Can one simply take fresh seeds from an aquatic plant (Aponogeton) and germinate, grow them in a nutrient-rich tissue culture gel, even without all of the sterile lab equipment and procedures? I have an abundance of seeds which would be much easier to recruit than slicing and sterilizing bulb chunks to put into a sterilized MS medium.
@@xPorsum I have worked with Orchid seeds too. If you get them out of capsul you need to sterilise them and if in green capsule, you need to extract them without any contamination.
Generally pretty good, but I take issue with two things. At one point, the worker dips a piece of pH paper into the media, which is potentially a source of contamination. It probably won't make a difference, but the better way to do that is to dip a clean stirring rod into the media, and then touch the stirring rod to the pH paper. The more serious mistake was when the technician touched the 0.22 uM filter with his bare hand to insert it into the plastic filter cassette. Assume that your hands are full of bacteria. Since both sides of the filter are now assumed to be contaminated, bacteria will get into the solution after it passes through the filter. This should be done instead using a sterilized set of forceps, or better yet, buy pre-sterilized disposable filter cassettes to attach to syringes. Probably you'll get away without problems most of the time, but why risk it? The best single strategy in tissue culture is paranoia.
@@drpk6514 If you're doing filter sterilization, it's because the component (eg. acetosyringone) is heat labile, and has to be added after the media has been autoclaved and cooled. Hands in general are considered sources of contamination, so a good working rule is that your hands never touch the experiment. This is also a good safety practice. For the same reason, there is no justification for dipping pH paper directly into any solution. Probably the amount of chemical leached from the paper into the solution is not going to matter, but why add another unknown to the experiment when there is a cleaner way to do it?
@@brianfristensky521 There is absolutely no problem with using pH meter in the media. We are dealing with plants and they are not all that sensitive. The filter which was used for the filtering has 2 protective layers, the protective layers were placed on the hand to show the filter to the camera. Furthermore, these days no one uses these types of filters. They come in one time use ready to use one piece. BTW that "worker" has a PhD in plant biotechnology and this is his job.
What is that filter tool called?; the one used at the end of your video for cold filter sterilization it looks like a female nozzle tip for your syringe. I need to sterilize thidiazuron.
Yes they don't sell to individuals easily. I tried to buy something for myself but later they reviewed my purchase and cancelled it. However you can buy pretty much everything from eBay or smaller companies online. You could even buy prepared medias online easily.
@@drpk6514 thank you dr.karbaschi .i thought that you have some tools that translate them . now im writing and translate them .but its very hard for me.i think that i can do it on 20 hours. i am a retired teacher and i want I want to work on the propagation of 'salab'plant. see you
@@drpk6514 hi doctor Please help me if you have any information on the propagation of the 'salab' plant is named : Orchis caspia from orchis family or Orchidaceae
top notch educational content right here!
this video is actually really interesting
虽然我听不懂，但是我还是把它听完了，我从视频中能感受到您对工作的一种科学与严谨，谢谢您的分享。
Thanks for nice video! 🙂
Thank you very much from Yemen
concise but very useful information about plat tissue culture
Thank you.
the big question how many suckers can I get from a single plant?
Big help! Thank you!
please share other videos of this serie? Could you share the name of this video courses?
Can one simply take fresh seeds from an aquatic plant (Aponogeton) and germinate, grow them in a nutrient-rich tissue culture gel, even without all of the sterile lab equipment and procedures? I have an abundance of seeds which would be much easier to recruit than slicing and sterilizing bulb chunks to put into a sterilized MS medium.
If you do that then you will have a microbial bloom in the media and soon takes over your seeds/small plants.
@@drpk6514 but orchids are grown from seed in the same way so I'm sure it is possible. Maybe will have to tweak the gel mixture though
@@xPorsum I have worked with Orchid seeds too. If you get them out of capsul you need to sterilise them and if in green capsule, you need to extract them without any contamination.
thank you, so grateful
thank you ...
