most of the dyes i bought from sigma aldrich or alfa aesar. Most of the video used: 9,10-diphenylanthracene 9.10-bis(phenylethynyl)anthracene rubrene rhodamine B tetraphenyl porphyrin and the special dye at 8:00 is a type of FRET based molecular beacon coded to the DNA of e.coli. you can buy the kit online but the compound itself is propriety so even I don't know what it actually is.
Is there such a thing as a single source of info, and encyclopedia of dyes, that shows all of their absorption and emission Spectra, compatibility ,chemical structure solubility etc... I would like to transfer animated gifs to t-shirts, the animation achieved by cycling long wave shortwave and RGB illumination.. 30 years ago I wanted to do animated flame effects on the hood of my car, but the one UV-c only activated red that I could find that was compatible with clear coat and had longevity was horrendously expensive.. If I can find a more imaginative artist to help me, perhaps a phosphorescent stegographic latent image you might see for a second before your eyes adjust 😜
Best intro video on Fluorescence, HANDS DOWN. This is how every professor should introduce this topic in chemistry courses. This was interesting, simple and concise. Great job Nurdrage!
What's the matter _you never had Europium Tetrakis Dibenzoylmethide Triethylammonium before?_ It's delicious. I drink it with my mountain dew so that when I use the bathroom at night I can see where I'm peeing.
i am so glad this channel is somehow still going. i used to watch so much of it as a kid and was looking for this video specifically because it always amazes me and is a great demonstration. thank you nurdrage for helping people see the cool side of chemistry and make them want to learn! i'm more into computer science now but chemistry will always be fascinating to me.
NurdRage, I've watched a lot of science videos on youtube, but yours are some of my personal favorites. You cover not only interesting topics but actually teach something in the process. Kudos, man.
Automotive Technicians also use fluorescent dyes to detect coolant leaks. A small amount of dye in a radiator and a UV light lets you see where coolant is coming from. It's a handy tool to have.
This is one of the coolest videos I've seen in a while. I really appreciate that you explained luminescence mechanism the proper way with energy diagrams without dumbifying it.
Too be honest i don't know. Most amateurs i find get their equipment from ebay. I get mine from professional distributors like VWR. I also get glassware from chemglass. But to be really honest chemistry as a hobby is NOT cheap. Glassware is expensive and breaks easily and often. Chemicals get used up. And mistakes, even if you're unhurt, are a costly loss of chemicals. My "make luminol" video cost $2000 to make over the course of a year due to costly "exploration" of the science.
Once I saw that this video was 9 minutes long, I almost clicked away but once I started watching it I thought it was very interesting. NurdRage, I appreciate these videos and I think you do a great job explaining the material.
I think this is a great video; just showed it to a group of first-year university students in a bioanalysis course after teaching the principles of fluorescence spectroscopy.
My friends I'm continuously impressed in the way your videos educate. If I had a child I would with out question use you're videos to help educate him or her
@Ali Jafri When the photon is absorbed, the molecule is excited to a higher vibrational state, as opposed to an electronic excitation. The energy required for excitation to the fist excited state (from the ground state) is discrete, so only one frequency of photon can do this. However, excitations can occur from the ground state to the second excited state, and the third, etc. These would require higher frequency photons. When the molecule relaxes, it does not relax to its initial state (due to it having the highest population), so the light emitted is of a lower frequency.
Don´t quit NurdRage, please try to get permission in the next lab. Learned so much from you and i still want to learn. You and perodicvideos is the best channels out there. Good luck then
I really appreciate the more-thorough explanation of what's happening in this video. It's a bit longer than your other vids, but it's really a nice touch, and this is one of my favorite NurdRage videos so far. Good work, and thanks!
This is freaking huge. It's good to see the physics behind the chemistry. It makes a great deal of sense, and next time I see something with that detached blue-ish glow, I'm going to hit it with UV to see if it's florescence or if I just need to get my eyes checked again.
