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Biochemist Melo
Приєднався 17 гру 2020
Hi my name is Melody (PhD) and welcome to my channel dedicated to science and biochemistry. My goal is to make science easy and I hope you find the content interesting and inspiring.
👩🔬 Biochemist Laboratory Work Vlog - Days in my life as a female scientist - ولاگ آزمایشگاه بیوشیمی
#biochemistlab #dayinlife #lab
#dayinthelife #biochemistry #vlog #labvlog #workdayvlog #iranianscientist
#dayinthelife #biochemistry #vlog #labvlog #workdayvlog #iranianscientist
Переглядів: 432
Відео
Peptide Array Synthesis on a Cellulose Membrane
Переглядів 371Рік тому
Peptide Array Synthesis on a Cellulose Membrane
Preparing Lipids for extrusion, from chloroform to aqueous buffer || Practical Biochemistry
Переглядів 8612 роки тому
example of preparing a lipid mixture of 80% dimirystoyl phosphatidyl serine (dmps) and 20% dimirystoyl phosphatidyl choline (dmpc) by mass #biochemistry #lipids #extrusion #liposome #vesicle #science #scientist #stem #tutorial #how to use lyophilizer #lyophilizer #freezedryer #how to use freeze dryer
🧪Trypsin Digestion - SDS-PAGE to compare protein protection/stability || Practical Biochemistry
Переглядів 5032 роки тому
#science #biology #laboratory #labtech #scienceasmr #tutorial #lab #biochemist #sdspage #protease #digestion
🔬Hitachi HT7800 Transmission Electron Microscope (TEM) step by step || Practical Biochemistry
Переглядів 1,7 тис.2 роки тому
#tem #microscopy #laboratory #science #biochemist #electronmicroscope #negativestain #hitachi #chemistry #visualization #fibril #liposome #میکروسکوپ #آزمایشگاه #بیوشیمی #phd #هیتاچی #tutorial #techniques #scienceasmr #asmrvideo
🔬How to Prepare and Carbon Coat Grids for Electron Microscopy TEM part 1 || Practical Biochemistry
Переглядів 3,8 тис.2 роки тому
part2: ua-cam.com/video/gyVXUYQ2ric/v-deo.html part3: ua-cam.com/video/krEtoADp8fQ/v-deo.html In TEM imaging the support layer has to be as thin as possible due to the fact that the thickness and density of its material influences image resolution and contrast. Another advantage of using carbon is that, its surface properties can be altered in processes like glow discharge, UV irradiation or ch...
🧪 How to do SAXS (Small Angle X-ray Scattering) Part 1 || Practical Biochemistry
Переглядів 4,1 тис.2 роки тому
this is part 1 of the saxs series. subscribe to learn about further processing, interpretation of data, and theories. #protein #biochemistry #saxs #xray #scattering #smallanglexrayscattering #laboratory #concentration #science #techniques #spectrometer #practical #scienceasmr #womeninscience #آزمایشگاه #اکس ری #بیوشیمی #اسمال انگل اکس ری اسکترینگ
💧How to Use a Nanodrop to Measure Protein Concentration || Practical Biochemistry
Переглядів 4,4 тис.2 роки тому
#protein #biochemistry #nanodrop #concentration #science #techniques #spectrometer #practical #scienceasmr #womeninscience
⚗Nickel Affinity Purification of His-tagged Proteins || Practical Biochemistry
Переглядів 4,7 тис.2 роки тому
⚗Nickel Affinity Purification of His-tagged Proteins || Practical Biochemistry
🧪Thermal Denaturation of Proteins using Circular Dichroism || Practical Biochemistry
Переглядів 2 тис.2 роки тому
🧪Thermal Denaturation of Proteins using Circular Dichroism || Practical Biochemistry
🧫Thioflavin T Fluorescence Assay for Detection of Amyloid Fibrils || Practical Biochemistry
Переглядів 2,2 тис.2 роки тому
🧫Thioflavin T Fluorescence Assay for Detection of Amyloid Fibrils || Practical Biochemistry
💧How To Use pHmeter to measure the pH of Solutions || Practical Biochemistry
Переглядів 2103 роки тому
💧How To Use pHmeter to measure the pH of Solutions || Practical Biochemistry
How to use the homogenizer to lyse cells (avestin) || Practical Biochemistry || آزمایشگاه بیوشیمی
Переглядів 9323 роки тому
How to use the homogenizer to lyse cells (avestin) || Practical Biochemistry || آزمایشگاه بیوشیمی
🔬How to use the Transmission Electron Microscope TEM 3 || Practical Biochemistry //میکروسکوپ الکترون
