Sterilization Techniques = Chemical Methods of Sterilization | Chemicals Sterilization Examples

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Sterilization is an absolute term in which there is complete removal of all kind of microorganism from the given object.
Sterilization (or sterilisation) refers to any process that eliminates, removes, kills, or deactivates all forms of life and other biological agents such as fungi, bacteria, viruses, spore forms, prions, unicellular eukaryotic organisms such as Plasmodium, etc.
There is some limitation in heat sterilization like- these are not suitable for those objects which are heat sensitive and may get destroyed in the presence of heat, so to overcome such limitation we use the chemical method of sterilization. chemical methods of sterilization include the use of following chemicals and here we have also included their mode of action.
1. Alcohols dehydrate cells, disrupt membranes and cause coagulation of protein. Examples: Ethyl alcohol, isopropyl alcohol and methyl alcohol
2. ALDEHYDES: Mode of action: Acts through alkylation of amino-, carboxyl- or hydroxyl group, and probably damages nucleic acids. It kills all microorganisms, including spores. Examples: Formaldehyde, Glutaraldehyde
3. Phenol act by disruption of membranes, precipitation of proteins and inactivation of enzymes. Examples: 5% phenol, 1-5% Cresol, 5% Lysol (a saponified cresol), hexachlorophene, chlorhexidine, chloroxylenol (Dettol)
4. Halogen is oxidizing agents and causes damage by oxidation of essential sulfhydryl groups of enzymes. Chlorine reacts with water to form hypochlorous acid, which is microbicidal. Examples: Chlorine compounds (chlorine, bleach, hypochlorite) and iodine compounds (tincture iodine,iodophores)
5. Heavy Metals act by precipitation of proteins and oxidation of sulfhydryl groups. They are bacteriostatic. Examples: Mercuric chloride, silver nitrate, copper sulfate, organic mercury salts (e.g., mercurochrome, Merthiolate)

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Ethylene oxide gas and sterilisation process Eo gas please one more next video sir

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Sir tell me more information about the sterilization like as how do change chemical indicator in autoclave and eto machine about this process please

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Dear sir,Can some disfectants be used in steamers also,kndly reply ,thnx

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Jyoti Rathi thank you. Ha wo rah gaya tha. Micro ke ye video hamne kuch students ke req ke hisab se banaya tha.

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Can you guide me which chemical is more suitable to kill microbes in soil, in addition, the fungicide should remian live,
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Sir please upload more videos of sterilization process

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Khushboo Mariyam Respected madam. We have uploaded few videos of heamatology (Theory and Practicals) please find in our channel.

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It is also known as cold sterilization or not ?

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Sir instrument ko sterilizie karne se pehle kis chemical se wash karse tn ki patient ko infection na ho baad mein

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Autoclave se kia jaata hai instruments ko

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@@preetichoudhary8557 aise object ke liye jo heat sensitive hote hain

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Sterilization will remove 100% mo from non living objects and depyro. Will just kill pyrogens

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sir radiation or vibration methods

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Neha Mahto Mahto Actually we are from pharmaology. These 3 videos of MB was made as per request of student at exam time. We will conti. Micro once cology will over.

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Which chemical attracted by other chemical.
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Already provided. Please check the description box

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Cold staerlization also known as
By radiation
By chemicles

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Basically both

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Radiation methods

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