The fact that you into so much detail is insane to me, I have so much respect for you, though I rarely need this much detail. Your videos are extremely useful for those who are self teaching
Anyone else watching this before tomorrow’s exam? I’ve never understood biochemistry so now I’m watching this in 2x speed because I don’t have much time.
The severe conditions of the protein hydrolysis really makes you wonder in awe at how the enzymes break the proteins in food down in mere hours in room temperature!!!
Hiii, I am a bit confused so if you could please clarify this I would be greatful (OFFICIAL A-LEVEL CHEM IN 3 DAYS!!) You said at 35:18 that only one enantiomer of the SUBSTRATE can fit into the active site of the enzyme. Wouldn't it be that only one enantiomer of the ENZYME can fit into the substrate? Because saying that the substrate has an enantiomer is assuming that the substrate is also chiral, and surely not all substrates are chiral? Please clarify this ASAP thank youu!!
nope, i dont think he made a mistake, search stereospecific enzymes and youll get a better understanding - goodluck for the exams on Monday, lets hope its easier then paper1! Edit: "Enzymes | A-level Chemistry | OCR, AQA, Edexcel" ua-cam.com/video/0I8aPGKxwAU/v-deo.html -> go to 2:42, he explains it
An enzyme is usually specific to the molecule it is breaking down (at least for A Level), so literally no other molecule will fit into that specific enzyme, hence the "lock and key" description. So in the case that an enzyme 'lock' has a specific substrate 'key' that is chiral and has enantiomers, that enzyme is only fitted to break down one of those enantiomers, hence is stereospecific. Just think of it like enzymes are really picky, and will only break down one specific molecule, and not even their enantiomer will do. Either way the enzymes discussed in proteins tend to be for chiral molecules, so they are stereospecific and only allow one enantiomer to be catalysed by it. I hope this helps, currently revising for Paper 2 and 3 as well this week, my bad if I got something wrong with what I said but I'm pretty sure that's what's happening here. Wishing you and me both the best of luck in the next 2 papers, we got this.
10:15 might be my lack of knowledge But I think that’s a typo at the bottom right? The Hydroxy will become deprotenated in high pH not the Amino group Otherwise you’d have Nitrogen with one Hydrogen bonded to it right?
Every three nucleotides codes for 1 amino acid. That would mean 2000 amino acids. However after transcription of DNA the mRNA has some sections of bases removed by enzymes. These removed nucleotides are called introns and they dont code for an amino acid
Timestamps:
AMINO ACIDS (3.13.1)
2:28 - Amino Acids Overview
4:39 - Naming Amino Acids
7:17 - Zwitterions + Amphoteric Properties
**
PROTEINS (3.13.2)
10:13 - Thin Layer Chromatography
19:38 - Proteins Overview
20:56 - Formation of dipeptides + polypeptides
22:58 - Hydrolysis of a polypeptide (overview)
24:15 - Primary, Secondary and Tertiary Structure
28:12 - Hydrogen + Disulphide bonds in proteins
**
ENZYMES (3.13.3)
31:40 - Enzymes overview
34:06 - Specificity of Active Site + Stereospecificity
37:25 - (Competitive) Inhibitors
39:10 - Inhibitor drugs
**
DNA (3.13.4)
42:33 - DNA Overview + Nucleotide Structure
44:32 - The 4 DNA Nucleotides (structure)
46:37 - Polynucleotide formation
49:14 - Double Helix Structure + Base Pairing/H bonding
**
ACTION OF ANTICANCER DRUGS (3.13.5)
55:32 - Cis-platin as an anticancer drug
thank u :)
legend
may allah shine his light on you the way you have on me
ameen
Ameen Sum Ameen
Ameen
Ameen
Ameen
The fact that you into so much detail is insane to me, I have so much respect for you, though I rarely need this much detail. Your videos are extremely useful for those who are self teaching
Thanks very much, I appreciate it!
Anyone else watching this before tomorrow’s exam? I’ve never understood biochemistry so now I’m watching this in 2x speed because I don’t have much time.
Yup except for me this is the only topic i understand fully because i do biology
The severe conditions of the protein hydrolysis really makes you wonder in awe at how the enzymes break the proteins in food down in mere hours in room temperature!!!