Переглядів 9823 роки тому
🔬How to use the Transmission Electron Microscope TEM 3 || Practical Biochemistry //میکروسکوپ الکترون
🔬How to do Negative Staining with Uranyl Acetate for Electron Microscopy 2 || Practical Biochemistry
Переглядів 2,4 тис.3 роки тому
🔬How to do Negative Staining with Uranyl Acetate for Electron Microscopy 2 || Practical Biochemistry
🧈 How to do Lipid Extrusion (Nanosizing Liposomes from LMV to SUV) || Practical Biochemistry
Переглядів 6 тис.3 роки тому
🧈 How to do Lipid Extrusion (Nanosizing Liposomes from LMV to SUV) || Practical Biochemistry
⚗ How to use Dialysis tubes to change your protein's buffer || Practical Biochemistry
Переглядів 8 тис.3 роки тому
⚗ How to use Dialysis tubes to change your protein's buffer || Practical Biochemistry
🧫 How to express protein using bacteria (E. coli) || practical biochemistry || آزمایشگاه بیوشیمی
Переглядів 4123 роки тому
🧫 How to express protein using bacteria (E. coli) || practical biochemistry || آزمایشگاه بیوشیمی
🧪 How to make 15% SDS PAGE gels *easy peasy* perfect for low mw proteins || practical biochemistry
Переглядів 2,3 тис.3 роки тому
🧪 How to make 15% SDS PAGE gels *easy peasy* perfect for low mw proteins || practical biochemistry
👩⚕️How to use a micro pipette || practical biochemistry
Переглядів 9113 роки тому
👩⚕️How to use a micro pipette || practical biochemistry
❄Vlog: A cold snowy day at the laboratory
Переглядів 903 роки тому
❄Vlog: A cold snowy day at the laboratory
how to do circular dichroism || practical biochemistry
Переглядів 10 тис.3 роки тому
how to do circular dichroism || practical biochemistry
vlog1: biochemistry laboratory vlog ولاگ آزمایشگاه بیوشیمی
Переглядів 8643 роки тому
vlog1: biochemistry laboratory vlog ولاگ آزمایشگاه بیوشیمی
Protein gets precipitated do you have any idea what should i do???
Can this assay be performed using an ELISA machine?
What kind of water u used in first step for hydrate the tube?
@KarisaAzharFauzi it was using my buffer 20mM tris in miliQ water
@@KarisaAzharFauzi whichever buffer you are dialyzing in can be used
Hi! Great videos! Could you write the reference number of your couvette? I use a foldable couvette 0,1mm but I have problems with evaporation and bubbles, especially when the probe is heated. Also do you have any tips for different results of the same sample? Sometimes I put on the same protein (from the same eppendorf), the same amount and I think that the results are different. Maybe its because the couvette that enhances evaporation? Thanks :D
great and informative video. Have you done thermal shift assay using qPCR it would be nice if you share a tutorial for TSA to find out ligand affinity for protein.
👏🏼👏🏼👏🏼
Are you in Toronto?
🙏🙏🙏🤚
☺️☺️💐
What a cool video!!! Really I like it 😀 my favourite YT channel is bio chemistry
Aw thank you so much for your support ☺️💐
Do you store lipid stocks by aliquoting them to prevent melting the entire bottle before weighing? Does this method help maintain the integrity of the lipids? Alternatively, can they simply be stored at -20°C?
How do you store your lipids? Do you aliquot them to prevent melting the entire bottle before weighing it?
سلام بانو موفق باشی البته من مهندس سازه هستم و چیزی از حرفه شما متوجه نمیشم و به قصد حمایت از شما لایک کردم سپاس از شما بانوی ایرانی
خیلی ممنون برای حمایتتون 😊🤗🤗
Hi Biochemist Melo. Can I please have the parameters that you used
Cool. Thnx
Isn't it necessary to give continuous stirring during the dialysis?
There is a little magnetic bar in the 4L container and it sits on a stirrer.
I wish you do some ASMR vídeos 😊
I hear a lovely iranian accent ❤
Yes I'm Iranian 🇮🇷
@@biochemistmelo Wishing you success in your job! 🤞 🍀 I am also a TEM engineer with a background in Materials Science, but now I am a cryo-EM laboratory director working with biologists ☺️
@farzaadkhaan wow that is amazing. Microscopy became my favorite technique in my PhD, so for my post-doc I hope to find a lab where I can learn cryo-EM.