This might be your best video so far, judging by my preferences. It's quite informative. It goes beyond standard UA-cam sci-videos, but remains in the safe zone of complexity so the amount of people capable of understanding it is not heavily reduced. Now if someone could make a similarly quirky video which extends into the procedures used in molecular biology (fluorescent DNA probes), that would be awesome. There are few videos about it, but they're too dull. Hope you have great holidays.
This is by far the best video you have ever made. Your other videos are by no means bad, they are great! I'm just saying that this one is exceptionally good. I find the short educational explanations very intriguing! Thank you NurdRage! You are one of the reasons that made me choose to study chemistry at the university.
While the dye is the key component, to use it requires special procedures and techniques. But if you want to give it shot there are two techniques i know of: The older technique is Enzyme Linked ImmunoSorbent Assay. The newer technique is Molecular Beacons.
great video. i've been a fan of your videos for a couple of years now and i like how in this particular one you not only introduced some of the theory behind the demo but also some applications.
I'm studying medical lab science and we use spectrofluorometer it works in the same process and this video helped me understanding the results. thank you :)
wow thank you for this. ive always had an interest in flourescence but never really got into how it works. you made everything easy to understand. nice job.
I loved this video. I really like the science-focused detailed explanations. For a lot of your videos I think, "That was awesome, but I'm not sure what it means, or what you can do with it." This really helped to show that and I learned a lot from it. Kudos!
Thanks, Dr. Rage. I've missed your stuff (obviously the chemistry that pays the bills has been a bumper crop of late). WONDERFUL video. Love the disclaimer.
Those 9 minutes went by too fast, I didn't notice it until I read a comment about it. I really enjoyed and learned a couple of new things about fluorescence and I love your videos, they are really enlightening.
My Intro to Organic/Bio Chem and Gen. Bio instructor lectures like this. Seriously. After a nine minute tsunami similar to this, she's like, "Any questions?" And we're on to the next topic. Cheers dude.
.... and once again, for those special people that have lost their hearing to some degree, including you, i have made subtitles. Just click the "CC" icon in the youtube player bar. Don't complain about a problem if you refuse to use an obvious solution. If you choose not to understand me, i cannot help you to.
Indeed, this was a GREAT video. Complete, with diagrams and lots of interesting stuff with fluorescence, i really loved it. But i do remember NurdRage saying he already tryed to make a video with periodic videos and they declined.
This was best educational chemistry video I ever saw :D I understood many things that didn't got in normal school lessons Thank you NurdRage! Looking forward for more awesome video lessons like this one c:
I work in a waste water laboratory where we run a test daily on particular effluent samples for E. coli. We add a compound called Colilert to a sample of treated water and incubate in a special tray at a certain temperature for 24 hours. After incubation, we place the tray under a UV light, and the wells that fluoresce contain E. coli! So it's not just medical, it's also used for environmental applications.
This one is in a class of its own. like magicgonza said - flawless. NurdRage, these newer vids of yours are so so slick - professional even. I LOVED the diagrams. This one displayed a huge amount of your work - the products of so many different little projects u have done along the way. I know yr style is different, but I for one would love to see a collab by u and Brady(Periodic Videos, for anyone who doesnt know already) I love collabs between my UA-cam heroes. merry xmas. best regards, J
I was actually able to pick out the one with the microgram... but I wasn't entirely sure until you shined the light. This video is very helpful. I've been working with painting miniatures with florescent paint but wanted to know more of the specifics in order to use then better.
Thank you Mr. Rage (or is it Dr. Rage?). I love your videos. You make me feel like I'm sitting back in science class. I hope to see many more videos from you in the future.
very well done, thank you for what you do, even when some of the science goes over my head i still feel as if i got something from your videos, and i felt as if i needed to say thank you.
Fascinating. Nice indepth video too. Culd you maybe do a video on Thermochromic materials/inks? I've always been mystified, ever since a colour changing toothbrush I had as a kid. It would be great to know exactly what's going on with the chemistry (if anything, or if it's all just physical).
wow. everything was great in this video. good joke. Lots of information put in a really easy to understand format. A great balance of theory and experiment. Your best work yet.
best fluorescence explanation i've come across. can you show how to make the dye for thermoluminescence (EL) ? even a low-efficiency version will be great. thanks!
