Annelise Snyder
Annelise Snyder
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Lecture 8a: Comprehensive Immune Response to Infection
UCSD Extension School: Applied Immunology (BIOL-40371)
Summer Quarter 2021
This lecture summarizes the coordination of a comprehensive immune response to a hypothetical bacterial infection in the skin, focusing on the integration of innate and adaptive cell types needed to accomplish the generalized goals of a successful immune response. This overview includes a focus on chemokine gradients and the essential role that they play in the spatial organization of immunity, as well as the immunosuppressive cells and pathways that resolve inflammation to restore tissue homeostasis following the clearance of infection.
All figures are either from Janeway's Immunobiology (9th ed.) where noted, or my own original figures.
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Відео

Lecture 10c: Cancer Immunotherapy
Переглядів 5 тис.3 роки тому
UCSD Extension School: Applied Immunology (BIOL-40371) Summer Quarter 2021 This lecture summarizes the mechanism of action for several forms of cancer immunotherapy, including cancer vaccines, monoclonal antibodies, and chimeric antigen receptor (CAR) T cell engineering. All figures are either from Janeway's Immunobiology (9th ed.) or other publications where noted, or my own original figures.
Lecture 10b: Cancer Biology + Immune Evasion
Переглядів 5 тис.3 роки тому
UCSD Extension School: Applied Immunology (BIOL-40371) Summer Quarter 2021 This lecture presents an overview of the process of oncogenic transformation, immune surveillance of transformed cells, and the numerous mechanisms that tumor cells use to evade or subvert immune recognition and targeting. All figures are either from Janeway's Immunobiology (9th ed.) or other publications where noted, or...
Lecture 10a: Mucosal Immunology
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UCSD Extension School: Applied Immunology (BIOL-40371) Summer Quarter 2021 This lecture summarizes specialized immune responses that are localized at mucosal surfaces, and how they work to both promote rapid responses to infectious pathogens while maintaining mucosal tolerance to commensal microbes and food antigens. All figures are either from Janeway's Immunobiology (9th ed.) where noted, or ...
Lecture 8c: SARS-CoV-2 Vaccines
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UCSD Extension School: Applied Immunology (BIOL-40371) Summer Quarter 2021 This lecture summarizes the design and regulation of current SARS-CoV-2 vaccine products, both in current development and those that are approved for use in patients. All figures are either from Janeway's Immunobiology (9th ed.) or other publications where noted, or my own original figures.
Lecture 8b: Vaccine Design
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UCSD Extension School: Applied Immunology (BIOL-40371) Summer Quarter 2021 This lecture summarizes vaccine design, different antigenic components used in various vaccine formulations, their safety and mechanism of action, and their importance in preventing infectious disease outbreaks. All figures are either from Janeway's Immunobiology (9th ed.) or other publications where noted, or my own ori...
Lecture 7b: Flow Cytometry
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UCSD Extension School: Applied Immunology (BIOL-40371) Summer Quarter 2021 This lecture provides an introduction to flow cytometry techniques used for immunophenotyping both basic research and clinical patient samples. This lecture includes a discussion of sample flow plots used to diagnose various clinical pathologies, including B cell acute lymphoblastic leukemia (B cell ALL), severe combined...
Lecture 6c: Mouse Models
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UCSD Extension School: Applied Immunology (BIOL-40371) Summer Quarter 2021 This lecture discusses one of the most important model organisms used for in vivo immunology research: inbred mice. It provides an overview of the differences between transgenic and knockout mice and discusses examples of different disease models and tissue-specific expression systems that are commonly used by immunology...
Lecture 5c: B Cell + Antibody Functions
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UCSD Extension School: Applied Immunology (BIOL-40371) Summer Quarter 2021 This lecture summarizes the different forms of antibody classes (isotypes), and the primary functions of antibodies in mediating humoral immune responses. All figures are either from Janeway's Immunobiology (9th ed.) where noted, or my own original figures.
Lecture 4a: Lymphocyte Antigen Receptors
Переглядів 9 тис.3 роки тому
UCSD Extension School: Applied Immunology (BIOL-40371) Spring Quarter 2021 This lecture presents an overview of the structure of lymphocyte antigen receptors (immunoglobulin and the TCR), and discusses the genetic processes of V/D/J recombination used to generate diverse repertoires of these receptors. All figures are either from Janeway's Immunobiology (9th ed.) where noted, or my own original...
Lecture 9c: Transplantation Immunology
Переглядів 6 тис.3 роки тому
UCSD Extension School: Applied Immunology (BIOL-40371) Spring Quarter 2021 This lecture summarizes the mechanisms of immune responses mounted against organ transplants (allografts). It covers major and minor histocompatibility antigens, direct and indirect allorecognition, hyperacute graft rejection mediated by alloantibodies, and the mechanism of action of several immunosuppressive drugs admin...
Lecture 9b: Autoimmunity
Переглядів 5 тис.3 роки тому
UCSD Extension School: Applied Immunology (BIOL-40371) Spring Quarter 2021 This lecture covers autoimmune diseases, a group of pathologies where inflammatory immune responses are inappropriately used against self antigens, leading to immune attack of otherwise healthy tissue. It discusses the mechanisms of central and peripheral tolerance and how they may be broken by both genetic and environme...
Lecture 9a: Allergy
Переглядів 2,6 тис.3 роки тому
UCSD Extension School: Applied Immunology (BIOL-40371) Spring Quarter 2021 This lecture summarizes how the immune system is inappropriately activated during allergic responses to innocuous environmental antigens, called allergens. It summarizes the 4 types of allergy (or hypersensitivity) responses, and ends with a discussion of the hygiene hypothesis and its attempt to explain why incidence of...
Lecture 7a: Clinical Diagnostics
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UCSD Extension School: Applied Immunology (BIOL-40371) Spring Quarter 2021 This lecture provides an overview of several commonly used clinical diagnostic tests used to characterize patient samples, each of which are based on principles of antibody-antigen recognition. This includes ABO blood typing, Coombs testing, ELISAs testing for fixed antigens, and leukocyte isolation for further functiona...
Lecture 6b: Antibody Techniques
Переглядів 2,2 тис.3 роки тому
UCSD Extension School: Applied Immunology (BIOL-40371) Spring Quarter 2021 This lecture discusses methods used to generate monoclonal and polyclonal antibodies. It also provides a brief outline of commonly used applications of antibodies in immunology research methods, including immunoprecipitation, sandwich ELISA, Western blot, immunofluorescence (IF) microscopy, flow cytometry, receptor neutr...
Lecture 6a: In Vitro Cell Types
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Lecture 6a: In Vitro Cell Types
Lecture 1b: Introduction to Immunology
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Lecture 1b: Introduction to Immunology
Lecture 5b: B Cell Signaling + Activation
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Lecture 5b: B Cell Signaling Activation
Lecture 4d: T Cell Function
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Lecture 4d: T Cell Function
Lecture 5a: B Cell Development
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Lecture 5a: B Cell Development
Lecture 4c: T Cell Signaling + Activation
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Lecture 4c: T Cell Signaling Activation
Lecture 4b: T Cell Development
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Lecture 4b: T Cell Development
Lecture 1c: Categories of Immune Responses
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Lecture 1c: Categories of Immune Responses
Lecture 1d: Lymphoid Organs and Lymphatics
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Lecture 1d: Lymphoid Organs and Lymphatics
Lecture 2c: Programmed Cell Death and The Complement System
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Lecture 2c: Programmed Cell Death and The Complement System
Lecture 2b: Innate Immune Receptors and Signaling
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Lecture 2b: Innate Immune Receptors and Signaling
Lecture 2a: Introduction to Innate Immunity
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Lecture 2a: Introduction to Innate Immunity
Lecture 3c: Innate Lymphoid Cells
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Lecture 3c: Innate Lymphoid Cells
Lecture 3b: Antigen Presentation
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Lecture 3b: Antigen Presentation
Lecture 3a: Antigen Processing
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Lecture 3a: Antigen Processing