Amei encontrar seu canal ❤
Thank you so much 💓
How to plot this data in graph pad ?
you and your voice are both so sexy
you fine af
Thank you
the solution is NOT simply amyl acetate! it is nitrocellolose (parlodion) DISSOLVED IN amyl acetate. 0:44 😊
Thank you
Very dirty carbon coater
THANK YOU!
🙏🙏
Hi, Could you tell me how did you calculate these volumes? Thank you!
Hi depends on the % of gel you want to make
Madam, are you Arab??
I would highly advise you wearing gloves at all times and following a more detailed cleaning procedure. From my experience this will ruin you quite some work you are doing here.
I've been doing this for 8 years and always check my liposomes with TEM. I wear gloves when I'm working because if I didn't I would see it on the TEM. You should worry about yourself.
awesome bands, congrats!
what is peptide array by SPOT? and can we use this methods for understand pro-pro interaction as well?
Yes you can have a peptide from one protein and add the other protein, followed by antibodies and detect interactions
Hi Melody, this video is phenomenal and is helping me with my PhD and Mashallah thank you 🙏
You are very welcome 🙏
Hello, can you share your email with me?
Can I ask why
There are several issues with operating the TEM like that. Please be careful. I would carefully follow this other video instead: ua-cam.com/video/noE_F1o1XmA/v-deo.html
Can you please share the details (product details and catalogue number) for the extruder you used?
Omg you should make an asmr channel. Very relaxing and informative!
Thank you 😊
Hello I'm a high school senior and I was wondering if possible to set up a biochemist informational interview regarding your career and further insight into it?
Hello. Yes definitely 😊
@@biochemistmelo How would I contact you directly to set the meeting?
I understand you'll probably be unable to do that I'll put the questions down. -Name of person, -Company they work for, -Years of experience, -Contact, -if available, business card. Questions: 1. How did you end up in this career? 2.How do you handle stress/mental health? 3. What qualifications are needed for your job?Do you have any pointers or suggestions for me when pursuing this career? 4. Do you have any regrets about this career choice?Why or why not? What are some negative things about this career choice?
Can you tell what you add from the brown bottle after the destain.
Another stain
Can you do a video on ANS fluorescence spectroscopy
Melody, great job on this video.
Thank you
hi dear, jut verifying, what grids are you using? and I assume there is no previous carbon support on these grids, you are making the support correct?
Hi dear. I have to check the detail but there is no carbon support on them initially and this is what the video shows.
nice
nice
nice
do we have to weigh the dialysis bag before putting it in the buffer
No dear. You just use your eyeballs to estimate how much you need.
Can you do SAXS part 2 video
Yes. Thank you for reminding me :).
Can you use this one protein samples which contains lots of protein? Or only for a single purified protein? Like for example I’m using a serum sample after depleting albumin and igGs. After performing Western blot I found that the bands were much larger than target protein size. I also tested it with different antibodies but the results were always much larger (almost double). So I thought I may need to take another look at concentration measurements cause perhaps the sample is too concentrated so here I am.
What are you trying to determine?
@@biochemistmelo I want to determine the difference of a specific protein level between patients and healthy controls in serum samples using western blot.
Can you explain how to analyse data in Gift software
Hi, the best software I recommend is ATSAS from Embl-hamburg
@@biochemistmelo can you make one video of ATSAS software
How much is this hand extruder in dollar please, and where can i get it online with cheapest price? Also is there a plastic one that is cheaper?
I think around $1000 but they are standard and have a mix of metal and plastics in them. Ours is from Avanti Polar Lipids (extruder kit)
Hi, thank you for this excelent video! I have some questions (probably silly ones). Situation: a experiment where i used a buffer as blank and the software was set to execute baseline correction. Does it means the further spectra obtained from samples will be already subtracted from buffer? Do i still need to click on baseline to manually adjust it after smoothing? If so, how i do the adjusting?
Very nice❤ i am wondering why not directly extruder after lipid rehyration with water. Can I omit the lyophilization step?
That is part of the rehydration step. I don't recommend skipping it. You can see a clear difference in how the lipid looks. In the first lyophilization step it looks very dense but after the second, it will look very fluffy and you know that it's been rehydrated.
@@biochemistmelo thanks!i have used this method to prepare my liposome,it's very useful😊