tks for the tutorial. what about the setting ? should we save the setting the first day and reuse those or use the new cytek setting that change every day with the new QC ?
Thank you for the series of helpful videos! Is there any way to adjust the gating on the references controls after they have been stored in the library? I have found my samples to be very heterogeneous (also stain multiple different tissues which require unique unmixing strategies) and I usually have to adjust the gates on the positive and negative peaks of some markers multiple times before I end up with an acceptable unmixing.
tks for the tutorial. what about the setting ? should we save the setting the first day and reuse those or use the new cytek setting that change every day with the new QC ?
Thank you for the series of helpful videos! Is there any way to adjust the gating on the references controls after they have been stored in the library? I have found my samples to be very heterogeneous (also stain multiple different tissues which require unique unmixing strategies) and I usually have to adjust the gates on the positive and negative peaks of some markers multiple times before I end up with an acceptable unmixing.