КОМЕНТАРІ

  • @lvn2410
    @lvn2410 2 дні тому

    Best video ever

  • @solanohernandezjoseantonio3782
    @solanohernandezjoseantonio3782 10 днів тому

    Does anyone have any idea about how to calulate the Mean Fluorescence Intensity? I am using a Sysmex Ploidy Analyser.

  • @junaidrehmani9829
    @junaidrehmani9829 Місяць тому

    Goldmine

  • @charlescobbs2561
    @charlescobbs2561 2 місяці тому

    Phenomenal lecture

  • @AntonShostak
    @AntonShostak 3 місяці тому

    Thanks for sharing

  • @safimoshkani8495
    @safimoshkani8495 5 місяців тому

    Thank you, very clear explanation

  • @abbaskassir4912
    @abbaskassir4912 6 місяців тому

    Thanks ❤

  • @zhang19242Liv
    @zhang19242Liv 6 місяців тому

    where is lecture 1a?

  • @_M3gh
    @_M3gh 7 місяців тому

    Thank you!

  • @songthanh896
    @songthanh896 7 місяців тому

    Thank you very much 😊

  • @jayalalkj1576
    @jayalalkj1576 8 місяців тому

    thanks Annelise

  • @MaxfuzaKarimova-980
    @MaxfuzaKarimova-980 8 місяців тому

    I couldn't find an answer why even with perfect matched MHC there's chronic rejection of graft. Now I understand that besides major HCs there are also minor ones. Thank you so much

  • @mofeihuang9491
    @mofeihuang9491 8 місяців тому

    Nice lecture!

