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I notice that I still have spores left over in my syringe. What’s the best way to use those spore? Should I refill it with distilled water? Thoughts guys?
Thanks that’s exactly what I needed to hear. I once melted 5 syringes into the bottom of my pc smelling like burnt plastic I quickly turned it off and when I opened it they had formed one blob which I was able to peel off of the metal.
I love your videos! I just take your syringe apart, rinse off particulate matter then drop in alcohol for 1 hour, reassemble irrigate with distilled water and store for next use in mason jar. Been using this technique for 8 years and have had no problems. I guess more than one way to skin a cat.
@@droidnick No. However it's on a par with reverse osmosis water for ppm bacteria counts. To sterilize place in mason jar and pressure cook for 30 min at 15 psi. Have a port on the lid so you can keep sterile when you need to transfer to another container. You can use 4 jars and make about a gallon quickly.
Im new to the game, but I found putting your used syringes in a mason jar with a lid modified or unmodified in the PC t for 30 minutes. Work perfect. Never had any warped or melted syringe, so far. Been in the game for 1 year.
Depends what it's made out of. Even if the body stands up to the heat the black part won't. It's very succeptable to heat and solvents. The best option is to just use glass syringes and PC them.
Alcohol + boiling water work well. Had no ill effects. Rinse them with alcohol and boiling water a few times. You can alternate from cold to hot a few times to force any bad mold spore cell walls to get destroyed.
Using this method you won’t have to worry much about deformation, and don’t have to be careful about accidentally breaking it. Also much cheaper than a glass syringe.
Take syringe apart put it in boiling water for 5-10mins boom you have a stairalize syringe. Ive never had a syringe melt before maybe you can autoclave them? I feel like most syringes can take boiling water these days
Yeah I was wondering why he is tediously sucking up the boiling water, letting it sit, and pushing it out -- rather than just disassembling the syringe and leaving it inside the boiling water for some time. Seems much, much easier and simpler to do it this way than the method he shows in the video, and I'm all about maximizing efficiency 🤘🏻 I'm also wondering if I can just leave the disassembled syringe to soak in a solution of hypochlorous acid, which is the cleaning solution I use for everything instead of isopropyl alcohol. I'd imagine that would work just as well, and it would take about the same amount of time.
I actually accidentally harpooned my finger trying to get the cap off one of my needles lol 18 guage needle 💉 right through my glove , then the blood came , figured I had better start over lol 😂
Same here. This is the way. Endospores only germinate at certain temps so cold then hot a few times and alcohol are a great way to improve the sterility of the syringes.
If boiling water is good enough for spore syringes, what is the purpose of autoclaving syringes? I've begun to load syringes with 10cc of water, wrap them in foil and sterilize them if there is extra space in the PC. It's quite convenient to have 10cc increments of sterilized water on hand. I've never made MSS syringes before -- do you think I could let some spores drop into a container, and then inject the presterilized water into the container and draw it back up to make a MSS?
There’s no need to autoclave syringes. You can also just pull up 10cc of boiling water as I show in this video after rinsing out your syringes with boiling water a few times (as I have shown in this vid). As for making MSS, yes that is the way to do it, ofc in a SAB or flowhood etc… The best medium I found to mix the water and spores in is a shot glass. With plastic the spores tend to stick, and anything wider than a shot glass becomes a challenge to suck into the syringe.
I’m assuming you would prefer to use distilled water for this? I have hard water, which tends to leave crust at the bottom of my kettle and such…. So yes to distilled water?
A couple weeks ago, before I got my agar, I inoculated a popcorn grain jar with MSS, and I'm thinking I may not have left enough moisture in the kernels before pressure cooking, so I want to reuse a syringe to add more water through the injection port. I've used syringes for BRF in the past without issue, but I know popcorn is more susceptible to contaminants. Is it worth trying to inject more water after cleaning up a syringe? The main reason I'm thinking I didn't leave enough moisture in the kernels is because there was actually water at the bottom of the jars after I sterilized my last couple jars, which the popcorn quickly absorbed after shaking the jar 24 hours later. The first two had noticeable water on the sides of the glass but I didn't check for, or see water at the bottom. Lastly, do they dry up relatively quickly, is maintaining moisture and issue with gas exchange ports? Thank you in advance and I apologize for the long post!
I had some extremely dry grains when I was trying out the flash prep TEK. I ended up shooting 30cc of water each over 2 separate SAB sessions (the first one was not enough). Out of 10 jars or so about 4 contaminated when all was said and done. It’s worth it if it’s not colonizing, you don’t have much to lose at this point.
