Mass spectrometry|Mass spectroscopy|Mass spectrometer|Principle Instrumentation,working,applications

Mass spectrometry video describes the principle, instrumentation and working of it. It also discusses the interpretation of mass spectrum with an example. It enumerates the advantages, disadvantages and applications of mass spectrometry.
You will be able to state the principle of mass spectrometry explain the instrumentation in detail and working of mass spectrometry discuss the mass spectrum with an example list out the applications of mass spectrometry.
Principle of Mass Spectrometry
Mass Spectrometry is a technique used to identify the compounds present in the sample by vapourizing the sample into mixture of gaseous ions (with or without fragmentation), sorting/separating the deflecting gaseous ions in the presence of magnetic field based on their mass to charge ratio (m/z) and recording the relative abundance of each ion.
Instrumentation & working of Mass spectrometer
Inlet system - The sample is introduced and vapourized by heating. Ionisation source - Sample molecules or atoms are ionised by knocking out one or more electrons (forms mixture of gaseous ions i.e molecular ions and fragmented ions). Electron bombardment - Electrons generated in the glowing filament bombard with the sample molecules and produces beam of ions. Thermal ionization - Atoms get ionized when in contact with heated metal surface (filaments such as Pt, W, Re, Ta etc.) Direct current arc - Plasma discharge (ionized gas with positive ions and electrons is produced when two electrodes are placed perpendicular to the axis of electric field with a high potential difference (few hundred volts) Accelerator - The ions are accelerated and focused as a single beam by passing through accelerating slits of higher potential (~10000 volts) to lower potential (0 V).
Mass analyzer/Separator - The ions are sorted and separated based on mass to charge ratio (m/z)
Quadrupole - separation based on combination of radio waves and DC electric fields
Magnetic sector - uses magnetic field to deflect the ions based on mass. The lighter ions (low m/z ratio) are deflected more than the heavier ions (high m/z ratio) The high charged ions are deflected more than the low charged ions
Ion detector - Electron multiplier, scintillation counter, faraday cup detector Detects the number of ions entering the detector and produces electric current proportional to it
Recorder It records the relative abundance of each ion having specific mass/charge ratio (m/z) to give a mass spectrum
Advantages of mass spectrometry
Quick analysis.
Highly reproducible.
Can determine up to 16-76 components in one stream.
Disadvantages of mass spectrometry
Distinction of compounds having same m/z ratio is difficult.
Distinction of isomers of the compound having same m/z ratio is difficult.
Applications of mass spectrometry
Determination of the molecular weight of compounds.
Determination of the molecular structure from the mass spectral data and fragmentation pattern.
Determination of the molecular formula.
Distinguishes different isotopes of a given element.
Analysis of proteins, peptides, lipids and oligonucleotides.

[@poojapanurkar5640]

Thank u ma'am for explaning in simple way🙏😊

[@RevathiPurushothaman]

It's my pleasure.

[@ramuramkorram4605]

​ o99oo9o❤

[@hinaprakash_]

I love all your lectures ma'am.. makes the topic so much easier to understand...I love all the schematics ... :)

[@RevathiPurushothaman]

Thank you so much. Pleasure to hear. Thanks for your constant support.

[@byrisettineelima7351]

Extraordinary and excellent presentation madam... It's really such a valuable information and vedio mam.. Thank you so much for such information... It's very helpful for my exam madam.. Please keep on doing such vedios madam

[@RevathiPurushothaman]

Thank you so much. Its my pleasure.. Sure, I will continue.

[@balamurganp7224]

❤ amazing lecture mam! ❤🎉

[@RevathiPurushothaman]

Thank you so much.

[@elizabethprah6570]

Wow
U r the best chemist

[@RevathiPurushothaman]

Thank you very much. This motivates me.

[@yasinbangash2250]

Amazing ♥️

[@RevathiPurushothaman]

Thank you so much.

[@hosabettumohan8041]

Good,simple explanation

[@RevathiPurushothaman]

Thank you.

[@vijayanisy3538]

Nice explanation thanq mam

[@RevathiPurushothaman]

Thank you.

[@qazi__chemist4300]

Base peak is not always be a molecular peak.its depends upon the aboundance of particular fregment.

[@RevathiPurushothaman]

Sure. You are correct. I just took one example to explain.

[@vivekjamadar9398]

Awesome mam , are you lecture

[@RevathiPurushothaman]

Yeah!

[@SG73088]

Nice lecture madam

[@RevathiPurushothaman]

Thank you madam.

[@gulfishashaikh4473]

Ma'am plz make the video on INDUCTIVELY COUPLED PLASMA -MASS SPECTROMETRY.

[@RevathiPurushothaman]

Sure

[@anjalidhiman9911]

mam please do video on NMR spectroscopy please..
Friday my exam final ...

[@sadatibinabdu1030]

Excellent presentation ,can i get those ppt slides and the full notes

[@RevathiPurushothaman]

Thank you. Check my blogspot.

[@byrisettineelima7351]

Madam please do vedio on Nmr spectroscopy... Please madam

[@RevathiPurushothaman]

It would be my next video.

[@rusheenaaditi2790]

mam can you make a video of centrifugation

[@RevathiPurushothaman]

Sure.

[@shwetasubramaniam2059]

mam please share notes for ionization sources

[@kennyiichixo]

Mam pls explain nmr spectroscopy

[@RevathiPurushothaman]

Sure. Will do it shortly.

[@sadatibinabdu1030]

Please I am waiting for your response

[@RevathiPurushothaman]

What is your query?

[@yasinbangash2250]

Amazing ♥️

[@RevathiPurushothaman]

Thank you so much.