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Annelise Snyder
United States
Приєднався 4 лис 2011
Welcome! These are recorded lectures for Immunology, an advanced undergraduate course offered online through the UCSD Extension School. This course is designed for undergraduate students with a background in biology, including introductory courses in cell biology and genetics. These lectures should also be accessible to graduate students or scientists who want to learn about basic principles of immunology. Note that these lectures are accompanied by reading assignments in Janeway's Immunobiology, 9th Ed. (ISBN: 978-0-815-34505-3).
If you are interested in enrolling in this online course for credit, please see the following UCSD Extension School site:
extension.ucsd.edu/courses-and-programs/applied-immunology
I received a PhD in Immunology from the University of Washington in 2019, and am currently a postdoctoral research fellow at the Fred Hutchinson Cancer Research Center.
If you are interested in enrolling in this online course for credit, please see the following UCSD Extension School site:
extension.ucsd.edu/courses-and-programs/applied-immunology
I received a PhD in Immunology from the University of Washington in 2019, and am currently a postdoctoral research fellow at the Fred Hutchinson Cancer Research Center.
Lecture 8a: Comprehensive Immune Response to Infection
UCSD Extension School: Applied Immunology (BIOL-40371)
Summer Quarter 2021
This lecture summarizes the coordination of a comprehensive immune response to a hypothetical bacterial infection in the skin, focusing on the integration of innate and adaptive cell types needed to accomplish the generalized goals of a successful immune response. This overview includes a focus on chemokine gradients and the essential role that they play in the spatial organization of immunity, as well as the immunosuppressive cells and pathways that resolve inflammation to restore tissue homeostasis following the clearance of infection.
All figures are either from Janeway's Immunobiology (9th ed.) where noted, or my own original figures.
Summer Quarter 2021
This lecture summarizes the coordination of a comprehensive immune response to a hypothetical bacterial infection in the skin, focusing on the integration of innate and adaptive cell types needed to accomplish the generalized goals of a successful immune response. This overview includes a focus on chemokine gradients and the essential role that they play in the spatial organization of immunity, as well as the immunosuppressive cells and pathways that resolve inflammation to restore tissue homeostasis following the clearance of infection.
All figures are either from Janeway's Immunobiology (9th ed.) where noted, or my own original figures.
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Відео
Lecture 10c: Cancer Immunotherapy
Переглядів 5 тис.3 роки тому
UCSD Extension School: Applied Immunology (BIOL-40371) Summer Quarter 2021 This lecture summarizes the mechanism of action for several forms of cancer immunotherapy, including cancer vaccines, monoclonal antibodies, and chimeric antigen receptor (CAR) T cell engineering. All figures are either from Janeway's Immunobiology (9th ed.) or other publications where noted, or my own original figures.
Lecture 10b: Cancer Biology + Immune Evasion
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UCSD Extension School: Applied Immunology (BIOL-40371) Summer Quarter 2021 This lecture presents an overview of the process of oncogenic transformation, immune surveillance of transformed cells, and the numerous mechanisms that tumor cells use to evade or subvert immune recognition and targeting. All figures are either from Janeway's Immunobiology (9th ed.) or other publications where noted, or...
Lecture 10a: Mucosal Immunology
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UCSD Extension School: Applied Immunology (BIOL-40371) Summer Quarter 2021 This lecture summarizes specialized immune responses that are localized at mucosal surfaces, and how they work to both promote rapid responses to infectious pathogens while maintaining mucosal tolerance to commensal microbes and food antigens. All figures are either from Janeway's Immunobiology (9th ed.) where noted, or ...
Lecture 8c: SARS-CoV-2 Vaccines
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UCSD Extension School: Applied Immunology (BIOL-40371) Summer Quarter 2021 This lecture summarizes the design and regulation of current SARS-CoV-2 vaccine products, both in current development and those that are approved for use in patients. All figures are either from Janeway's Immunobiology (9th ed.) or other publications where noted, or my own original figures.