@Mycophilia great, thank you for the reply!. I took a closer look and saw the tiniest bit of condensation in the jar so decided to check back in another week and see how they're doing. One of them is doing quite well. The other two are pretty slow
Most people who have had babies would know sterilising baby bottles etc in boiling water is centuries old , not sure why so many people complicate sterilising things , not rocket science .
@@Mycophilia Thank you 🌹. I am looking for a quick solution when I do not have time to boil the syringes. So, I thought that it might do the job if they rinsed in bleach and then rinsed in 91% alcohol, and after that, the first sterilized liquid was discarded. Maybe it's a crazy idea🥴?
@@Mycophilia I guess but personally, I would just use new ones. If you can't access them for some reason I understand the purpose of sterilizing and reusing.
Waste of time and energy. I just paid $7 for 100 disposable 10ml Luer lock syringes with needles. With the energy cost and the time it takes to do this, I highly recommend against it. If you just have a syringe or two, also remove the rubber ftom the plunger and just soak it all in a jar of 70+% Etanol, which you can use for cleaning your workspace later and thereby not waste anything.
Ethanol does not sterilize like boiling water does, that is why ethanol is not used. It’s not that much time lol, just boil the syringe for a couple of minutes. Help the environment out a little.
it costs WAAAYYY more to sterilize these syringes than to buy a few brand new ones for a couple of dollars.... Sorry mate, but this "method" is not usefull at ALL !
The secret ingredient to the perfect substrate is a *LIKE* and a *COMMENT* on a Mycophilia video! It's certified! 👏🏼🥂👽
1) Patreon Replacement (memberships for videos and monthly genetics): tinyurl.com/mycosage
2) Mentorship and Genetics, I don't bite!: mycophilia.official@gmail.com
3) Discount codes in description
🍄💜
Sage
😊
Can u prolong fruiting and get more fruits by harvesting some fruits as they appear. Saving your substrate from getting sucked dry 🤷🏽
Absolutely 💯% Undeniable Scientific Proof!!
The "Like Button" phenomena is A Fact!!❤
At the Unseen University, I am conducting Peer Reviewed Scientific Studies of the "Commenting" phenomena now as well!!❤
I notice that I still have spores left over in my syringe. What’s the best way to use those spore? Should I refill it with distilled water? Thoughts guys?
I was wondering about if syringes and needles could be reused. Perfect timing Sage. Thanks🦋
😊
I love this idea. I never really thought about trying to reuse a syringe but it seems pretty simple. Definitely worth it for someone on a budget.
Very helpful I’m sterilizing a syringe right now for my first agar slurry!
Thanks for this great advice! Especially enjoyed the tip about gathering sterile water at the end
Thanks that’s exactly what I needed to hear. I once melted 5 syringes into the bottom of my pc smelling like burnt plastic I quickly turned it off and when I opened it they had formed one blob which I was able to peel off of the metal.
Just in time. This Info i need right now
I impulse-seal mine in an autoclave bag and PC them. It's just like the original packaging.
you seal it before you pressure cook it?
This video is really useful. A very big contribution to the myco community. Thanks!
I love your videos! I just take your syringe apart, rinse off particulate matter then drop in alcohol for 1 hour, reassemble irrigate with distilled water and store for next use in mason jar. Been using this technique for 8 years and have had no problems. I guess more than one way to skin a cat.
So is filling it with distilled water until your ready to use it keep the plunger tube clean?
Is distilled water sterilized though?
@@droidnick No. However it's on a par with reverse osmosis water for ppm bacteria counts. To sterilize place in mason jar and pressure cook for 30 min at 15 psi. Have a port on the lid so you can keep sterile when you need to transfer to another container. You can use 4 jars and make about a gallon quickly.
Good to know , definitely will give it a try! Take care, peace ✌️ from Welland Ontario Canada 🇨🇦
Great idea! Thanks for sharing, I have never come across this.
Awesome, thanks 🙏
Good info! i was wondering if i could use my syringes again.
Awesome. Thanks for the comment Derek!
Awesome idea. Thanks!
man i love your videos. You're a great teacher
Thank you seansy! 😊
How do I get steril water? I want to Hydrate a dry mushroom for a transfer!
Im new to the game, but I found putting your used syringes in a mason jar with a lid modified or unmodified in the PC t for 30 minutes. Work perfect. Never had any warped or melted syringe, so far. Been in the game for 1 year.
