Mouse Genotyping

( www.abnova.com ) - Genotyping is the process of determining the genes (genotype) of an individual by examining its DNA with the use of biological assays (ex: PCR, DNA sequencing). Three steps to type the mouse genotype : (i) isolate the genomic DNA from mouse tails; (ii) PCR to amplify with specific primers; (iii) electrophoresis to determine the genotype. More videos at Abnova ( www.abnova.com ).

[@syedjamal3262]

Great video but please have the PCR results explained.

[@1986mayday]

How do you choose the primers for PCR? I know that they must target the sense and antisense strands of your gene of interest, and that the melting temperatures are nearly the same, but how do you find primers that meet these specifications?

[@vinnied7]

Your thumbs are contaminating the sample.