How to Culture Yeast? Part 1 - Harvesting and Rinsing Yeast

Hey everyone, this is our guide on how to culture yeast! In this video, we go over how to harvest and wash your yeast. We also talk about the importance of sterilisation and how to autoclave jars. Hope you found this video helpful and that you enjoy it! Part 2 will be released very soon, covering yeast starters and culturing your yeast for your brew day.
We hope to upload 1-2 videos weekly, so stay tuned and subscribe and like the video!
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Cheers!
Chapters:
0:00 Intro
0:36 Equipment
2:59 Harvesting Yeast
6:58 What to do after harvesting?
9:31 Rinsing Scenarios
10:58 Sterilising/Disinfecting Jars
12:52 DON'T BURN YOURSELF
13:08 Finished Sterilising Jars
13:42 First Yeast Rinsing
17:08 Second Yeast Rinsing
20:22 To be continued...

[@Jimsmith656]

I used to rinse but now I just over build a starter and save some for next time. Saves me rinsing . Awesome video though, honestly cant believe you guys dont have more subscribers. some of the better brewing content on youtube

[@flyingwombattv]

Cheers mate! Yeah we are slowly getting there, help us out and share our channel around with your brewing mates hahaha

[@vikramjitsingh4538]

This is the best video I have seen on Yeast rinsing..............its a bit of work but you can get many generations of reuse with a single pitch of yeast..............cheers

[@flyingwombattv]

Cheers dude glad you enjoyed it 👌🤘 yup way more bang for your buck

[@Ben54871]

Thanks for the awesome video and showing all the steps! Cheers!

[@flyingwombattv]

Glad you enjoyed it mate! Cheers

[@GabeVega-db4pv]

Municipal tap water contains chlorine and passes through legions of bacteria before it discharges the faucet - wouldn't recommend it, but if it's all you have, shake (aerate) and cap, then burp after 24 hours - majority of chlorine should be removed. While sterilizing the glassware, the cap is part of the containing vessel; recommend this gets cleaned as well.

[@flyingwombattv]

Yeah the cap was forgotten in the midst of filming hahaha it’s hard to maintain proper protocol whilst trying to talk to a camera

[@NoMusiciansInMusicAnymore]

I have been using pre boiled water, cooled in sealed mason jars to wash my yeast, does that cut it in your opinion? I haven't had any issues so far but I am still pretty new to this black magic. I usually do it straight from the packet in an overbuilt starter, does that make much difference? My logic here is I have to start the process a little earlier but i can take 1 pack, breed it up to say 4 packs, keep 2 in the fridge and use 2 for the brew, after that I can use the 2 in the fridge to make 4 and keep 2... am I just setting myself up though?
Thanks heaps for the video, would really appreciate a similar vid from packet to over built starter if you think it's not too samey?

[@flyingwombattv]

Yeah mate totally agree, can’t remember if I said it in part 1 or part 2. But I think the whole making a home “yeast bank” is an awesome idea. Make enough starter to keep as a “mother culture” that you can keep growing for future batches.
Honestly at the home brew scale it’s a bit of “if it works it works” hahaha, a proper brewery would opt for distilled water over boiled, but the general idea is to use as sterile water as possible. So honestly your probably fine

[@Ardyrezv]

Is there a home brew method to measure the number of vital cells after washing your second generation yeast?

[@flyingwombattv]

Only way that I can think of would be a cell count. You could probably get a pretty cheap second hand microscope online, but honestly for the homebrew level it’s probably overkill. I’d say make a new starter with your 2nd gen yeast and get it nice and healthy before pitching to your next batch

[@jaqhass]

Separation is easioer than washing. Did you try a centrifuge?

[@flyingwombattv]

Yeah we have a centrifuge, just different ways of doing things, most home brewers don’t have access to a centrifuge so we did this instead

[@jaqhass]

Okay. Fair enough. @@flyingwombattv

[@Mark-jq6en]

Please don't rinse your yeast, all you're doing is lowering osmotic pressure and contaminating your yeast.

[@flyingwombattv]

Cheers for the thoughts, yeah there’s always the chance of introducing contamination, which is why we say use distilled water to do this. And yes your are lowering osmotic pressure but unlike mammalian cells, yeast cells do have quite robust cell walls. In the scheme of things you arnt causing significant damage to your culture. I’ll take the benefits over the cons in this case