Wow. He clearly put a TON of effort into this video. Make sure to give it a thumbs up everyone. NurdRage, what's your educational background? Mind sharing your degrees with us?
Once again Thanks for your contribution to teaching of the sciences. I was on another you tube channel and you must be very popular because he was joking with changing his voice. Cheers
Cool!! I made my own fluorescence experiments using a glass prism instead of a disolution. I used two lasers: green and purple and I obtained fluorescence in the two cases. I have two videos of this.
I love your videos Always very informative and enjoyable to watch I watch these with my little girls and even do some of the safer experiments with them Thank you and keep it up!
This is a fantastic practical demonstration. Since you're working so much with colour, I have a question. Why is it that magenta, which is a mix of red and blue and not even a pure colour, looks similar to violet, which is on the far end of the visible spectrum? Is this purely done in the brain and why?
Nurdrage, I love your videos and because of them and my great teachers, I'm going to major in chemistry in college. Every time I see a concept in your videos that I've learned about, it just seems so cool. This might have been asked before, but where did you go to college? Chemistry is awesome!
This was amazing. I just had a little epiphany about fluorescence... Now to go make some simulations :) You should do some of these informative videos more often! You're great at them.
95 percent of normal people in the world would watch for 5 seconds and stop. the smart people (me u and others on your channel) find this interesting. and I give u a subscribe.
most of the dyes i bought from sigma aldrich or alfa aesar.
Most of the video used:
9,10-diphenylanthracene
9.10-bis(phenylethynyl)anthracene
rubrene
rhodamine B
tetraphenyl porphyrin
and the special dye at 8:00 is a type of FRET based molecular beacon coded to the DNA of e.coli. you can buy the kit online but the compound itself is propriety so even I don't know what it actually is.
Is there such a thing as a single source of info, and encyclopedia of dyes, that shows all of their absorption and emission Spectra, compatibility ,chemical structure solubility etc...
I would like to transfer animated gifs to t-shirts, the animation achieved by cycling long wave shortwave and RGB illumination..
30 years ago I wanted to do animated flame effects on the hood of my car, but the one UV-c only activated red that I could find that was compatible with clear coat and had longevity was horrendously expensive..
If I can find a more imaginative artist to help me, perhaps a phosphorescent stegographic latent image you might see for a second before your eyes adjust 😜
Best intro video on Fluorescence, HANDS DOWN. This is how every professor should introduce this topic in chemistry courses. This was interesting, simple and concise. Great job Nurdrage!
I love how he says Europium Tetrakis Dibenzoylmethide Triethylammonium like it's no problem to say.
What's the matter _you never had Europium Tetrakis Dibenzoylmethide Triethylammonium before?_
It's delicious.
I drink it with my mountain dew so that when I use the bathroom at night I can see where I'm peeing.
Man, that's the best science video I've seen in my life. 10 years ago and today again.
i am so glad this channel is somehow still going. i used to watch so much of it as a kid and was looking for this video specifically because it always amazes me and is a great demonstration. thank you nurdrage for helping people see the cool side of chemistry and make them want to learn! i'm more into computer science now but chemistry will always be fascinating to me.
"do not look into laser with remaining good eye" definitely a good old laser joke.
You can inject this into your veins right
no
So it's heavily implied that you can look into laser IF you have TWO good eyes.
Well, the consequences will not even nearly be as bad as if you do it with just one good eye :)
NurdRage, I've watched a lot of science videos on youtube, but yours are some of my personal favorites. You cover not only interesting topics but actually teach something in the process. Kudos, man.
it's always very informative when Batman villains explain physics to you
Automotive Technicians also use fluorescent dyes to detect coolant leaks. A small amount of dye in a radiator and a UV light lets you see where coolant is coming from. It's a handy tool to have.
