Wound Healing Assays Using the Culture-Insert 2 Well
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- Опубліковано 8 вер 2024
- Practical demonstration of how to use the Culture-Insert 2 Well for wound healing assays and video microscopy.
Find out how to use the ibidi Culture-Insert 2 Well as a complete solution for wound healing assays. Just a few steps are required to get from sample preparation to image analysis.
Product link:
Culture-Insert 2 Well in µ-Dish 35 mm: ibidi.com/cult...
Visit our Wound Healing and Migration application site:
ibidi.com/cont...
Download our Application Guide "Wound Healing and Migration Assays": ibidi.com/img/...
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How many cells (average) was placed in the 70uL cell suspension? Also, could you add the protocol in the link please.
Hi Peter, thanks for asking. In average, 21,000 cells were in the 70 mL cell suspension (= 3 x 10^5 cells/mL).
Please find the link to the Instructions here: ibidi.com/img/cms/products/labware/open_removable/E_8XXXX_CI_2Well/IN_8XXXX_CultureInsert_2Well.pdf
A detailed protocol for a wound healing assay using MCF7 cells can be found here: ibidi.com/img/cms/support/AN/AN21_Wound_Healing_Assay.pdf
Hope this helps!
how to measure the percentage ? let me know
Hi Setia, thanks for your question. How to quantify these assays can be found in our Application Note AN21: ibidi.com/img/cms/support/AN/AN21_Wound_Healing_Assay.pdf
@@ibidiGmbH how to get the software ?