6:04 If my calculations are correct, if you have a viable cell count of 200,00 cells/mL in a total volume of 20 mL, then that should be 4,000,000 cells. If you then want to to seed 10,000 cells into the new flask (0.0025 or 0.25% of the total cells), you should transfer 0.05 mL (50 mcL) of the original sample into the new flask.
6.07/6:05: Formula= Desired number of cells to seed/Total number of cells=Volume to transfer/Volume of the cell suspension 10000/4000000=volume to transfer/20mL 10000/4000000*20=0.05mL which is equal to 50 microliter Further Total cells=4000000 and total volume is 20000ul so 1ul contains= 4000000/20000=200 which multiply by 50= 50*200=10000 cells
6:04 If my calculations are correct, if you have a viable cell count of 200,00 cells/mL in a total volume of 20 mL, then that should be 4,000,000 cells. If you then want to to seed 10,000 cells into the new flask (0.0025 or 0.25% of the total cells), you should transfer 0.05 mL (50 mcL) of the original sample into the new flask.
totally agree
Plz tell me
Total procedure of platelets counting
Uhhhhhhhhh Abcam made a mistake
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thanks...
6.07/6:05: Formula= Desired number of cells to seed/Total number of cells=Volume to transfer/Volume of the cell suspension
10000/4000000=volume to transfer/20mL
10000/4000000*20=0.05mL which is equal to 50 microliter
Further
Total cells=4000000 and total volume is 20000ul so 1ul contains= 4000000/20000=200 which multiply by 50= 50*200=10000 cells
ua-cam.com/video/GCohewzhD3k/v-deo.html, For the 2nd fomular, are you sure you want to use count1 to divide by 4, not count5 divided by 4?
I think is count5 divided by 4
For anybody stressing over the calculations at the end, these are wrong.