Yes, the RNA following elution goes through quality testing including Nanodrop and Bioanalyzer and I only use RNA if it satisfies all these. Hope that hlps. Thanks.
@@innovative_learner Thank you for your answer. Do you blank the nanodrop with the nuclease free water from the kit? If so what is the 260/230 ration like? There must be a certain amount of carry over from the last well the plunger is in, into the elution tube with H20 in it. I wondered if this might be deliberate to create a buffered solution in the elution tube.
The kits cartridges help purify samples using paramagnetic particles, which provide a mobile solid phase to optimize sample capture, washing and purification of nucleic acid. Maxwell Instruments are magnetic particle-handling instruments that efficiently bind nucleic acids to the paramagnetic particle in the first well of a prefilled cartridge. The samples are processed through a series of washes before nucleic acids are eluted.
Thank you ! Now I know
Thanks for your video. Do you nanodrop these eluates? Have you noted any reagent carry over?
Yes, the RNA following elution goes through quality testing including Nanodrop and Bioanalyzer and I only use RNA if it satisfies all these. Hope that hlps. Thanks.
@@innovative_learner Thank you for your answer. Do you blank the nanodrop with the nuclease free water from the kit? If so what is the 260/230 ration like? There must be a certain amount of carry over from the last well the plunger is in, into the elution tube with H20 in it. I wondered if this might be deliberate to create a buffered solution in the elution tube.
Do you know what components have each tube of the cartridge?? And which is the function of the Plunger?
The kits cartridges help purify samples using paramagnetic particles, which provide a mobile solid phase to optimize sample capture, washing and purification of nucleic acid. Maxwell Instruments are magnetic particle-handling instruments that efficiently bind nucleic acids to the paramagnetic particle in the first well of a prefilled cartridge. The samples are processed through a series of washes
before nucleic acids are eluted.
@@innovative_learner thanks!!
Is there a special reason for mounting cartridges below 16 in the center?
No, there is no specific reason for this. You can insert cartridge anywhere you like. Hope that clarifies. Thank you.
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