This is one of the coolest videos I've seen in a while. I really appreciate that you explained luminescence mechanism the proper way with energy diagrams without dumbifying it.
Too be honest i don't know. Most amateurs i find get their equipment from ebay. I get mine from professional distributors like VWR. I also get glassware from chemglass. But to be really honest chemistry as a hobby is NOT cheap. Glassware is expensive and breaks easily and often. Chemicals get used up. And mistakes, even if you're unhurt, are a costly loss of chemicals. My "make luminol" video cost $2000 to make over the course of a year due to costly "exploration" of the science.
Will you tell me what dye is used to detect e-coli? Thanks in advance!
Maybe that was quinine
I'm in an chemical instrumentation laboratory and this literally helped me understand what I'm studying. Thanks!
I love your videos but this one was something special! Keep up the awesome work!
Once I saw that this video was 9 minutes long, I almost clicked away but once I started watching it I thought it was very interesting. NurdRage, I appreciate these videos and I think you do a great job explaining the material.
I think this is a great video; just showed it to a group of first-year university students in a bioanalysis course after teaching the principles of fluorescence spectroscopy.
The Jablonski diagram is used in many areas of physics and chemistry.
This was better than any other description of fluorescence I've ever seen.
My friends I'm continuously impressed in the way your videos educate. If I had a child I would with out question use you're videos to help educate him or her
We had glow sticks at an event recently, and I was able to explain how they worked because of you. Thank you.
Thanks. Yes I would like to give it a try. Any more information you can send is helpful. Thanks again!
RIP Grant
@Ali Jafri When the photon is absorbed, the molecule is excited to a higher vibrational state, as opposed to an electronic excitation. The energy required for excitation to the fist excited state (from the ground state) is discrete, so only one frequency of photon can do this. However, excitations can occur from the ground state to the second excited state, and the third, etc. These would require higher frequency photons. When the molecule relaxes, it does not relax to its initial state (due to it having the highest population), so the light emitted is of a lower frequency.
Don´t quit NurdRage, please try to get permission in the next lab. Learned so much from you and i still want to learn.
You and perodicvideos is the best channels out there. Good luck then
I really appreciate the more-thorough explanation of what's happening in this video. It's a bit longer than your other vids, but it's really a nice touch, and this is one of my favorite NurdRage videos so far. Good work, and thanks!
my favourite 3 videos of yours: this one, thescience of glow stick, and the complete guide to hot ice, i really like videos like that
This is freaking huge. It's good to see the physics behind the chemistry. It makes a great deal of sense, and next time I see something with that detached blue-ish glow, I'm going to hit it with UV to see if it's florescence or if I just need to get my eyes checked again.
This might be your best video so far, judging by my preferences. It's quite informative. It goes beyond standard UA-cam sci-videos, but remains in the safe zone of complexity so the amount of people capable of understanding it is not heavily reduced.
Now if someone could make a similarly quirky video which extends into the procedures used in molecular biology (fluorescent DNA probes), that would be awesome. There are few videos about it, but they're too dull.
Hope you have great holidays.
This is better than any text book or class, love it. I like how easy and short this concept is explained. Wow i remember i work
This is by far the best video you have ever made.
Your other videos are by no means bad, they are great! I'm just saying that this one is exceptionally good. I find the short educational explanations very intriguing!
Thank you NurdRage! You are one of the reasons that made me choose to study chemistry at the university.
This is awesome. I recently learnd about fluorescence in physics, but the chemical aspect and its usage is just as impressive.
While the dye is the key component, to use it requires special procedures and techniques. But if you want to give it shot there are two techniques i know of: The older technique is Enzyme Linked ImmunoSorbent Assay. The newer technique is Molecular Beacons.
great video. i've been a fan of your videos for a couple of years now and i like how in this particular one you not only introduced some of the theory behind the demo but also some applications.
I just found this channel... possibly the best channel ever.