Lecture 8b: Vaccine Design
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UCSD Extension School: Applied Immunology (BIOL-40371) Summer Quarter 2021 This lecture summarizes vaccine design, different antigenic components used in various vaccine formulations, their safety and mechanism of action, and their importance in preventing infectious disease outbreaks. All figures are either from Janeway's Immunobiology (9th ed.) or other publications where noted, or my own ori...
Lecture 7b: Flow Cytometry
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UCSD Extension School: Applied Immunology (BIOL-40371) Summer Quarter 2021 This lecture provides an introduction to flow cytometry techniques used for immunophenotyping both basic research and clinical patient samples. This lecture includes a discussion of sample flow plots used to diagnose various clinical pathologies, including B cell acute lymphoblastic leukemia (B cell ALL), severe combined...
Lecture 6c: Mouse Models
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UCSD Extension School: Applied Immunology (BIOL-40371) Summer Quarter 2021 This lecture discusses one of the most important model organisms used for in vivo immunology research: inbred mice. It provides an overview of the differences between transgenic and knockout mice and discusses examples of different disease models and tissue-specific expression systems that are commonly used by immunology...
Lecture 5c: B Cell + Antibody Functions
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UCSD Extension School: Applied Immunology (BIOL-40371) Summer Quarter 2021 This lecture summarizes the different forms of antibody classes (isotypes), and the primary functions of antibodies in mediating humoral immune responses. All figures are either from Janeway's Immunobiology (9th ed.) where noted, or my own original figures.
Lecture 4a: Lymphocyte Antigen Receptors
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UCSD Extension School: Applied Immunology (BIOL-40371) Spring Quarter 2021 This lecture presents an overview of the structure of lymphocyte antigen receptors (immunoglobulin and the TCR), and discusses the genetic processes of V/D/J recombination used to generate diverse repertoires of these receptors. All figures are either from Janeway's Immunobiology (9th ed.) where noted, or my own original...
Lecture 9c: Transplantation Immunology
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UCSD Extension School: Applied Immunology (BIOL-40371) Spring Quarter 2021 This lecture summarizes the mechanisms of immune responses mounted against organ transplants (allografts). It covers major and minor histocompatibility antigens, direct and indirect allorecognition, hyperacute graft rejection mediated by alloantibodies, and the mechanism of action of several immunosuppressive drugs admin...
Lecture 9b: Autoimmunity
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UCSD Extension School: Applied Immunology (BIOL-40371) Spring Quarter 2021 This lecture covers autoimmune diseases, a group of pathologies where inflammatory immune responses are inappropriately used against self antigens, leading to immune attack of otherwise healthy tissue. It discusses the mechanisms of central and peripheral tolerance and how they may be broken by both genetic and environme...
Lecture 9a: Allergy
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UCSD Extension School: Applied Immunology (BIOL-40371) Spring Quarter 2021 This lecture summarizes how the immune system is inappropriately activated during allergic responses to innocuous environmental antigens, called allergens. It summarizes the 4 types of allergy (or hypersensitivity) responses, and ends with a discussion of the hygiene hypothesis and its attempt to explain why incidence of...
Lecture 7a: Clinical Diagnostics
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UCSD Extension School: Applied Immunology (BIOL-40371) Spring Quarter 2021 This lecture provides an overview of several commonly used clinical diagnostic tests used to characterize patient samples, each of which are based on principles of antibody-antigen recognition. This includes ABO blood typing, Coombs testing, ELISAs testing for fixed antigens, and leukocyte isolation for further functiona...
Lecture 6b: Antibody Techniques
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UCSD Extension School: Applied Immunology (BIOL-40371) Spring Quarter 2021 This lecture discusses methods used to generate monoclonal and polyclonal antibodies. It also provides a brief outline of commonly used applications of antibodies in immunology research methods, including immunoprecipitation, sandwich ELISA, Western blot, immunofluorescence (IF) microscopy, flow cytometry, receptor neutr...
