And what about analyses where the IC-values are classified as "ambiguous"? I just analyzed some data with an R^2 of 0.91 (not excellent, but not horribly bad!) where the results shown are labelled as "ambiguous" and the numbers given in the ECF field are just impossible (>100x the highest concentration used). What is the problem?
Hello and thanks for your question. This web page explains what "Ambiguous" in Graphpad prism curve fit mean: www.graphpad.com/guides/prism/latest/curve-fitting/reg_analysischeck_nonlin_ambiguous.htm
Your videos are very helpful. Could you also upload a video to determine Km of a substrate when are i substrate titration and absorbance as my reading?
Hello and thanks for your question. This web page explains what "unstable" in Graphpad prism curve fits mean: www.graphpad.com/guides/prism/latest/curve-fitting/reg_unstable.htm
Thank you Sir. This video saved me lots of extra calculations.
I am glad you liked it
And what about analyses where the IC-values are classified as "ambiguous"? I just analyzed some data with an R^2 of 0.91 (not excellent, but not horribly bad!) where the results shown are labelled as "ambiguous" and the numbers given in the ECF field are just impossible (>100x the highest concentration used). What is the problem?
Hello and thanks for your question. This web page explains what "Ambiguous" in Graphpad prism curve fit mean: www.graphpad.com/guides/prism/latest/curve-fitting/reg_analysischeck_nonlin_ambiguous.htm
Thank you, nice tutorail.
Glad it was helpful!
Your videos are very helpful. Could you also upload a video to determine Km of a substrate when are i substrate titration and absorbance as my reading?
Good idea. I will upload a video soon. Thanks
Thanks, this helped me a lot :)
I am glad you liked it
What if the answer comes in as unstable?
Hello and thanks for your question. This web page explains what "unstable" in Graphpad prism curve fits mean: www.graphpad.com/guides/prism/latest/curve-fitting/reg_unstable.htm
THANKS!!!
You're welcome!