Amazing video. My genetics class at Temple U. has failed to communicate the concepts of this 6 minute video in 3+ weeks of lecture AND in person lab, where as this video is an elegant, cogent recount of the concepts, and practices core to Benzer's momentous discoveries. I can not thank you enough for this video.
wtf this makes so much sense ive been trying to understand this for days now. One question, does the A and B genes that code for clear plaques, are they same gene or not? does this mean there are two genes for that locus? And will a locus contain only gene or one phenotype?
They are two genes, defined by independent sequences coding for different proteins. Both proteins are necessary for the wild-type phenotype. If protein A or B are defective, the phage behaves as an rII mutant. The two genes are part of the rII locus: they are so close that originally the rII locus was thought to encode a single protein.
@@Genetics101 thank you! Another question, how is this different from intragenic recombination, if the recombinant has the wild type for each gene, won't it have a wild type offspring? How can you tell if complementation occured or intragenic recombination?
The video discusses how you can detect the presence of two genes. Essentially, when you cross “A+, B- “ x “A-, B+”, you get complementation whenever the two phages coinfect a cell. At high MOI, this is very, very frequent. This would not happen if the two mutations are in the same gene. Therefore, it pretty clear when intragenic recombination takes place. Watch the video again if this is not clear.
Amazing video. My genetics class at Temple U. has failed to communicate the concepts of this 6 minute video in 3+ weeks of lecture AND in person lab, where as this video is an elegant, cogent recount of the concepts, and practices core to Benzer's momentous discoveries. I can not thank you enough for this video.
Complicated topic covered in minimum time in a beautiful way. Thank you sir
Just want to say THANK YOU kind sir for posting these videos.
Sincerely,
A stressed out college student who has shitty professors and TA's
Excellent explanation,,,with very good skills
Thank you so much for saving me in just 6 minutes. The illustration really helped with my understanding!!
Clearest explanation I've heard, thank you so much!
Clear language and good drawings. Thank you!
Amazingly clear explanation. Thanks a lot!
Very concise and very helpful! Thank you :)
Love this video, thank you sir!
thank you! nice illustration!
Perfect explanation thank you so much!
Please suggest a book for recombination in bacteriophage... Please...
Thank you !
Thank u so much sir😊
wtf this makes so much sense ive been trying to understand this for days now. One question, does the A and B genes that code for clear plaques, are they same gene or not? does this mean there are two genes for that locus? And will a locus contain only gene or one phenotype?
They are two genes, defined by independent sequences coding for different proteins. Both proteins are necessary for the wild-type phenotype. If protein A or B are defective, the phage behaves as an rII mutant. The two genes are part of the rII locus: they are so close that originally the rII locus was thought to encode a single protein.
@@Genetics101 thank you! Another question, how is this different from intragenic recombination, if the recombinant has the wild type for each gene, won't it have a wild type offspring? How can you tell if complementation occured or intragenic recombination?
The video discusses how you can detect the presence of two genes. Essentially, when you cross “A+, B- “ x “A-, B+”, you get complementation whenever the two phages coinfect a cell. At high MOI, this is very, very frequent. This would not happen if the two mutations are in the same gene. Therefore, it pretty clear when intragenic recombination takes place. Watch the video again if this is not clear.
Thank you sir
🥰🥰🥰❤❤❤
I found Naveen Patnaik's brother.. 😂
Thank you!