Thank You!
Generally pretty good, but I take issue with two things. At one point, the worker dips a piece of pH paper into the media, which is potentially a source of contamination. It probably won't make a difference, but the better way to do that is to dip a clean stirring rod into the media, and then touch the stirring rod to the pH paper.
The more serious mistake was when the technician touched the 0.22 uM filter with his bare hand to insert it into the plastic filter cassette. Assume that your hands are full of bacteria. Since both sides of the filter are now assumed to be contaminated, bacteria will get into the solution after it passes through the filter. This should be done instead using a sterilized set of forceps, or better yet, buy pre-sterilized disposable filter cassettes to attach to syringes.
Probably you'll get away without problems most of the time, but why risk it? The best single strategy in tissue culture is paranoia.
You know that the media will be autoclaved in the end right??
@@drpk6514 If you're doing filter sterilization, it's because the component (eg. acetosyringone) is heat labile, and has to be added after the media has been autoclaved and cooled. Hands in general are considered sources of contamination, so a good working rule is that your hands never touch the experiment. This is also a good safety practice.
For the same reason, there is no justification for dipping pH paper directly into any solution. Probably the amount of chemical leached from the paper into the solution is not going to matter, but why add another unknown to the experiment when there is a cleaner way to do it?
@@brianfristensky521 There is absolutely no problem with using pH meter in the media. We are dealing with plants and they are not all that sensitive.
The filter which was used for the filtering has 2 protective layers, the protective layers were placed on the hand to show the filter to the camera. Furthermore, these days no one uses these types of filters. They come in one time use ready to use one piece. BTW that "worker" has a PhD in plant biotechnology and this is his job.
What is that filter tool called?; the one used at the end of your video for cold filter sterilization it looks like a female nozzle tip for your syringe. I need to sterilize thidiazuron.
what kind of microscope which is behind the mam
I have a biological microscope, I imagine they use the same type of microscope in this video
BTW Sigma won't allow you to buy media / chemicals for home users. I tried
Yes they don't sell to individuals easily. I tried to buy something for myself but later they reviewed my purchase and cancelled it.
However you can buy pretty much everything from eBay or smaller companies online. You could even buy prepared medias online easily.
I ended up going to Phytotech labs instead. Pretty much have everything you'd need for plant tissue culture.
@@fortuneflux As I said you can use other online stores.
hi dr.karbaschi is it possible for me to see three basic tissue calture under persian noses?
Hi Esmail,
What exactly you mean?
@@drpk6514 im a kordish from iran and i dont know english well so i want you to send me persian translat of Basic plant tissue cultur 3 vidoes.
@@اسماعیلشریفی-م3ل Do you really expect me to translate 3 videos and send them to you because you dont know Engish? Get some books and read them.
@@drpk6514 thank you dr.karbaschi .i thought that you have some tools that translate them . now im writing and translate them .but its very hard for me.i think that i can do it on 20 hours.
i am a retired teacher and i want I want to work on the propagation of 'salab'plant.
see you
@@drpk6514 hi doctor Please help me if you have any information on the propagation of the 'salab' plant is named : Orchis caspia from orchis family or Orchidaceae
سلام وقت بخیر ببخشید چندتا سوال داشتم میشه راهنمایی کنید؟
Salam Befarmaid
ببخشید در مورد کشت بافت برای گیاه فیلدندرون چه لوازمی نیازه
واینکه مواد داخل ظرف کشت چه چیزایی هستن
شما کدوم شهر تشیف دارین؟
what pesticides and herbacides do you use? natural or chemically synthesized?
This is tissue culture so you don't need any pesticide/herbicide.
@@drpk6514 I think It Is posed a trick question. As the only pesticides involved are the ones produced by the plants themselves :)
8:55 you mentioned you use herbicides and such .?! Maybe that was what he was referring to.
The shocking lack of PPE