Lecture 1b: Introduction to Immunology
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Lecture 1b: Introduction to Immunology
Lecture 5b: B Cell Signaling + Activation
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Lecture 5b: B Cell Signaling Activation
Lecture 4c: T Cell Signaling + Activation
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Lecture 4c: T Cell Signaling Activation
Lecture 1c: Categories of Immune Responses
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Lecture 1c: Categories of Immune Responses
Lecture 1d: Lymphoid Organs and Lymphatics
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Lecture 1d: Lymphoid Organs and Lymphatics
Lecture 2c: Programmed Cell Death and The Complement System
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Lecture 2c: Programmed Cell Death and The Complement System
Lecture 2b: Innate Immune Receptors and Signaling
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Lecture 2b: Innate Immune Receptors and Signaling
Lecture 2a: Introduction to Innate Immunity
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Lecture 2a: Introduction to Innate Immunity
Best video ever
Does anyone have any idea about how to calulate the Mean Fluorescence Intensity? I am using a Sysmex Ploidy Analyser.
Goldmine
Phenomenal lecture
Thanks for sharing
Thank you, very clear explanation
Thanks ❤
where is lecture 1a?
Thank you!
Thank you very much 😊
thanks Annelise
I couldn't find an answer why even with perfect matched MHC there's chronic rejection of graft. Now I understand that besides major HCs there are also minor ones. Thank you so much
Nice lecture!
I have been looking for a video explanation of the T Cell activation processes as clear as this for the last 7 years. My son was born with ORAI1 deficiency and passed away in 2017. I knew what went wrong and could explain to someone who had no idea, but i wanted to know exactly where in the activation process it went wrong. Thank you. Amazing that through all the genetic coding that goes in to one human being, all it takes is the substitution of 1 letter twice in one gene to catastrophically collapse it all. . .
awesome lecture - phd student trying to better understand Tfh mechanisms. thanks so much!!
they dont require help. that's the whole point of being Tindependant antigens
thank you!
@Annelise, your presentation is clear, concise and very well presented. Thank you!
Thank you for the lecture. Helped me a lot to cleary understand the mouse model.
very clear, concise and helpful. thank you Prof. Snyder!
I am writing to express my deepest gratitude for the immunology videos you have created and shared with the world. As a learner, I have found them to be incredibly fundamental to my understanding and appreciation of immunology. Your ability to simplify complex concepts and engagingly present them has made a significant difference in my learning journey. The depth and breadth of your knowledge, as well as your passion for the subject, shine through in each video. Your work has not only enriched my knowledge but also sparked a deeper interest in immunology. It is individuals like you who make learning accessible and enjoyable for many of us, and for that, I am truly grateful. Please continue to share your expertise and passion with the world. Your contributions are making a difference in ways you may not even realize. Thank you once again for your invaluable work.
doctor Snyder, I'm so grateful to you for this comprehensive course , but I wish to learn about immune response and cascade reactions of it at both cell & humoral mediated immunity in more details .
Hi Annelise... wonderful video.. I finally understand, I think... but I have a question. If you have a mouse model with a floxed gene and a Cre under the K18 promoter... what is the proper control mouse to use? Is it a wildtype mouse? Or a heterozygote mouse that has the Cre under the K18 promoter and an empty LxP cassette? Not sure... please help..
thank you from Chile!
TCR:which ones are the difference between: alpha, beta, gamma and delta ?
DOI: 10.1002/JLB.2MR1219-233R
The TCR subunits-alpha, beta, gamma, and delta-pair to form two distinct types of TCRs: alpha-beta (αβ) and gamma-delta (γδ). The alpha (α) and beta (β) chains pair to form the αβ TCR, which is the most common type and primarily recognizes peptide antigens presented by MHC molecules. The gamma (γ) and delta (δ) chains pair to form the γδ TCR, which recognizes a broader range of antigens, including non-peptide antigens, and is less dependent on MHC. αβ T cells are mainly involved in adaptive immune responses, while γδ T cells often play a role in innate immunity and tissue surveillance.
