Synthetic Seed MICRO PROPAGATION
Meaning and concept of in vitro culture and micro-propagation
Historical milestones of in vitro culture and micro-propagation
Advancement and future prospects of micro-propagation
Totipotency, dedifferentiation
Tissue culture methodology: Sterilization techniques
Synthetic and natural media components
Growth regulators used in tissue culture media
Environmental requirement
Genetic control of regeneration
Plant regeneration pathways - Organogenesis and Somatic embryogenesis
Micro-propagation- Definition, methods, stages of micro-propagation and its significance
Axillary bud proliferation approach- Shoot tip and meristem culture
Organogenesis- Purpose, methods and requirements for organogenesis, indirect and direct organogenesis
Somatic embryogenesis- Procedures and requirements for organogenesis, indirect and direct embryogenesis
Differences between somatic and gametic embryogenesis
Synthetic seed-Concepts, necessity, procedure and requirements for production of synthetic seeds
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