  • @shellodee
    @shellodee 8 місяців тому

    I have been looking for a video explanation of the T Cell activation processes as clear as this for the last 7 years. My son was born with ORAI1 deficiency and passed away in 2017. I knew what went wrong and could explain to someone who had no idea, but i wanted to know exactly where in the activation process it went wrong. Thank you. Amazing that through all the genetic coding that goes in to one human being, all it takes is the substitution of 1 letter twice in one gene to catastrophically collapse it all. . .

  • @gerardhoeltzel4690
    @gerardhoeltzel4690 9 місяців тому

    awesome lecture - phd student trying to better understand Tfh mechanisms. thanks so much!!

  • @goonther-l4q
    @goonther-l4q 9 місяців тому

    they dont require help. that's the whole point of being Tindependant antigens

  • @zhiyaoxie6511
    @zhiyaoxie6511 9 місяців тому

    thank you!

  • @muik99
    @muik99 10 місяців тому

    @Annelise, your presentation is clear, concise and very well presented. Thank you!

  • @june-fg7th
    @june-fg7th 10 місяців тому

    Thank you for the lecture. Helped me a lot to cleary understand the mouse model.

  • @mrunmayidalal260
    @mrunmayidalal260 11 місяців тому

    very clear, concise and helpful. thank you Prof. Snyder!

  • @rugogamujesse
    @rugogamujesse Рік тому

    I am writing to express my deepest gratitude for the immunology videos you have created and shared with the world. As a learner, I have found them to be incredibly fundamental to my understanding and appreciation of immunology. Your ability to simplify complex concepts and engagingly present them has made a significant difference in my learning journey. The depth and breadth of your knowledge, as well as your passion for the subject, shine through in each video. Your work has not only enriched my knowledge but also sparked a deeper interest in immunology. It is individuals like you who make learning accessible and enjoyable for many of us, and for that, I am truly grateful. Please continue to share your expertise and passion with the world. Your contributions are making a difference in ways you may not even realize. Thank you once again for your invaluable work.

  • @hussenmohammed350
    @hussenmohammed350 Рік тому

    doctor Snyder, I'm so grateful to you for this comprehensive course , but I wish to learn about immune response and cascade reactions of it at both cell & humoral mediated immunity in more details .

  • @thefriulander3595
    @thefriulander3595 Рік тому

    Hi Annelise... wonderful video.. I finally understand, I think... but I have a question. If you have a mouse model with a floxed gene and a Cre under the K18 promoter... what is the proper control mouse to use? Is it a wildtype mouse? Or a heterozygote mouse that has the Cre under the K18 promoter and an empty LxP cassette? Not sure... please help..

  • @spartanrabbit
    @spartanrabbit Рік тому

    thank you from Chile!

  • @il3mendo
    @il3mendo Рік тому

    TCR:which ones are the difference between: alpha, beta, gamma and delta ?

    • @mhutflash5512
      @mhutflash5512 8 місяців тому

      DOI: 10.1002/JLB.2MR1219-233R

    • @azca.
      @azca. 3 місяці тому

      The TCR subunits-alpha, beta, gamma, and delta-pair to form two distinct types of TCRs: alpha-beta (αβ) and gamma-delta (γδ). The alpha (α) and beta (β) chains pair to form the αβ TCR, which is the most common type and primarily recognizes peptide antigens presented by MHC molecules. The gamma (γ) and delta (δ) chains pair to form the γδ TCR, which recognizes a broader range of antigens, including non-peptide antigens, and is less dependent on MHC. αβ T cells are mainly involved in adaptive immune responses, while γδ T cells often play a role in innate immunity and tissue surveillance.

    • @il3mendo
      @il3mendo 3 місяці тому

      @@azca. That means that gamma delta works more with the innate immune system, Natural killer cells and eosinophilis? Does gamma delta t cell works in our mucosal barrier?

  • @il3mendo
    @il3mendo Рік тому

    Doc Cd3s+ with cd8+ and anti Tcr gamma delta + what does mean ? Same as Cd3s, cd4-,cd8- and anti Tcr gamma delta -?

    • @azca.
      @azca. 3 місяці тому

      "CD3s+ with CD8+ and anti-TCR gamma-delta+" indicates a T cell that expresses the CD3 complex, which is present on all T cells, along with the CD8 co-receptor, typically found on cytotoxic T cells. The "anti-TCR gamma-delta+" part means this T cell expresses the gamma-delta (γδ) T-cell receptor, which is less common and is involved in recognizing a broader range of antigens. "CD3s+, CD4-, CD8-, and anti-TCR gamma-delta-" describes a T cell that also expresses the CD3 complex but lacks both CD4 and CD8 co-receptors, making it a double-negative T cell. The "anti-TCR gamma-delta-" part means this T cell does not have the gamma-delta TCR, indicating it likely expresses the more common alpha-beta (αβ) TCR instead.

