How to design pcr primers using NCBI primer blast

This is practical tutorial for pcr primer designing by NCBI primer blast.

[@ghanishta5986]

Very helpful and informative video sir .. thanku very much

[@pradeepkumarn9331]

I hope this is more useful for the primer design. But if possible can u elaborate the information regarding the range and information about the GC content, self complimentary!!! It will be more helpful
Thankyou very much

[@debabanighosh4950]

Thanks a lot for your wonderful videos. I have a request for videos on metagenomics data analysis in details, machine learning, R and Python.
Thanks.

[@priyamishra9893]

Thank you sir for this video, very helpful 🙏

[@syedazahidaali9442]

A very good way for understanding.thanks

[@bornoporichoi6751]

My primers shows unintended similarity with other Genes. How to get rid off and get primer specific to gene???

[@GokmenTortuk]

Thank you sir stajdan atıldım :))

[@pervinayrancioglu8044]

alo desam mi gokmeni geri alin

[@abubakarraja7605]

why you have not selected broad green line portion for designing primer?

[@cutiepie-uu5sl]

thanks alot it really helps me alot

[@philominalpathinathan9635]

Nice explanation sir thank you🎉

[@drneerajkumar]

Thanks for your feedback

[@user-cs4uf2nc4h]

Very informative

[@ammarahmehak9986]

Sir make detail videos on ARMS and RfLp pcr primer design with detailed explanation

[@Universe12355]

Love your channel and the way you make difficult concepts easy.
I had a question about primer property checking using Primer-BLAST.
I have a primer that contains degeneracy/wobbles and I want to check its properties such as Tm, self-complementarity, self 3’ complementary using the primer- blast…
However, when I use the primer Blast and search, it states that “ambiguity letters other than N are not allowed in the primer sequence”
So that forced me to replace all the degenerate/wobble letters with N.
Would that still make the property results correct? Am I still getting the right answers if they are replaced with N?
Thank you so much, I would really appreciate some insight on this, as I can not find an answer anywhere.

[@farahnaz7869]

After selecting F primer, when i press Control to select R primer , the F selection is deselected. please guide

[@ravidubey347]

press control and dont leave the button... while pressing it select the R primer and then leave the button

[@deepanshisingh11]

​@@ravidubey347it still isnt working. Same issue

[@halimaalamgirusha3986]

thank you sir

[@anuradhapatil1114]

How to validate the MSP Primers before ordering them and can we do the 5 gene methylation in a single tube? can anyone explain about how to carry out?

[@TahiraAliAwan-ww9cd]

How to download the designed primer

[@samarsameer6031]

Very informative