This is great! Hey David, I was inspired by one of your videos to make a bioreactor for my internship project. I'm going to post a video about it and it'd be cool if you saw it. It'll be up in a few days. Thank you for continuing to make awesome content and being an inspiration to all of us!
1:57 In your expensive setup, how can you get a two color image? What kind of emitter/excitation filter and light was used here? In your DIY, you have a blue (445nm?) flashlight and yellow (blue filter). So... should we simply understand that the diy yellow (blue filter) will let the green and orange light passing thru and therefore we can also get theses amazing 2 color image on the cheap? Another question... Could we just replace the white light with a 1W or 3W blue light from Ebay? 450-445nm or 365nm or 395nm single led. What would be useful/useless for fluorescence microscopy? I also saw many high quality filter mirror of different wavelength (1cmX1cm for under 2$). Thank you for sharing your expertise on UA-cam. Alex from Montréal
@@alexandrevaliquette3883 I’m not sure the wavelength of the blue flashlight. Yes you can see GFP and RFP just fine with the DIY setup so yes you can get two color fluorescent microscopy on the cheap. As for the second question maybe so I haven’t tried it though. The wavelength of the excitation light depends on filter depending on what you’re looking at. Different fluorescence proteins and compounds like DAPI for staining can all be a bit different.
awesome! just a question about the filter - you mentioned it was a UV filter, but it also blocks visible blue light? are most UV filters designed to block blue light in addition to UV?
Hi David, i have one question. I want to be "biohacker" like you, so my question is. Should I go to the University and first learn all the theory, or make a lab in garage and work like you? My goal is to make human live longer, for example by incapacitate one protein (ACMSD) which is blocking process of NAD+.
This is great! Hey David, I was inspired by one of your videos to make a bioreactor for my internship project. I'm going to post a video about it and it'd be cool if you saw it. It'll be up in a few days.
Thank you for continuing to make awesome content and being an inspiration to all of us!
Колись використовував фільтр для Місяця на окуляр (фільтр телескопа)
Зараз маю насадку ои-17 і набір ои-18 для освітлення з фільтрами
Thats very cool! I can't wait to see how it contiunes in new generations.
great little find
Love this hack! It saves thousands. Can you link the flashlight and film pls?
Amazing, thanks for sharing
1:57 In your expensive setup, how can you get a two color image? What kind of emitter/excitation filter and light was used here?
In your DIY, you have a blue (445nm?) flashlight and yellow (blue filter).
So... should we simply understand that the diy yellow (blue filter) will let the green and orange light passing thru and therefore we can also get theses amazing 2 color image on the cheap?
Another question...
Could we just replace the white light with a 1W or 3W blue light from Ebay?
450-445nm or 365nm or 395nm single led. What would be useful/useless for fluorescence microscopy?
I also saw many high quality filter mirror of different wavelength (1cmX1cm for under 2$).
Thank you for sharing your expertise on UA-cam.
Alex from Montréal
@@alexandrevaliquette3883 I’m not sure the wavelength of the blue flashlight.
Yes you can see GFP and RFP just fine with the DIY setup so yes you can get two color fluorescent microscopy on the cheap.
As for the second question maybe so I haven’t tried it though. The wavelength of the excitation light depends on filter depending on what you’re looking at. Different fluorescence proteins and compounds like DAPI for staining can all be a bit different.
awesome! just a question about the filter - you mentioned it was a UV filter, but it also blocks visible blue light? are most UV filters designed to block blue light in addition to UV?
Hi David, i have one question.
I want to be "biohacker" like you, so my question is.
Should I go to the University and first learn all the theory, or make a lab in garage and work like you?
My goal is to make human live longer, for example by incapacitate one protein (ACMSD) which is blocking process of NAD+.
Why you wouldn’t want to learn all the theory behind it from actual people who is working on it? Do both
Brilliant, also thanks.
Do you have an email I can reach you at ?
You can email me at dishee@midgardgenetics.com