implemented in our laboratory. We used this protocol www.sigmaaldrich.com/technical-documents/protocols/biology/cell-culture/pbmc-macrophage-differentiation.html?gclid=CjwKCAjwkN6EBhBNEiwADVfyaw29lC0dREN9cfLNBONh7C5DC5yZgFoftColdwQNY6nmty5OwuYhlBoCEpIQAvD_BwE Or www.sigmaaldrich.com/technical-documents/protocols/biology/cell-culture/pbmc-macrophage-differentiation.html?gclid=CjwKCAjwkN6EBhBNEiwADVfyaw29lC0dREN9cfLNBONh7C5DC5yZgFoftColdwQNY6nmty5OwuYhlBoCEpIQAvD_BwE
@@MedicinalChemistryCenter thank you very much. I would check it out tomorrow
The bottle on the righ which marked by EDTA 0.5 mM for what, what thats meant??
Hey. This is a solution of EDTA in phosphate buffer, concentration 0.5 mmol / l
@@MedicinalChemistryCenterOur laboratory mix blood with NS,so what difference are they ?
I have a question after pouring the blood in an angle on above the ficol reagent or histopaque, when I make it straight I feel that some blood gets draw or mix with the ficol reagent or histopaque like very minute. Will that be a problem??
Dear Colleague,
You must be careful and slow when layering blood on the ficole. Slight sagging of the ficol layer is not critical.
Good luck to you.