Hello sir, ap bahut acche se samjhate ho.. clearly samaj ata h...mera ek question tha theoretical plates ka product par kya effect hota suppose theoretical plates require h 2000 but acheive ho rhi h 1500 but peak shape good h...
Theatrical plate has dominant role on product peak shape and sharpness...and peak shape is highly keep impact on product accurate results....and if theatrical plates are 1500 and initial was 2000 . So it shows column working very good.. Keep in mind and make sop regarding this .. always obselete the column when theatrical plates becomes half of the initial... It's best practices
@@rohitmanapure4776 cannot be increased.. and can be maintained proper washing procedure.. Theoretical plates depends on column to column by manufacturer
Sir how we can check the remaining number of theoretical plates? How we can check that the remaining theoretical plates are 50% or 25% from original value?
Jab aoke pas initial theoretical plats hoti Hain and jab last Jo aoke pas aati Hain unse ap % calculate kar laitai hain very simple..for example Initial thee 1800 and abi last analysis wali are 900. To %= 900x100/1800
Good explanation sir
Hello sir, ap bahut acche se samjhate ho.. clearly samaj ata h...mera ek question tha theoretical plates ka product par kya effect hota suppose theoretical plates require h 2000 but acheive ho rhi h 1500 but peak shape good h...
Theatrical plate has dominant role on product peak shape and sharpness...and peak shape is highly keep impact on product accurate results....and if theatrical plates are 1500 and initial was 2000 . So it shows column working very good..
Keep in mind and make sop regarding this .. always obselete the column when theatrical plates becomes half of the initial...
It's best practices
how to increases the therotical plate
@@rohitmanapure4776 cannot be increased.. and can be maintained proper washing procedure..
Theoretical plates depends on column to column by manufacturer
Hplc interview questions pe video banayiye
Sir how we can check the remaining number of theoretical plates? How we can check that the remaining theoretical plates are 50% or 25% from original value?
During analysis system calculate through software and gives you on SST report...
You can compare it by the initial first analysis
Jab aoke pas initial theoretical plats hoti Hain and jab last Jo aoke pas aati Hain unse ap % calculate kar laitai hain very simple..for example
Initial thee 1800 and abi last analysis wali are 900. To
%= 900x100/1800
Please make video on needle wash
Sure
How to hplc flush method
Which solvent use flush hplc??
Video uploaded already about it but no matter again will make
Sir uv pe bhi video banaiye
Sure...just keep watching and sharing