I have a "chicken or egg" type question! where does the uniprot database come from? if it is the mass spectrometer that gives the sequence information, does the uniprot database derive from mass spec data? I also have another question; is uniprot different from a spectral library database for data independent acquisitions?
@@leoalmeida2583 Nesvizhskii, A. I. (2010). "A survey of computational methods and error rate estimation procedures for peptide and protein identification in shotgun proteomics." J Proteomics 73(11): 2092-2123.
Very nice explanation and good references!
I have a "chicken or egg" type question!
where does the uniprot database come from? if it is the mass spectrometer that gives the sequence information, does the uniprot database derive from mass spec data?
I also have another question; is uniprot different from a spectral library database for data independent acquisitions?
Great explanation. helps a lot
Which software you have used for matching the fragment ion of peptide?
They are mainly manually annotated. Otherwise, I have used Peaks Studio since v4.5 (~10+ years).
Can you please name that 2010 paper?
Can you be more specific?
@@MatthewPadula I wanted to read the paper at 13:20 but I could not find it on the internet.
@@leoalmeida2583 Nesvizhskii, A. I. (2010). "A survey of computational methods and error rate estimation procedures for peptide and protein identification in shotgun proteomics." J Proteomics 73(11): 2092-2123.
@@MatthewPadula thank you very much. I started my journey into proteomics and your channel has been very useful.
@@leoalmeida2583 Excellent. Don't be afraid to ask more questions. Either here or via email.