Wound Healing Assays Using the Culture-Insert 2 Well

Practical demonstration of how to use the Culture-Insert 2 Well for wound healing assays and video microscopy.
Find out how to use the ibidi Culture-Insert 2 Well as a complete solution for wound healing assays. Just a few steps are required to get from sample preparation to image analysis.
Product link:
Culture-Insert 2 Well in µ-Dish 35 mm: ibidi.com/cult...
Visit our Wound Healing and Migration application site:
ibidi.com/cont...
Download our Application Guide "Wound Healing and Migration Assays": ibidi.com/img/...
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[@peterduplessis9649]

How many cells (average) was placed in the 70uL cell suspension? Also, could you add the protocol in the link please.

[@ibidiGmbH]

Hi Peter, thanks for asking. In average, 21,000 cells were in the 70 mL cell suspension (= 3 x 10^5 cells/mL).
Please find the link to the Instructions here: ibidi.com/img/cms/products/labware/open_removable/E_8XXXX_CI_2Well/IN_8XXXX_CultureInsert_2Well.pdf
A detailed protocol for a wound healing assay using MCF7 cells can be found here: ibidi.com/img/cms/support/AN/AN21_Wound_Healing_Assay.pdf
Hope this helps!

[@setiabudi1596]

how to measure the percentage ? let me know

[@ibidiGmbH]

Hi Setia, thanks for your question. How to quantify these assays can be found in our Application Note AN21: ibidi.com/img/cms/support/AN/AN21_Wound_Healing_Assay.pdf

[@setiabudi1596]

@@ibidiGmbH how to get the software ?