Do I need to take it apart? Or just drop the needle and Syringe?
Depends what it's made out of. Even if the body stands up to the heat the black part won't. It's very succeptable to heat and solvents. The best option is to just use glass syringes and PC them.
Exactly what I needed to know! Thanks! Love your channel
Glad to hear that! Thanks for watching ☺
Alcohol + boiling water work well. Had no ill effects. Rinse them with alcohol and boiling water a few times. You can alternate from cold to hot a few times to force any bad mold spore cell walls to get destroyed.
No ill effects but not necessary for a syringe
Always grade A content. Thanks man
Does it have to be a particular type of water? Eg distilled? Drinking water? Or tap? Does it matter?
Nope
@@Mycophilia so any. It doesn’t matter. Thanks for the quick reply ! :)
Thanks for the vid. If its going to be reused and deformation during pressure sterilization is an issue, why not just use reusable glass syringes?
Using this method you won’t have to worry much about deformation, and don’t have to be careful about accidentally breaking it. Also much cheaper than a glass syringe.
very useful. cheers Sage
Glad it was helpful!
very unique content!! great youtuber!!
Thank you!
Great video.
Thanks!
What sizes or syringes and needles do you recommend having on hand?
Take syringe apart put it in boiling water for 5-10mins boom you have a stairalize syringe. Ive never had a syringe melt before maybe you can autoclave them? I feel like most syringes can take boiling water these days
Yeah I was wondering why he is tediously sucking up the boiling water, letting it sit, and pushing it out -- rather than just disassembling the syringe and leaving it inside the boiling water for some time. Seems much, much easier and simpler to do it this way than the method he shows in the video, and I'm all about maximizing efficiency 🤘🏻
I'm also wondering if I can just leave the disassembled syringe to soak in a solution of hypochlorous acid, which is the cleaning solution I use for everything instead of isopropyl alcohol. I'd imagine that would work just as well, and it would take about the same amount of time.
What size needle do you use for drawing your own LC into the syringe?
16 gauge
thank you
Your hands and that syringe had me anxious xD 😅
I got PTSD from accidentally pricking my finger 🙃
I actually accidentally harpooned my finger trying to get the cap off one of my needles lol 18 guage needle 💉 right through my glove , then the blood came , figured I had better start over lol 😂
@@whitefordpipeshandmadebymi7238 💀
love your videos bro❤
Thanks 😊
i was thninking about a grain covered in agar like hydroseeding with plants. have you tried any rooting hormones on mushrooms?
No
Danke..
Couldn’t you just put them in a jar of 70% iso for a couple days?
How about sucking Isopropyl Alcohol up into the syringe and then rinsing with Boiling Water?
Same here. This is the way. Endospores only germinate at certain temps so cold then hot a few times and alcohol are a great way to improve the sterility of the syringes.
Unnecessary as there are no endospores present in a syringe
Unless you happened to make grain water LC and your sterilization procedure was improper, and sucked that up into the syringe.
Nice tip. Could you also suck up Isopropyl alcohol and dip the parts that didnt get the bouling water into that?
Boiling water should have permeated everything, just take the syringe apart before boiling. ISO will dirty things as it sanitizes, not sterilize.
Thanks 😊😊😊😊
If boiling water is good enough for spore syringes, what is the purpose of autoclaving syringes? I've begun to load syringes with 10cc of water, wrap them in foil and sterilize them if there is extra space in the PC. It's quite convenient to have 10cc increments of sterilized water on hand. I've never made MSS syringes before -- do you think I could let some spores drop into a container, and then inject the presterilized water into the container and draw it back up to make a MSS?
There’s no need to autoclave syringes. You can also just pull up 10cc of boiling water as I show in this video after rinsing out your syringes with boiling water a few times (as I have shown in this vid).
As for making MSS, yes that is the way to do it, ofc in a SAB or flowhood etc… The best medium I found to mix the water and spores in is a shot glass. With plastic the spores tend to stick, and anything wider than a shot glass becomes a challenge to suck into the syringe.
5:05 it's where the video start's
No actually, it starts at 0:00 🙃
I found that the black plunger part splits apart when I PC them.
Leaving a comment just for algorithm!
Love the content Myco
Thanks Dylan!
Aren't those really cheap anyways?
thank you i took the syringe with the water since i don't want to expose the barrel
I was thinking you could just use alcohol, maybe a half hour soak time. That would have to sterilize it right?
