Calculating oligo water volume

When you obtain oligos from IDT, typically they will be lyophilized (dried out). So, you must resuspend the oligos in ddH20. First you need to calculate how much water to add. The math comes out as multiplying the "nm" number on the tube by 10 to get the number of microliters of water, and the result is a 100uM stock.

[@ArnavPadhi]

do the primers always come in nm moles when they are in lyophilised form??

[@lilpiggie2167]

I do not know if all oligo suppliers express the amount of material in the tube as nanomoles (nm). IDT does. If you were to synthesize oligos yourself, you would not have this number. Instead, you would measure the UV absorbance of a solution of the oligo, and calculate the molarity of the solution from this measurement.

[@ArnavPadhi]

@@lilpiggie2167 okay

[@jonnywalker2015]

How did you get quick multiplication by 10 again?

[@marcopel83]

Hey?! Aren't you wearing your lab coat? :)