Cell & Tissue Culture [Part 2]: Common Mistakes

Science and superstition don’t typically mix, but you find a surprising amount of overlap in these ideas when scientists work on cell and tissue culture. Contamination can happen for a whole host of reasons, and months of work can be lost overnight if a single flask is contaminated. In this video we will show you a series of demonstrations of cell culture experiments, each of which is flawed for different reasons. Being able to pre-visualise the mistake before you make it is an important part of learning these techniques
My name is Jack Wang, a microbiologist and science educator, and the 2020 Australian University Teacher of the Year. You can find out more about my work here: jackwang.com.au
The blog post accompanying this video: jackwang.com.au/blog/findtheerror
This is part 2 of our video series on cell culture - you can find other videos in the series below:
Part 1 The Basics: • Cell & Tissue Culture ...
Part 3 Bacterial Culture and Cell counting: • Cell & Tissue Culture ...
Aseptic Technique
Infection Control: • How Infection Control ...
#biolabcollective #microbiology #tissueculture #scienceexperiment
The equipment I use to make these videos. I will earn a small commission if you purchase using these Amazon Affiliate links, which will be re-invested back into the channel.
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00:00 Introduction
00:38 Key Tissue Culture Principles
02:05 Scenario 1 - Pipetting Media
03:07 Scenario 2 - Resuspending pelleted cells
04:06 Scenario 3 - Media Change
05:41 Scenario 4 - Working with 24-well plates
07:16 Conclusion

[@funny11744]

thanks for sharing your knowledge.

[@BioLabCollective]

Thanks for watching!

[@LNN1356]

Watching this gives me anxiety, especially the resuspending cells part.

[@BioLabCollective]

Shooting and editing the footage was no cakewalk either - went against all of our instincts and training!