That's actually half the truth. Through a process called NETosis, the nutrophil can survive the process. I guess the choice depends on the situation. I qoute - "Vital NETosis can be stimulated by bacterial lipopolysaccharide (LPS), other "bacterial products, TLR4-activated platelets, or complement proteins in tandem with TLR2 ligands. Vital NETosis is made possible through the blebbing of the nucleus, resulting in a DNA-filled vesicle that is exocytosed and leaves the plasma membrane intact. Its rapid formation and release does not result in neutrophil death, however, the cell is without DNA, raising questions about whether a cell without DNA can be considered alive. It has been noted that neutrophils can continue to phagocytose and kill microbes after vital NETosis, highlighting the neutrophil's anti-microbial versatility." - From Wikipedia
Hello! I would be careful with using the word "truth" as experts in the field still have not been convinced and do not have a defined unified view. Check: doi:10.1111/cmi.12652 and doi:10.1038/s41418-018-0261-x
Excellent video! What antibody did you use to detect the extracellular DNA? And what was it labeled with?
Good Question!
Can you tell the procesure of SEM
Thank you for the video Ryan!! (NETs!!!!!!!)
What is the experiment name
That's actually half the truth. Through a process called NETosis, the nutrophil can survive the process. I guess the choice depends on the situation. I qoute - "Vital NETosis can be stimulated by bacterial lipopolysaccharide (LPS), other "bacterial products, TLR4-activated platelets, or complement proteins in tandem with TLR2 ligands. Vital NETosis is made possible through the blebbing of the nucleus, resulting in a DNA-filled vesicle that is exocytosed and leaves the plasma membrane intact. Its rapid formation and release does not result in neutrophil death, however, the cell is without DNA, raising questions about whether a cell without DNA can be considered alive. It has been noted that neutrophils can continue to phagocytose and kill microbes after vital NETosis, highlighting the neutrophil's anti-microbial versatility." - From Wikipedia
Interesting concept. I'm usually wary of relying on Wikipedia though.
Hello! I would be careful with using the word "truth" as experts in the field still have not been convinced and do not have a defined unified view. Check: doi:10.1111/cmi.12652 and doi:10.1038/s41418-018-0261-x
@@HicamorHaecpatriaest they provide references for the claims..
Yes a cell without nucleus may be considered alive, until they have intact cell membrane and ATP ! For example, RBCs.
It may be able to act without dna, but not for long.
THAT WAS FUCKING AWESOME!
SOOO COOL 🎉🎉
This mechanism is depends on Platelets activation first :)
Badass cells
spiderman