Neil Cross
Neil Cross
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Covid 19 lecture 4 part 2 PCR vol
Now with improved sound
Переглядів: 122

Відео

Covid 19 lecture 4 part 3 vol
Переглядів 622 роки тому
Now with improved sound
Covid 19 lecture 4 part 1 vol
Переглядів 922 роки тому
Now with increased sound
Covid 19 lecture 2 vol
Переглядів 1182 роки тому
Now with increased sound
Covid lecture 1
Переглядів 1302 роки тому
Updated and uploaded with improved volume
L5 PBOD disease nomenclature (improved vol)
Переглядів 1042 роки тому
L5 PBOD disease nomenclature (improved vol)
Covid 19 session 5 part b: Vaccines
Переглядів 402 роки тому
Covid 19 session 5 part b: Vaccines
Covid 19 a) 5 sensitivity and specificity
Переглядів 512 роки тому
Covid 19 a) 5 sensitivity and specificity
Covid 19 lecture 3 2020 pandemic data
Переглядів 1342 роки тому
Topics in Human Biology COVID 19 lecture 3. This video outlines how the pandemic looked in Sept/Oct 2020, and introduces publicly available data data to analyse the pandemic data critically.
ACB 3D cell culture
Переглядів 5863 роки тому
ACB 3D cell culture
Advanced Cell Biology: Stem Cells part 1
Переглядів 1,7 тис.3 роки тому
Advanced Cell Biology: Stem Cells part 1
Advanced Cell Biology: Stem cells part 2
Переглядів 5003 роки тому
Advanced Cell Biology: Stem cells part 2
Cancer stem cells 2
Переглядів 1,2 тис.3 роки тому
Part 2 of the CMBC session on cancer stem cells. It highlights the level of disagreement in the world of cancer biology (and I might be wrong...)
Cancer stem cells 1
Переглядів 5 тис.3 роки тому
Part 1 of cancer stem cells lecture for MSc Cancer Biology on Cellular and Molecular Basis of Cancer module
ACB proteasome
Переглядів 2073 роки тому
ACB proteasome
Human Reproduction and development: Pre-natal testing
Переглядів 1683 роки тому
Human Reproduction and development: Pre-natal testing
Ultrasound
Переглядів 1843 роки тому
Ultrasound
Biomedical investigations 2 part 2 SNP and qPCR mutation analysis
Переглядів 7633 роки тому
Biomedical investigations 2 part 2 SNP and qPCR mutation analysis
Biomedical Investigations 3 part 1 MLPA
Переглядів 1,6 тис.3 роки тому
Biomedical Investigations 3 part 1 MLPA
CMBC genetic concepts leading to tumour heterogeneity
Переглядів 2793 роки тому
CMBC genetic concepts leading to tumour heterogeneity
Lecture 2 cancer subtyping and MRD testing
Переглядів 5973 роки тому
Lecture 2 cancer subtyping and MRD testing
Biomed Investigations 1: Chromosome analysis and FISH
Переглядів 1,5 тис.3 роки тому
Biomed Investigations 1: Chromosome analysis and FISH
Topics in HB Epigenetics 2 Histone modifications
Переглядів 2173 роки тому
Topics in HB Epigenetics 2 Histone modifications
Topics in HB Epigenetics: 1 DNA methylation
Переглядів 1,1 тис.3 роки тому
Topics in HB Epigenetics: 1 DNA methylation
Cancer lab bioinformatics
Переглядів 1,1 тис.3 роки тому
Cancer lab bioinformatics
ACB protein secretion and the golgi
Переглядів 1143 роки тому
ACB protein secretion and the golgi
ACB endocytosis
Переглядів 1023 роки тому
ACB endocytosis
ACB Overview of protein transport
Переглядів 1433 роки тому
ACB Overview of protein transport
ACB vesicle bound system and fusion
Переглядів 1213 роки тому
ACB vesicle bound system and fusion
CPI lecture 7 part 1 stratified cancer medicine
Переглядів 6183 роки тому
CPI lecture 7 part 1 stratified cancer medicine

КОМЕНТАРІ

  • @home4429
    @home4429 15 годин тому

    Super informative, thank you so much!

  • @joel2757
    @joel2757 16 днів тому

    Great tutorial; it helped me to remember some basic stuff!!

  • @psirer..9625
    @psirer..9625 29 днів тому

    Thank you for the content.

  • @MohammedAmeenah
    @MohammedAmeenah Місяць тому

    This is the best video have seen. Thank you so much

  • @user-uf1kq3mi3u
    @user-uf1kq3mi3u 2 місяці тому

    Great vedio for cell culture indeed😊

  • @taylormarkel1094
    @taylormarkel1094 3 місяці тому

    this is so useful thank you so much for making this!!!!!!!

  • @minato2363
    @minato2363 3 місяці тому

    I guess the falcon tubes can be reused if autoclaved no need to incinerate them, I am saying this with respect to universities protocol no idea why its done in professional labs....

