2 Protein Analysis using Tandem Mass Spectrometry

Mass Spectrometry has transformed the analysis of proteins in the past 3 decades. In the second of thirteen introductory seminars, Rod Chalk head of mass spectrometry at Oxford's Centre for Medicines Discovery discusses the challenges involved in fragmentation analysis of proteins and how they have been overcome. Watch time 47 minutes.

[@TanTan-ch3vq]

Best explanation for protein analysis by MS, thank you so much!

[@rodchalk9945]

Glad it was helpful!

[@TanTan-ch3vq]

Thank you, Doc! Recently, I tried a LC-MS analysis on my protein sample and found that the machine detected a lower value than the expected mass (22KD), about 2.5 dalton lower than theoretical value. Is this normal for ion trap MS? thanks

[@iot3136]

Thank you Dr. Chalk. You are a great educator. Learned a lot. If you could please do an another video on comparison of DDA and DIA/SWATH. Would be really greatfull.

[@isabelacopetti404]

This was very helpful! Thank you very much

[@rodchalk9945]

You're very welcome!

[@nastaranhdz1529]

Thank you so much, I learned a lot!

[@rodchalk9945]

You're very welcome!

[@user-sx9tk8lq5z]

What type of software do you use for protein analysis