“I don’t have hair myself” 😁
51:00 I just use " Apples grow on Trees and Cars in Garages"
Sir this video is amazing! My teacher isnt that good, so I'm using these to learn the content, you explain things much better than my teacher
Happy to help!
Awesome! I find this topic tough, I dont know why its in Chemistry, but this video really helped me understand it.
Glad it helped!
Cheers mate so helpful during Clovid isolating
No problem 👍
Holy sweet mother of neptune I am unbelievably cooked tomorrow
SAME! I havent even memorised the organic route and NMR
i’m crying rn, so cooked
UCAS clearing looking really good rn
what are u doing now lol
4:12 carbon is actually the heaviest metal as carbon forms diamond and about 1 gram of diamond weighs about 15g
carbon is not a metal lol
i smell the tism. how can 1g weigh about 15grams. delete this comment u embarassment
🤣
Suuuuuper helpful + thorough, thank you!
An excellent video
Glad you enjoyed it
does the hydrolysis of a protein need to be refluxed for 24 hours (as it says on the powerpoint) or 48 hours (like you say)
Googled it and it’s 24 hours according to chemguide
24 hours
Hiii, I am a bit confused so if you could please clarify this I would be greatful (OFFICIAL A-LEVEL CHEM IN 3 DAYS!!) You said at 35:18 that only one enantiomer of the SUBSTRATE can fit into the active site of the enzyme. Wouldn't it be that only one enantiomer of the ENZYME can fit into the substrate? Because saying that the substrate has an enantiomer is assuming that the substrate is also chiral, and surely not all substrates are chiral? Please clarify this ASAP thank youu!!
nope, i dont think he made a mistake, search stereospecific enzymes and youll get a better understanding - goodluck for the exams on Monday, lets hope its easier then paper1!
Edit:
"Enzymes | A-level Chemistry | OCR, AQA, Edexcel"
ua-cam.com/video/0I8aPGKxwAU/v-deo.html -> go to 2:42, he explains it
An enzyme is usually specific to the molecule it is breaking down (at least for A Level), so literally no other molecule will fit into that specific enzyme, hence the "lock and key" description.
So in the case that an enzyme 'lock' has a specific substrate 'key' that is chiral and has enantiomers, that enzyme is only fitted to break down one of those enantiomers, hence is stereospecific.
Just think of it like enzymes are really picky, and will only break down one specific molecule, and not even their enantiomer will do. Either way the enzymes discussed in proteins tend to be for chiral molecules, so they are stereospecific and only allow one enantiomer to be catalysed by it.
I hope this helps, currently revising for Paper 2 and 3 as well this week, my bad if I got something wrong with what I said but I'm pretty sure that's what's happening here. Wishing you and me both the best of luck in the next 2 papers, we got this.
beyond cooked
Soooo cooked
Do you reflux the hydrolysis of proteins for 24 hrs or 48 hrs?
Googled it and it’s 24 hours according to chemguide
may depend on the exam board you have but for AQA the answer they expect is 24 hours
This is so helpful thank you Sir🙏
You are welcome
10:15 might be my lack of knowledge
But I think that’s a typo at the bottom right?
The Hydroxy will become deprotenated in high pH not the Amino group
Otherwise you’d have Nitrogen with one Hydrogen bonded to it right?
No, it's comparing to the zwitterion. The zwitterion has NH3+ and the one at high pH has NH2. The hydroxy is already deprotonated as a zwitterion
2024 gang?
here 😓
Mannn🥲🥲🥲
ANYONE FOR 2024 PAPER 2 ?
@51:00 NickEh30 reference?
Thank you! Very helpful :)
No worries!
Is this for AS too?
no, this is part of a level only
Thank you so much!
thank you!!!
You're welcome!
Thank you!
Why DNA containing 6000 nucleotides on transcription and translation to protein contain 1000 amino acids?
Every three nucleotides codes for 1 amino acid. That would mean 2000 amino acids. However after transcription of DNA the mRNA has some sections of bases removed by enzymes. These removed nucleotides are called introns and they dont code for an amino acid
I’m cooked
31:10 this guy violates himself in every video.
Seems like a habit!
A LEVEL BIOLOGY GANGGGGGGGGGGG ASSESMLE