🎯 Key Takeaways for quick navigation: 00:00 🧬 In vitro digestion studies simulate physiological digestion conditions, including enzymes, pH, digestion time, and salt concentration. 00:45 💊 Researchers adapt in vitro digestion protocol to develop evidence-based method for adjusting pancreatic enzyme therapy in cystic fibrosis. 02:08 🍽️ In vitro digestion process involves oral, gastric, and intestinal stages, mimicking mastication, stomach acid, and bile salt effects. 03:37 🧪 pH adjustments during digestion stages critical for accurate enzyme activity and lipolysis measurement. 06:21 ⚙️ Centrifugation and lyophilization separate undigested solid phase from digested micellar phase for further analysis. 07:59 🧪 Lyophilization process removes water from digested samples, enabling quantification of free fatty acids and lipolysis. Made with HARPA AI
Saludos desde Quito Ecuador. Tenemos un proyecto con un componente de simular digestión in vitro de un alimento para niños. Queremos aplicar el protocolo de INFOGEST. A qué correo puede contactar ?
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So helpful, very clear description of the process. Thank you
🎯 Key Takeaways for quick navigation:
00:00 🧬 In vitro digestion studies simulate physiological digestion conditions, including enzymes, pH, digestion time, and salt concentration.
00:45 💊 Researchers adapt in vitro digestion protocol to develop evidence-based method for adjusting pancreatic enzyme therapy in cystic fibrosis.
02:08 🍽️ In vitro digestion process involves oral, gastric, and intestinal stages, mimicking mastication, stomach acid, and bile salt effects.
03:37 🧪 pH adjustments during digestion stages critical for accurate enzyme activity and lipolysis measurement.
06:21 ⚙️ Centrifugation and lyophilization separate undigested solid phase from digested micellar phase for further analysis.
07:59 🧪 Lyophilization process removes water from digested samples, enabling quantification of free fatty acids and lipolysis.
Made with HARPA AI
Truly incredible! Thanks for the video!!
Good morning,
I was wondering which kind of shaker incubator (brand, model) you were using in your experiment
very great and clear description of the method
What is the brand of the thermostat chamber used in this video? Thanks for sharing
Hi, where can i read the process for the preparation of the reagents? Thanks
Saludos desde Quito Ecuador. Tenemos un proyecto con un componente de simular digestión in vitro de un alimento para niños. Queremos aplicar el protocolo de INFOGEST. A qué correo puede contactar ?
May I ask what are the measurements for creating the Stimulated Gastric Fluid and Stimulated Intestinal Fluid?
The information is in the INFOGEST static in vitro simulation of gastrointestinal food digestion, 2019 by André Brodkorb
HAVE ANY SCIENTIFIC ARTICLE, I WOULD LIKE TO READ IT.
Hi! You can see the list of our scientific publications here www.mycyfapp.eu/index.php/en/dissemination-area/scientific-publications.
So helpful