    • @il3mendo
      @il3mendo 3 місяці тому

      @@azca. Brother I love you for your answer. I have celiac disease and I was diagnosed as a non responsive celiac disease 2 years ago and I was pretty scared. I had a 50% of TCR gamma delta + and gamma delta - based on my 4th histological finding. Are the gamma delta double negative the expression of the lenght of the disease ? I was not able to produce any IgA antibodies apparently, and unfortunately few celiacs are going through this struggles. Since gamma delta t cell are not well studied yet, what change the TCR "polarity" ?

  • @omerabyelhaj4508
    @omerabyelhaj4508 Рік тому

    Really impressive! Many thanks for sharing

  • @FreakySteph
    @FreakySteph Рік тому

    why is there only one CDR primarly involved in antigen binding for TCRs whereas the CDRs in Antibodies are all equally equivalent in regards to antigen binding?

    • @azca.
      @azca. 3 місяці тому

      In TCRs, CDR 3 is primarily involved in antigen binding because it directly interacts with the peptide presented by the MHC molecule, which is the main focus of TCR recognition. CDR1 and CDR2 regions mainly contact the MHC itself, rather than the peptide. In contrast, antibodies bind directly to free antigens, allowing all six CDRs (three from each of the heavy and light chains) to equally contribute to diverse antigen interactions.

  • @paoe95
    @paoe95 Рік тому

    Amazing video!! So comprehensive, very detailed and has the perfect depth in this topic. Thank you so much for sharing this.

  • @ShadrackKipchirchir-m2p
    @ShadrackKipchirchir-m2p Рік тому

    i appreciate you've made immunology simpler

  • @meenakshikar
    @meenakshikar Рік тому

    this is so relevant to immunology researchers. a must watch for all graduate, PhDs and even postdocs!

  • @tnbn90
    @tnbn90 Рік тому

    Hi! Thank you for your videos. Just wanted to point out that kinases attach phosphate groups from ATP as opposed from free phosphate. Phosphorylases are the one that utilize free phosphate.

  • @arioche
    @arioche Рік тому

    thank you, you explain clearly

  • @adrianfiliberti
    @adrianfiliberti Рік тому

    Thank you Dear Annelise, super clear!, thanks again!

  • @surat8591
    @surat8591 Рік тому

    Amazing 👏 🙀

  • @codylewis2233
    @codylewis2233 Рік тому

    Thank you so much!

  • @victorprokop9343
    @victorprokop9343 Рік тому

    is it possible that excessive antigen exposure lead to the allergy epidemic? We think of our environment as "clean" but its not. Maybe the modern home is full of certain antigens like bed bug antigens, mold, and some microbiota that differs from a more "natural" habitat Could it be a matter of evolutionary imcompatibility?

  • @AdityaSingh-hh2jr
    @AdityaSingh-hh2jr Рік тому

    wow, very good

  • @hsianloon
    @hsianloon Рік тому

    best lecture series on youtube, great for non immunologist getting into it. Thank you

  • @cesarfiladelfo
    @cesarfiladelfo Рік тому

    What an excellent lecture! Thank you very much!

  • @vamseekumar9968
    @vamseekumar9968 Рік тому

    Thank you. But please Correct the history. It was used in India before it spread to China

  • @Stevenkigozi
    @Stevenkigozi Рік тому

    Great

  • @mahya8450
    @mahya8450 Рік тому

    Your lectures are helping me a lot with my exams. Thanks a lot for sharing 🙏❤

  • @Adam-vg7pu
    @Adam-vg7pu 2 роки тому

    Great lecture! I feel better prepared now for tomorrow's in-course assessment!

  • @songthanh896
    @songthanh896 2 роки тому

    Thank you so much!

  • @wogus322199
    @wogus322199 2 роки тому

    Hi thank you for the lecture! It helped a lot 😊

  • @ZIJUNXU
    @ZIJUNXU 2 роки тому

    Sorry, is the last slide correct? Ti antigens require T cell help and induce GC B-cell differentiation?

  • @MrMyfra
    @MrMyfra 2 роки тому

    well explained , well done, thanks

  • @kiomi8659
    @kiomi8659 2 роки тому

    Thank you for your clear explanation! This is great :)

  • @ChowhallmsqdcUSMC
    @ChowhallmsqdcUSMC 2 роки тому

    I appreciate for your lectures Annelise Snyder. Would you please make a big playlist explaining Testosterone replacement therapy for men at molecular knowledge and how to come off of it