    • @kosheeka
      @kosheeka Місяць тому

      The best practice is to avoid any type of cross-contamination during the study. Cross-contamination can be expanded to include chemical contamination that may occur if autoclave is not properly carried out. This is a common practice in many laboratories.

  • @user-kc2uk2lv2h
    @user-kc2uk2lv2h 3 місяці тому

    Does this really follow aseptic practice? I doubt that。

  • @anjalisingh-vq2jv
    @anjalisingh-vq2jv 3 місяці тому

    can you plz tell the concentration of sodium alginate used and also mention the solvent for alginate solution?

  • @sadiahaquekhan6003
    @sadiahaquekhan6003 3 місяці тому

    Hi Neil, I am facing contamination continuously in my cultured cell. I checked my media and My insect cell media which i prepared manually using 2x sfc media, gentamycin, P/S ,serum and cell culture graded water. Could you please tell me how can I know that which of any cell culture reagent having contamination or not?? I sprayed alcohol vigorously on the reagent bottles, holders, pipettor, washed my hand, wearing gloves, rubbing my gloves with alcohol, keeping my empty plates and plastic container under the uv inside the hood, still contaminated media... :(

    • @canceronthebookss4975
      @canceronthebookss4975 2 місяці тому

      You can autoclave the media if you think any component of the media is causing it

  • @AmruMagdy
    @AmruMagdy 3 місяці тому

  • @AmMalak-hd9di
    @AmMalak-hd9di 4 місяці тому

    Why he put 1.2 instead of 120 ? Someone explain please

  • @welelameka
    @welelameka 4 місяці тому

    Thank you!

  • @JYOtiRaNJanMANgaRaj
    @JYOtiRaNJanMANgaRaj 4 місяці тому

    Thank u so much 🙏😊🙏🙏

  • @user-el5ur1tq1d
    @user-el5ur1tq1d 5 місяців тому

    extremely helpful

  • @user-ck4hp2eo2p
    @user-ck4hp2eo2p 5 місяців тому

    Very good presentation

  • @demonwolf6195
    @demonwolf6195 5 місяців тому

    Wait why would you wash the cell to remove the medium with the PBS? I mean medium is required for cell growth, why remove it?

    • @kosheeka
      @kosheeka 2 місяці тому

      Washing with PBS removes unwanted components from cell cultures (dead cells, debris) and prepares them for further steps (staining, assays) without harming the cells. It provides a clean, consistent environment with the right salt balance for healthy cells.

  • @user-fg6ub4dd3t
    @user-fg6ub4dd3t 5 місяців тому

    wonderful and well presented. hope i could an opportunity to work under your supervision and guidance. i am final year student of biotechnology.

  • @user-lp5qe1tv7d
    @user-lp5qe1tv7d 6 місяців тому

    Very nicely explained sir thank you very much 👍

  • @thunderstorminmyblood3705
    @thunderstorminmyblood3705 6 місяців тому

    Fantastic, thanks so much!

  • @maggieweber8419
    @maggieweber8419 6 місяців тому

    Is it "safe" to pour cells out of the flask? Especially when working with primary cell lines, would it be better to pipette and avoid the flask's lip? or does it not matter?

    • @kosheeka
      @kosheeka 2 місяці тому

      As you pointed out pouring cells out of the flask is generally not recommended. Pipetting is the preferred method for handling cells, especially primary cell lines which are more delicate. Pipetting allows for precision and reduced contamination risk.

    • @stooteebaruah9678
      @stooteebaruah9678 2 місяці тому

      I had the same concern

  • @funny11744
    @funny11744 7 місяців тому

    very informative and fast. very good technique! Could the counting be made without trypan ? It seems yes ,

    • @kosheeka
      @kosheeka 2 місяці тому

      While it's possible to count cells without Trypan Blue, this method doesn't assess cell viability. Trypan Blue helps differentiate between live and dead cells, ensuring you're working with a healthy population.

  • @mayamanek2182
    @mayamanek2182 7 місяців тому

    perfect explanation! simple to understand and the throughoughly produced visuals really helped

  • @matthewwagner47
    @matthewwagner47 8 місяців тому

    Is breathing on it adding potential contaminating?

    • @kosheeka
      @kosheeka 2 місяці тому

      Yes, breathing over the cell culture can be a source of contamination. You can introduce bacteria or fungi from your mouth and nose. In a sterile lab, wearing a head cover and surgical mask is essential to minimize this risk. Additionally, working in a biosafety cabinet provides further protection by filtering the air and creating a sterile work zone.

  • @vocalistguy9850
    @vocalistguy9850 10 місяців тому

    Absolutely great

  • @WhatsInAName0
    @WhatsInAName0 10 місяців тому

    Very helpful 👍

  • @businessgoose4355
    @businessgoose4355 11 місяців тому

    Thank you for sharing your knowledge! Great video

  • @kimmie2022
    @kimmie2022 Рік тому

    Very thorough love this

  • @b-harmony
    @b-harmony Рік тому

    Thank you this was very helpful!