I'm studying medical lab science and we use spectrofluorometer it works in the same process and this video helped me understanding the results. thank you :)
That's so cool! I have always found light fascinating, but your experiments make it look even cooler!!
wow thank you for this. ive always had an interest in flourescence but never really got into how it works. you made everything easy to understand. nice job.
I loved this video. I really like the science-focused detailed explanations. For a lot of your videos I think, "That was awesome, but I'm not sure what it means, or what you can do with it." This really helped to show that and I learned a lot from it. Kudos!
This vid deserves so many more views. Presented in an interesting, clear and precise manner, defiantly worth a sub :D
you can, in oleds and such. I didn't include that in this video because i don't have the capability to demonstrate it.
Thanks, Dr. Rage. I've missed your stuff (obviously the chemistry that pays the bills has been a bumper crop of late). WONDERFUL video. Love the disclaimer.
I LOVED this video. Don't know why, but it was surprisingly informative and very easy to grasp. Great job, you have altered my fluorescent paradigm.
Those 9 minutes went by too fast, I didn't notice it until I read a comment about it. I really enjoyed and learned a couple of new things about fluorescence and I love your videos, they are really enlightening.
I really hated my university physical chemistry module but this one is far better than the lecturers!
Videos that explain the theory behind the awesomeness are my favorite kind.
5:38
1) electron in ground state
2)raise electron to excited state
3)??????
4) PROFIT
by the way, good work.
My Intro to Organic/Bio Chem and Gen. Bio instructor lectures like this. Seriously. After a nine minute tsunami similar to this, she's like, "Any questions?" And we're on to the next topic. Cheers dude.
.... and once again, for those special people that have lost their hearing to some degree, including you, i have made subtitles.
Just click the "CC" icon in the youtube player bar.
Don't complain about a problem if you refuse to use an obvious solution. If you choose not to understand me, i cannot help you to.
Indeed, this was a GREAT video. Complete, with diagrams and lots of interesting stuff with fluorescence, i really loved it. But i do remember NurdRage saying he already tryed to make a video with periodic videos and they declined.
Outstanding, clear, and well thought out video. Merry Christmas NurdRage!
Even though I am a Bio major, I love going into chem. its sooo cool. Thanks for all of your investments for our enjoyment :)
This is the best video so far! very exaustive and interesting. I really like this style, keep it up!
I think it makes your videos more memorable. Thanks NurdRage your videos are great.
Certainly one of your best videos. An excellent explanation of the effect.
This was best educational chemistry video I ever saw :D
I understood many things that didn't got in normal school lessons
Thank you NurdRage! Looking forward for more awesome video lessons like this one c:
I work in a waste water laboratory where we run a test daily on particular effluent samples for E. coli. We add a compound called Colilert to a sample of treated water and incubate in a special tray at a certain temperature for 24 hours. After incubation, we place the tray under a UV light, and the wells that fluoresce contain E. coli! So it's not just medical, it's also used for environmental applications.
I love how you tied in every other video you made regarding fluorescence. Excellent video :)
One of your best videos. Very entertaining and educational. Thank you for taking the time to do this.
This one is in a class of its own. like magicgonza said - flawless. NurdRage, these newer vids of yours are so so slick - professional even. I LOVED the diagrams. This one displayed a huge amount of your work - the products of so many different little projects u have done along the way. I know yr style is different, but I for one would love to see a collab by u and Brady(Periodic Videos, for anyone who doesnt know already) I love collabs between my UA-cam heroes. merry xmas. best regards, J
This sir was one of the best videos plus explanation i ever saw!
It's nice to see these types of higher level chemistry demonstration videos on youtube. Your voice sounds a lot like Wes from Geoff's group...
Very informative, and a very broad amount of information presented quickly and clearly. I love it.
I was actually able to pick out the one with the microgram... but I wasn't entirely sure until you shined the light. This video is very helpful. I've been working with painting miniatures with florescent paint but wanted to know more of the specifics in order to use then better.
Please never stop making these videos
This episode was flawless.
A+. Best video on the subject that I've seen so far.
