Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
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Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Thank you for being so clear in your explanation, super helpful video. I have a question; how can we use the CreloxP system to restrict a particular gene knockout to a certain stage of development (i.e. adolescence)?
You need to use the cre ER system . Tamoxifen inducible Cre-estrogen receptor (ER) fusion protein. For this, Cre is fused with the ligand-binding domain of a mutated ligand-binding domain of ER, which does not bind to estrogen but binds to tamoxifen with high affinity. Cre-ER proteins are sequestered in the cytoplasm via association with the HSP90 chaperone. Upon addition of tamoxifen, tamoxifen-bound Cre-ER dissociates from HSP90, translocates into the nucleus, and carries out site-specific recombination between flanking
Amazing video!! I have a question, so in the LoxP mouse, all the tissues will have the gene you want to delete flanked by LoxP sites. And only in tissues where Cre recombinase is active will you have the gene deletion occurring. Do the LoxP sites around the gene interfere with the correct transcription/translation of the gene in tissues where Cre recombinase is not expressed? I am wondering whether the LoxP sites could interfere with the translation of the gene.
Please make it a detailed version of this video! You have a great concept on these topic but when you are teaching you are jumping into related topics very often thats makes understanding quite difficult! Please make a detailed video! I request you! Thank you sir!
Hi, can the cre recombinase travel to other tissues (even though it has a tissue-specific promoter) and knock out the GOI e.g. the cre is brain-specific but can it travel to the liver and knockout loxp flanked gene of interest? And is the loxp flanking also tissue-specific?
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Please support our channel by using super thanks. Your 2$ donation would give us motivation to do more. Super thanks option is present below any of my videos, a heart sign with $ in it.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
hola, podrías ayudarme, como resolverias este problema: “Usted generó un ratón KO para el gen XYZ. Luego de la obtención de las quimeras usted obtuvo ratones XYZ+/-, los ratones XYZ+/- son viables. Al criarlos se obtuvieron crias con la siguiente frecuencia: 50% XYZ+/+, 25% XYZ+/- y 25% XYZ-/-, pero los ratones KO mueren a los pocos días de nacer. Usted decidió hacer un knock in para el gen XYZ, con el fin de verificar los tejidos en los cuales se expresa, encontrando que se expresa en el hígado, páncreas y riñón. ¿Cuáles son los pasos necesarios para obtener un ratón donde el gen XYZ solo se elimine del páncreas? Esquematizando los vectores que se utilizarán, indicando todos los elementos importantes para la transgenesis.”
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@@ManuelGomezLopez-wf5is Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Thank you for making this video! It is by far the best one I have found on the topic of what Cre Lox system is and how it works.
please share my channel link with your friends
I was very confused about using TAM for activating cre.
You made it very clear.
Thanks a lot.
youtube.com/@letscrackbioexams94?si=ZWdPXv2NSpZeDHZu please subscribe to my second channel 🙏
Thanks!
Recived with thanks. Please suggest topics on which you want to see videos
Your visual and simple explanations are so amazing! Thank you for your channel.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Why is this technique not given a Nobel yet?
No idea
nobel prize is no longer for true scientist, it is for corporate scientists that's why
The best explained of the system I have heard, thank you
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Super well-explained video! I hope you are my professor teaching me!
Please support me by subscribing my second channel youtube.com/@letscrackbioexams94?si=l2Di6Uc4hCYYa3ve
The explanation is quite clear
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Wow , one of the best video on cre lox p . Thank you so much ... I understood 😊😊 #from UoH
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Thanks for this video. Best explaination I have found.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Thanks for explaining the application. 🙏🙏
Please share my channel link with your friends and help me to reach big audiance.
Thank you for making this very simple! Very helpful!
please share my channel link with your friends and help me to reach big audiance
Thank you for being so clear in your explanation, super helpful video. I have a question; how can we use the CreloxP system to restrict a particular gene knockout to a certain stage of development (i.e. adolescence)?
You need to use the cre ER system . Tamoxifen inducible Cre-estrogen receptor (ER) fusion protein. For this, Cre is fused with the ligand-binding domain of a mutated ligand-binding domain of ER, which does not bind to estrogen but binds to tamoxifen with high affinity. Cre-ER proteins are sequestered in the cytoplasm via association with the HSP90 chaperone. Upon addition of tamoxifen, tamoxifen-bound Cre-ER dissociates from HSP90, translocates into the nucleus, and carries out site-specific recombination between flanking
@@animatedbiologywitharpan Thank you for your help!
Please share my channel link with your friends and help me to reach big audiance
Thank you for the nice video. Could you please explain once how the LoxP mouse would be generated?
yes i would make a video on that
Amazing video!! I have a question, so in the LoxP mouse, all the tissues will have the gene you want to delete flanked by LoxP sites.
And only in tissues where Cre recombinase is active will you have the gene deletion occurring.
Do the LoxP sites around the gene interfere with the correct transcription/translation of the gene in tissues where Cre recombinase is not expressed? I am wondering whether the LoxP sites could interfere with the translation of the gene.
Good question, in most of the cases it does not interfere. The loxP sites are small.generally they flank exon and not in orf.
Can you also discuss how does Off-target recombination can occur with Cre? I think its also called Cre leakiness... I'm still confused
OK I will make a video on that
Please make it a detailed version of this video! You have a great concept on these topic but when you are teaching you are jumping into related topics very often thats makes understanding quite difficult! Please make a detailed video! I request you! Thank you sir!
Thanks for the detailed feedback
Thank you my friend, great explanation!!
Hi, can the cre recombinase travel to other tissues (even though it has a tissue-specific promoter) and knock out the GOI e.g. the cre is brain-specific but can it travel to the liver and knockout loxp flanked gene of interest? And is the loxp flanking also tissue-specific?
No it can't
Why the phosphodiester bond on the 2' carbon
In KPPC models what does it mean when they say there's Kras recombination or P53 recombination?
I am not sure about this
clear explanation thank you
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
thank you! better than me prof definitely lol
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Please share my channel link with your friends and help me to reach big audiance
so good thank you
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Thank you man!
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very nice explanation
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Can you explain how AAV might be used with CRE-loxP sites
I will try to make a video on that
hola, podrías ayudarme, como resolverias este problema: “Usted generó un ratón KO para el gen XYZ. Luego de la obtención de las quimeras usted obtuvo ratones XYZ+/-, los ratones XYZ+/- son viables. Al criarlos se obtuvieron crias con la siguiente frecuencia: 50% XYZ+/+, 25% XYZ+/- y 25% XYZ-/-, pero los ratones KO mueren a los pocos días de nacer. Usted decidió hacer un knock in para el gen XYZ, con el fin de verificar los tejidos en los cuales se expresa, encontrando que se expresa en el hígado, páncreas y riñón. ¿Cuáles son los pasos necesarios para obtener un ratón donde el gen XYZ solo se elimine del páncreas? Esquematizando los vectores que se utilizarán, indicando todos los elementos importantes para la transgenesis.”
Incredible video !!! Thank u very much for this clear explanation !! ❤
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nice video
@@ManuelGomezLopez-wf5is Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
E knockout rat killer cake 3, 4 packs thinty chachipotara emaina effect untada leda chepandi
I have no clue
break it down for me.. so cre is what, a virus? and when does one use cre-lox or creER-lox? thanks
Cre is an enzyme ( it's a recombinase enzyme). Cre loxp system can be used to knockout genes.
If one person can eat this knockout rat killer cake 3, 4 , packs what will happens
I haveno clue