Detect and remove doublets in R (scRNAseq)

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  • Опубліковано 7 лют 2025
  • In this tutorial I will explain how to remove doublets from scRNAseq data in R using R package after running one or more doublet detection tools. For this tutorial, I’ll be using RStudio, and you’ll need the tidyverse packages as well as Seurat.
    You will learn how to:
    get doublet/singlet count and percentage for one or more doublet detection tools
    -get doublet vs singlets stats
    -remove high-confidence doublets from your Seurat object
    and more!
    And as always, you can find the code I am using in this tutorial at biostatsquid.com, where you can also find a step by step explanation of the code. For this tutorial you will need R, or Rstudio, and you will need to install the package listed above.
    Hope you like it!
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КОМЕНТАРІ • 2

  • @alicjas7554
    @alicjas7554 13 днів тому

    You make very helpful videos! Thanks :)

  • @biostatsquid
    @biostatsquid  5 місяців тому

    Check out the blogpost & code here: biostatsquid.com/remove-doublets-scrnaseq/