Fine I'll subscribe ❤
Cheers 😂
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Mind reader!!
I’m assuming you would prefer to use distilled water for this?
I have hard water, which tends to leave crust at the bottom of my kettle and such…. So yes to distilled water?
I use tap water but if you have hard water, definitely distilled
A couple weeks ago, before I got my agar, I inoculated a popcorn grain jar with MSS, and I'm thinking I may not have left enough moisture in the kernels before pressure cooking, so I want to reuse a syringe to add more water through the injection port. I've used syringes for BRF in the past without issue, but I know popcorn is more susceptible to contaminants. Is it worth trying to inject more water after cleaning up a syringe? The main reason I'm thinking I didn't leave enough moisture in the kernels is because there was actually water at the bottom of the jars after I sterilized my last couple jars, which the popcorn quickly absorbed after shaking the jar 24 hours later. The first two had noticeable water on the sides of the glass but I didn't check for, or see water at the bottom. Lastly, do they dry up relatively quickly, is maintaining moisture and issue with gas exchange ports? Thank you in advance and I apologize for the long post!
I had some extremely dry grains when I was trying out the flash prep TEK. I ended up shooting 30cc of water each over 2 separate SAB sessions (the first one was not enough). Out of 10 jars or so about 4 contaminated when all was said and done. It’s worth it if it’s not colonizing, you don’t have much to lose at this point.
@Mycophilia great, thank you for the reply!. I took a closer look and saw the tiniest bit of condensation in the jar so decided to check back in another week and see how they're doing. One of them is doing quite well. The other two are pretty slow
What about the bleach solution? Does it work instead of using the pressure cooker?
if i use the syringe for LC, i just have to take extra sips of boiling water? thanks
Definitely wouldn’t hurt to do a few extra rinses
I like using chlorahexadine .... then alcohol......
Would it be overkill to wrap the syringe and needle in foil and PC them after doing this?.
Yes, in that case just PC it from the get-go.
@@Mycophilia Thanks man!, keep up the awesome and informative videos.
The "tenements" of Mycophilia! Okay... did you mean tenets?
😆 yes
🤣
Tenements lol
Why pressure cook? You can just soak the thing inside and out with isopropyl.
Nobody’s advocating PCing here. Please watch the video
Aren't those so cheap. I used to get them FREE from pharmacies.
Why not just suck up 100% isopropyl alcohol a few times instead of boiling water? Wouldn't that kill all bacteria?
Not as effective as heat. Same reason why you sterilize grains with heat, not iso.
Most people who have had babies would know sterilising baby bottles etc in boiling water is centuries old , not sure why so many people complicate sterilising things , not rocket science .
💯, people love to overcomplicate things 😂
@@Mycophilia …So true lol 👍
It because most of society is stupid as fuck now
Not if your baby is fed naturally. Not everyone feeds their babies fake milk out of bottles.
Did you ever tried to sterilize with rubbing alcohol?
no need, why do it when you can just boil it?
@@Mycophilia Thank you 🌹. I am looking for a quick solution when I do not have time to boil the syringes. So, I thought that it might do the job if they rinsed in bleach and then rinsed in 91% alcohol, and after that, the first sterilized liquid was discarded. Maybe it's a crazy idea🥴?
🍄🍄🍄
bro your content is to good to be sabotaged by the audio...
a 100 pack is like 20$ why would you bother doing this?
It takes a few mins and reduces waste. Why not?
@@Mycophilia I guess but personally, I would just use new ones. If you can't access them for some reason I understand the purpose of sterilizing and reusing.
@@linuxlinux9914 I have plenty. I just don’t like wasting things when they can easily be reused.
Why not just suck the boiling water into the syringe then leave the syringe in the boiling water for a few minutes.
Not necessary to put it inside the boiling water really, as long as the inside is sterilized.
myellowww
Blah
Waste of time and energy. I just paid $7 for 100 disposable 10ml Luer lock syringes with needles. With the energy cost and the time it takes to do this, I highly recommend against it. If you just have a syringe or two, also remove the rubber ftom the plunger and just soak it all in a jar of 70+% Etanol, which you can use for cleaning your workspace later and thereby not waste anything.
Ethanol does not sterilize like boiling water does, that is why ethanol is not used. It’s not that much time lol, just boil the syringe for a couple of minutes. Help the environment out a little.
it costs WAAAYYY more to sterilize these syringes than to buy a few brand new ones for a couple of dollars.... Sorry mate, but this "method" is not usefull at ALL !