  • @aishahashmi2549
    @aishahashmi2549 Рік тому

    Very informative 👍🏻👍🏻👍🏻

  • @marwatawfik3956
    @marwatawfik3956 Рік тому

    thanks so much

  • @BlueSkyFight
    @BlueSkyFight Рік тому

    No trypandblue?😮

  • @dicksonlizzy5932
    @dicksonlizzy5932 Рік тому

    What worsen Human Papillomavirus (HPV) is the fact that you don't know if there is a cure yet, I got cured from my HPV after using (Dr Alaho Adebayo Olu) herbs. You can get more information by contacting Dr Alaho Adebayo Olu on UA-cam Channel.🤲🏻

  • @petersamuelemeka8710
    @petersamuelemeka8710 Рік тому

    Very informative; step by step explanation. This is the perfect video I craved for. Nice work, Cross

  • @oussboudjelthia4869
    @oussboudjelthia4869 Рік тому

    Thank you soo much!!!! That was brilliant

  • @saminathapa829
    @saminathapa829 Рік тому

    Very good video , thanks

  • @ChecedRodgers
    @ChecedRodgers Рік тому

    Excellent and very thorough. A re-upload with louder audio would be fantastic.

  • @MES-S
    @MES-S Рік тому

    An informative video

  • @danieloulhint7914
    @danieloulhint7914 Рік тому

    Mr. Cross, thank you so much for this awsome video. It is really a review and informative for m.. Great presentation and easy to undersand. Kudos from Morocco.

    • @funny11744
      @funny11744 6 місяців тому

      What happens with the phenol red ( from the composition of media): Is the phenol red able to change its color from basic to acidic and then after pH changes from basic to acidic ?

    • @kosheeka
      @kosheeka 2 місяці тому

      ​@@funny11744 Phenol red actually can change its color from basic to acidic and then back again! But under two conditions it will not be a suitable component: 1. Multiple cycles of going from acidic to basic can degrade the phenol red over time, making it less effective as an indicator. 2. If the media becomes very acidic (much lower pH), the phenol red might not change back completely even if the pH is adjusted upwards. This is because the cells themselves might be stressed or dying, causing a permanent shift in the media's pH.

    • @funny11744
      @funny11744 2 місяці тому

      @@kosheeka thank you for information.

  • @brunadepaula5640
    @brunadepaula5640 Рік тому

    It was very helpful!! Thanks!

  • @shahnawaza6710
    @shahnawaza6710 Рік тому

    Thank you for this nice video

  • @deepalishenoy6705
    @deepalishenoy6705 Рік тому

    Very nicely explained sir. Thanks a lot..

  • @ShakespeareCafe
    @ShakespeareCafe 2 роки тому

    "Avoid pouring media and reagents directly from bottles or flasks." This is major violation of the sterile technique. Pouring media or PBS introduces aerosolization or spillage of liquids leading to cross contamination or contamination in general. Even though it may seem wasteful, serological pipettes are much cheaper than a ruined culture and loss of cell line.

  • @greencard1245
    @greencard1245 2 роки тому

    I love your videos! thank you!

  • @estefanycamilabomfimdossan2308
    @estefanycamilabomfimdossan2308 2 роки тому

    This is an amazing work! Thank you so much!!

    • @funny11744
      @funny11744 7 місяців тому

      Question : Regarding the stem cell culture in T 75 flask - using media without CO2 : Can we use a closed cap for T75 flask ?

  •  2 роки тому

    Great video, do you have a protocol for MDA-MB-231 cells?

  • @sylvestervictory3842
    @sylvestervictory3842 2 роки тому

    All thanks to Dr Ojeabu on UA-cam i was seriously diagnosed with HPV virus, Dr Ojeabu cured my HPV virus, he also cure HIV virus, Hepatitis A B and C, HSV 1&2 and Fibroid

  • @lisaoney1973
    @lisaoney1973 2 роки тому

    Finally found a cure for my son Sickle cell I’m so glad you came into my life. I pray for long life so you can save more souls,Thanks #druromi .

    • @mrx4814
      @mrx4814 Рік тому

      wow can you share your work or publications?

    • @lisaoney1973
      @lisaoney1973 Рік тому

      @@mrx4814 Yes sure

  • @InquilineKea
    @InquilineKea 2 роки тому

    "HDAC inhibitors enhanced the acetylation of the 20S proteasome, which correlated with an increase in the T-L activity of the proteasome in mouse and human myocardium "

  • @Gts2pro
    @Gts2pro 2 роки тому

    Hi thanks great video , so we was taught to use aseptic technique by spraying anything going in the the fume hood ,

    • @kosheeka
      @kosheeka Місяць тому

      It is always a good practice to keep following it.