This presentation by Dr Linzy Elton from the Centre for Clinical Microbiology, UCL, UK, gives an overview of the recently published Oxford Nanopore Technologies tuberculosis drug resistance and lineage diagnostics pipeline. This video covers each of the steps needed to optimise sequencing of M. tuberculosis:
- Culture methods
- DNA extraction
- DNA quantification (and the importance of good DNA)
- Library preparation kits
- Basecalling algorithms
- Analysis programmes
- Logistical considerations for LMICs
For more information about the tuberculosis sequencing pipeline, including more detailed tutorials, links to the paper and laboratory manual, please visit PANDORA-ID-NET's TGHN hub page: pandora.tghn.org/sequencing/tuberculosis-sequencing-pipeline/
For more general Oxford Nanopore sequencing tutorials, please follow this link: pandora.tghn.org/sequencing/sequencing-tutorials/
This video was recorded as part of training provided for the the National Institute for Medical Research Tanzania (NIMR), permission for recording was obtained from participants. For image credits and links from the slides, or for further questions on the pipeline, please email pandora.id.net@gmail.com