Відео

Protein gets precipitated do you have any idea what should i do???

Can this assay be performed using an ELISA machine?

What kind of water u used in first step for hydrate the tube?

Hi! Great videos! Could you write the reference number of your couvette? I use a foldable couvette 0,1mm but I have problems with evaporation and bubbles, especially when the probe is heated. Also do you have any tips for different results of the same sample? Sometimes I put on the same protein (from the same eppendorf), the same amount and I think that the results are different. Maybe its because the couvette that enhances evaporation? Thanks :D

great and informative video. Have you done thermal shift assay using qPCR it would be nice if you share a tutorial for TSA to find out ligand affinity for protein.

👏🏼👏🏼👏🏼

Are you in Toronto?

🙏🙏🙏🤚

What a cool video!!! Really I like it 😀 my favourite YT channel is bio chemistry

Do you store lipid stocks by aliquoting them to prevent melting the entire bottle before weighing? Does this method help maintain the integrity of the lipids? Alternatively, can they simply be stored at -20°C?

How do you store your lipids? Do you aliquot them to prevent melting the entire bottle before weighing it?

سلام بانو موفق باشی البته من مهندس سازه هستم و چیزی از حرفه شما متوجه نمیشم و به قصد حمایت از شما لایک کردم سپاس از شما بانوی ایرانی

Hi Biochemist Melo. Can I please have the parameters that you used

Cool. Thnx

Isn't it necessary to give continuous stirring during the dialysis?

I wish you do some ASMR vídeos 😊

I hear a lovely iranian accent ❤

Amei encontrar seu canal ❤

How to plot this data in graph pad ?

you and your voice are both so sexy

you fine af

the solution is NOT simply amyl acetate! it is nitrocellolose (parlodion) DISSOLVED IN amyl acetate. 0:44 😊

Very dirty carbon coater

THANK YOU!

Hi, Could you tell me how did you calculate these volumes? Thank you!

Madam, are you Arab??

I would highly advise you wearing gloves at all times and following a more detailed cleaning procedure. From my experience this will ruin you quite some work you are doing here.

awesome bands, congrats!

what is peptide array by SPOT? and can we use this methods for understand pro-pro interaction as well?

Hi Melody, this video is phenomenal and is helping me with my PhD and Mashallah thank you 🙏

Hello, can you share your email with me?

There are several issues with operating the TEM like that. Please be careful. I would carefully follow this other video instead: ua-cam.com/video/noE_F1o1XmA/v-deo.html

Can you please share the details (product details and catalogue number) for the extruder you used?

Omg you should make an asmr channel. Very relaxing and informative!

Hello I'm a high school senior and I was wondering if possible to set up a biochemist informational interview regarding your career and further insight into it?

Can you tell what you add from the brown bottle after the destain.

Can you do a video on ANS fluorescence spectroscopy

Melody, great job on this video.

hi dear, jut verifying, what grids are you using? and I assume there is no previous carbon support on these grids, you are making the support correct?

nice

nice

nice

do we have to weigh the dialysis bag before putting it in the buffer

Can you do SAXS part 2 video

Can you use this one protein samples which contains lots of protein? Or only for a single purified protein? Like for example I’m using a serum sample after depleting albumin and igGs. After performing Western blot I found that the bands were much larger than target protein size. I also tested it with different antibodies but the results were always much larger (almost double). So I thought I may need to take another look at concentration measurements cause perhaps the sample is too concentrated so here I am.

Can you explain how to analyse data in Gift software

How much is this hand extruder in dollar please, and where can i get it online with cheapest price? Also is there a plastic one that is cheaper?

Hi, thank you for this excelent video! I have some questions (probably silly ones). Situation: a experiment where i used a buffer as blank and the software was set to execute baseline correction. Does it means the further spectra obtained from samples will be already subtracted from buffer? Do i still need to click on baseline to manually adjust it after smoothing? If so, how i do the adjusting?

Very nice❤ i am wondering why not directly extruder after lipid rehyration with water. Can I omit the lyophilization step?