Cell Cycle Analysis By Flow Cytometry

Flow cytometry is one of the most important techniques to understand different aspects of cell biology, stem cell biology, cancer biology, and immunology. One of the interesting applications of flow cytometry is understanding the cell cycle based on DNA content.
In this lecture first, we will discuss the basic principle of flow cytometry and then will utilize the fundamentals to analyze the cell cycle and how we calculate the proportion of cells present in various phases of the cell cycle. This lecture will not only explain the principle but also discusses the analysis of graphs and data.
The whole discussion includes the following -
1. Fundamental principle of flow cytometry.
2. Looking at the cell cycle from the point of view of DNA content.
3. How does the flow cytometer use DNA content to understand the cell cycle.
4. Graph of the cell cycle in flow cytometry.
5. A brief idea about different mathematical models to understand the graphs of the cell cycle.
6. Cell cycle inhibitory drugs and their graphs.
7. The brief protocols and important points of the protocol.
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[@sadiqamin3023]

Excellent 👌

[@juliuskoome3055]

Thanks Sir. You made me understand the principle of flow cytometry better. Thanks.

[@shreyasgaikwad5108]

Absolute excellence!! Hats off to his dedication towards educating students.

[@Bodhee_Prep]

Very well explained:)

[@deborahudeh196]

Thank you Sir. This video just explained my result that I have been trying to understand for months.

[@charlesidu-okojokwu9732]

Greatly appreciated sir. Keep the good work on 👍

[@giddy5611]

wow wow wow wow wow wow man thank you i was struggling to understand this for months i gave up but the way you explained so easy to understand

[@priyamchatterjee408]

nice video Nishant sir!!! Want more of these

[@nikhilkulkarni7388]

Very well explained sir...
These videos are helping me a lot...
Thank you so much...

[@karimbarakat3681]

Best explanation ever ❤

[@basyalpalpa5680]

worldclass..hats up sir

[@yadagirisubhashni3672]

thank you very much sir ,can you please tell me ROS quantification

[@PoojaSingh-ft2wk]

Efforts Appreciate….. keep doing 👍

[@furqanmemon7693]

Great explanation

[@travelkuchpal2188]

well explained thank you sir....

[@khalidakram]

Very good video. Very relevant info, and superbly explained. Why are you guys not doing more videos??????

[@AsadAli-qm3wx]

Excellent

[@jskim9978]

Thank you!!

[@foodmojobyneha]

Cell cycle graph was explained very nicely with the principle of the flow cytometer, waiting for your next lecture, If possible please explain other significances of 3 laser flow cytometer thankyou so much sir

[@arkadeep-ark9805]

Nishant sir OP

[@parsadar8219]

hi very helpful videos how can somebody contacts you in person email id? Can you make videos on PCR and ELISA as well and using immunoflorecense microscopy

[@nammub9400]

Sir, may I know what your email address is ?

[@nammub9400]

I have some doubts