@@azca. That means that gamma delta works more with the innate immune system, Natural killer cells and eosinophilis? Does gamma delta t cell works in our mucosal barrier?
Doc Cd3s+ with cd8+ and anti Tcr gamma delta + what does mean ? Same as Cd3s, cd4-,cd8- and anti Tcr gamma delta -?
"CD3s+ with CD8+ and anti-TCR gamma-delta+" indicates a T cell that expresses the CD3 complex, which is present on all T cells, along with the CD8 co-receptor, typically found on cytotoxic T cells. The "anti-TCR gamma-delta+" part means this T cell expresses the gamma-delta (γδ) T-cell receptor, which is less common and is involved in recognizing a broader range of antigens. "CD3s+, CD4-, CD8-, and anti-TCR gamma-delta-" describes a T cell that also expresses the CD3 complex but lacks both CD4 and CD8 co-receptors, making it a double-negative T cell. The "anti-TCR gamma-delta-" part means this T cell does not have the gamma-delta TCR, indicating it likely expresses the more common alpha-beta (αβ) TCR instead.
@@azca. Brother I love you for your answer. I have celiac disease and I was diagnosed as a non responsive celiac disease 2 years ago and I was pretty scared. I had a 50% of TCR gamma delta + and gamma delta - based on my 4th histological finding. Are the gamma delta double negative the expression of the lenght of the disease ? I was not able to produce any IgA antibodies apparently, and unfortunately few celiacs are going through this struggles. Since gamma delta t cell are not well studied yet, what change the TCR "polarity" ?
Really impressive! Many thanks for sharing
why is there only one CDR primarly involved in antigen binding for TCRs whereas the CDRs in Antibodies are all equally equivalent in regards to antigen binding?
In TCRs, CDR 3 is primarily involved in antigen binding because it directly interacts with the peptide presented by the MHC molecule, which is the main focus of TCR recognition. CDR1 and CDR2 regions mainly contact the MHC itself, rather than the peptide. In contrast, antibodies bind directly to free antigens, allowing all six CDRs (three from each of the heavy and light chains) to equally contribute to diverse antigen interactions.
Amazing video!! So comprehensive, very detailed and has the perfect depth in this topic. Thank you so much for sharing this.
i appreciate you've made immunology simpler
this is so relevant to immunology researchers. a must watch for all graduate, PhDs and even postdocs!
Hi! Thank you for your videos. Just wanted to point out that kinases attach phosphate groups from ATP as opposed from free phosphate. Phosphorylases are the one that utilize free phosphate.
thank you, you explain clearly
Thank you Dear Annelise, super clear!, thanks again!
Amazing 👏 🙀
Thank you so much!
is it possible that excessive antigen exposure lead to the allergy epidemic? We think of our environment as "clean" but its not. Maybe the modern home is full of certain antigens like bed bug antigens, mold, and some microbiota that differs from a more "natural" habitat Could it be a matter of evolutionary imcompatibility?
wow, very good
best lecture series on youtube, great for non immunologist getting into it. Thank you
What an excellent lecture! Thank you very much!
Thank you. But please Correct the history. It was used in India before it spread to China
Great
Your lectures are helping me a lot with my exams. Thanks a lot for sharing 🙏❤
Great lecture! I feel better prepared now for tomorrow's in-course assessment!
Thank you so much!
Hi thank you for the lecture! It helped a lot 😊
Sorry, is the last slide correct? Ti antigens require T cell help and induce GC B-cell differentiation?
I think you are right.
well explained , well done, thanks
Thank you for your clear explanation! This is great :)
I appreciate for your lectures Annelise Snyder. Would you please make a big playlist explaining Testosterone replacement therapy for men at molecular knowledge and how to come off of it