This video was so good, I had to write two comments about how good it was.
I very much enjoyed this video. It really helped me to understand the various other fluorescence videos you have on a better level
One of your best (in my opinion), absolutely mind blowing.
Man that was freaking awesome. Thanks for sharing. I believe that you need to learn something new every day and you did it for me.
Thank you Mr. Rage (or is it Dr. Rage?). I love your videos. You make me feel like I'm sitting back in science class. I hope to see many more videos from you in the future.
lol at the warning in the beginning. this video is well done. loved it.
NR, this is a phenomenal overview of fluorescence. As a fellow chemistry teacher, I salute you! :-)
very well done, thank you for what you do, even when some of the science goes over my head i still feel as if i got something from your videos, and i felt as if i needed to say thank you.
Fascinating. Nice indepth video too. Culd you maybe do a video on Thermochromic materials/inks? I've always been mystified, ever since a colour changing toothbrush I had as a kid. It would be great to know exactly what's going on with the chemistry (if anything, or if it's all just physical).
Thanks Nurd Rage! This video deserves a million likes! Thanks for putting Iin the time
It looks like you put a lot of work into this video. And I must say it is a great video! Keep it up!
I would love a video expanding on this, absolutely amazing.
wow. everything was great in this video. good joke. Lots of information put in a really easy to understand format. A great balance of theory and experiment.
Your best work yet.
best fluorescence explanation i've come across.
can you show how to make the dye for thermoluminescence (EL) ?
even a low-efficiency version will be great. thanks!
I have loved his videos, excellent teaching, not just doing.
I would love to see NurdRage do a silver fulminate video.
Wow. He clearly put a TON of effort into this video. Make sure to give it a thumbs up everyone.
NurdRage, what's your educational background? Mind sharing your degrees with us?
Once again Thanks for your contribution to teaching of the sciences. I was on another you tube channel and you must be very popular because he was joking with changing his voice. Cheers
Cool!! I made my own fluorescence experiments using a glass prism instead of a disolution. I used two lasers: green and purple and I obtained fluorescence in the two cases. I have two videos of this.
If I'm not mistaken, some dyes can be electorally stimulated to fluorese as well, They're used in some dye lasers.
I love your videos
Always very informative and enjoyable to watch
I watch these with my little girls and even do some of the safer experiments with them
Thank you and keep it up!
This is a fantastic practical demonstration. Since you're working so much with colour, I have a question. Why is it that magenta, which is a mix of red and blue and not even a pure colour, looks similar to violet, which is on the far end of the visible spectrum? Is this purely done in the brain and why?
This probably is my favorite clip you ever made. Very interesting!
I think I may have to go into the amazing field of chemistry if I watch any more of these videos....
Thanks nurdrage for this present, before the end of winter id like to see a video about how to make a yellow snowman.
Learning about fluorescence on christmas day. Awesome!
I really wish I had you as a Chem teacher while I was in school.
I can't believe I actually understood that... great job!
This video was PERFECT! I only wish you uploaded more often
Nurdrage, I love your videos and because of them and my great teachers, I'm going to major in chemistry in college. Every time I see a concept in your videos that I've learned about, it just seems so cool.
This might have been asked before, but where did you go to college?
Chemistry is awesome!
Smash Glow Crystals is still the coolest vid I've seen. Still though, light has always been the most interesting subject in physics.
I wonder why your vidoes don't have trillions of views. Every human on earth should watch each one of them at least 1000 times u_u
This was amazing. I just had a little epiphany about fluorescence... Now to go make some simulations :) You should do some of these informative videos more often! You're great at them.
95 percent of normal people in the world would watch for 5 seconds and stop. the smart people (me u and others on your channel) find this interesting. and I give u a subscribe.
Lol I was alt tabbed when I heard the "warning intro". Had to switch back and instantly hit subscribe after the "remaining good eye" joke 🤣
@2:31 " This is a sample of Europium Tetrakis (Dibenzoylemthide) Triethylammonium" ... damn. I had to turn on CC just for that.