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Neil Cross
Приєднався 22 бер 2013
Teaching video clips by Dr Neil Cross at Sheffield Hallam University.
Відео
L5 PBOD disease nomenclature (improved vol)
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L5 PBOD disease nomenclature (improved vol)
Covid 19 a) 5 sensitivity and specificity
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Covid 19 a) 5 sensitivity and specificity
Covid 19 lecture 3 2020 pandemic data
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Topics in Human Biology COVID 19 lecture 3. This video outlines how the pandemic looked in Sept/Oct 2020, and introduces publicly available data data to analyse the pandemic data critically.
Advanced Cell Biology: Stem Cells part 1
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Advanced Cell Biology: Stem Cells part 1
Advanced Cell Biology: Stem cells part 2
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Advanced Cell Biology: Stem cells part 2
Cancer stem cells 2
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Part 2 of the CMBC session on cancer stem cells. It highlights the level of disagreement in the world of cancer biology (and I might be wrong...)
Cancer stem cells 1
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Part 1 of cancer stem cells lecture for MSc Cancer Biology on Cellular and Molecular Basis of Cancer module
Human Reproduction and development: Pre-natal testing
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Human Reproduction and development: Pre-natal testing
Biomedical investigations 2 part 2 SNP and qPCR mutation analysis
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Biomedical investigations 2 part 2 SNP and qPCR mutation analysis
Biomedical Investigations 3 part 1 MLPA
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Biomedical Investigations 3 part 1 MLPA
CMBC genetic concepts leading to tumour heterogeneity
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CMBC genetic concepts leading to tumour heterogeneity
Lecture 2 cancer subtyping and MRD testing
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Lecture 2 cancer subtyping and MRD testing
Biomed Investigations 1: Chromosome analysis and FISH
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Biomed Investigations 1: Chromosome analysis and FISH
Topics in HB Epigenetics 2 Histone modifications
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Topics in HB Epigenetics 2 Histone modifications
Topics in HB Epigenetics: 1 DNA methylation
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Topics in HB Epigenetics: 1 DNA methylation
CPI lecture 7 part 1 stratified cancer medicine
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CPI lecture 7 part 1 stratified cancer medicine
Super informative, thank you so much!
Great tutorial; it helped me to remember some basic stuff!!
Thank you for the content.
This is the best video have seen. Thank you so much
Great vedio for cell culture indeed😊
this is so useful thank you so much for making this!!!!!!!
I guess the falcon tubes can be reused if autoclaved no need to incinerate them, I am saying this with respect to universities protocol no idea why its done in professional labs....
The best practice is to avoid any type of cross-contamination during the study. Cross-contamination can be expanded to include chemical contamination that may occur if autoclave is not properly carried out. This is a common practice in many laboratories.
Does this really follow aseptic practice? I doubt that。
can you plz tell the concentration of sodium alginate used and also mention the solvent for alginate solution?
Ít’s 1%
Hi Neil, I am facing contamination continuously in my cultured cell. I checked my media and My insect cell media which i prepared manually using 2x sfc media, gentamycin, P/S ,serum and cell culture graded water. Could you please tell me how can I know that which of any cell culture reagent having contamination or not?? I sprayed alcohol vigorously on the reagent bottles, holders, pipettor, washed my hand, wearing gloves, rubbing my gloves with alcohol, keeping my empty plates and plastic container under the uv inside the hood, still contaminated media... :(
You can autoclave the media if you think any component of the media is causing it
Why he put 1.2 instead of 120 ? Someone explain please
Thank you!
Thank u so much 🙏😊🙏🙏
extremely helpful
Very good presentation
Wait why would you wash the cell to remove the medium with the PBS? I mean medium is required for cell growth, why remove it?
Washing with PBS removes unwanted components from cell cultures (dead cells, debris) and prepares them for further steps (staining, assays) without harming the cells. It provides a clean, consistent environment with the right salt balance for healthy cells.
wonderful and well presented. hope i could an opportunity to work under your supervision and guidance. i am final year student of biotechnology.
Very nicely explained sir thank you very much 👍
Fantastic, thanks so much!
Is it "safe" to pour cells out of the flask? Especially when working with primary cell lines, would it be better to pipette and avoid the flask's lip? or does it not matter?
As you pointed out pouring cells out of the flask is generally not recommended. Pipetting is the preferred method for handling cells, especially primary cell lines which are more delicate. Pipetting allows for precision and reduced contamination risk.
I had the same concern
very informative and fast. very good technique! Could the counting be made without trypan ? It seems yes ,
While it's possible to count cells without Trypan Blue, this method doesn't assess cell viability. Trypan Blue helps differentiate between live and dead cells, ensuring you're working with a healthy population.
perfect explanation! simple to understand and the throughoughly produced visuals really helped
Is breathing on it adding potential contaminating?
Yes, breathing over the cell culture can be a source of contamination. You can introduce bacteria or fungi from your mouth and nose. In a sterile lab, wearing a head cover and surgical mask is essential to minimize this risk. Additionally, working in a biosafety cabinet provides further protection by filtering the air and creating a sterile work zone.
Absolutely great
Very helpful 👍
Thank you for sharing your knowledge! Great video
Very thorough love this
Thank you this was very helpful!
Very informative 👍🏻👍🏻👍🏻
thanks so much
No trypandblue?😮
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Very informative; step by step explanation. This is the perfect video I craved for. Nice work, Cross
Thank you soo much!!!! That was brilliant
Very good video , thanks
Excellent and very thorough. A re-upload with louder audio would be fantastic.
An informative video
Mr. Cross, thank you so much for this awsome video. It is really a review and informative for m.. Great presentation and easy to undersand. Kudos from Morocco.
What happens with the phenol red ( from the composition of media): Is the phenol red able to change its color from basic to acidic and then after pH changes from basic to acidic ?
@@funny11744 Phenol red actually can change its color from basic to acidic and then back again! But under two conditions it will not be a suitable component: 1. Multiple cycles of going from acidic to basic can degrade the phenol red over time, making it less effective as an indicator. 2. If the media becomes very acidic (much lower pH), the phenol red might not change back completely even if the pH is adjusted upwards. This is because the cells themselves might be stressed or dying, causing a permanent shift in the media's pH.
@@kosheeka thank you for information.
It was very helpful!! Thanks!
Thank you for this nice video
Very nicely explained sir. Thanks a lot..
"Avoid pouring media and reagents directly from bottles or flasks." This is major violation of the sterile technique. Pouring media or PBS introduces aerosolization or spillage of liquids leading to cross contamination or contamination in general. Even though it may seem wasteful, serological pipettes are much cheaper than a ruined culture and loss of cell line.
I love your videos! thank you!
This is an amazing work! Thank you so much!!
Question : Regarding the stem cell culture in T 75 flask - using media without CO2 : Can we use a closed cap for T75 flask ?
Great video, do you have a protocol for MDA-MB-231 cells?
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Finally found a cure for my son Sickle cell I’m so glad you came into my life. I pray for long life so you can save more souls,Thanks #druromi .
wow can you share your work or publications?
@@mrx4814 Yes sure
"HDAC inhibitors enhanced the acetylation of the 20S proteasome, which correlated with an increase in the T-L activity of the proteasome in mouse and human myocardium "
Hi thanks great video , so we was taught to use aseptic technique by spraying anything going in the the fume hood ,
It is always a good practice to keep following it.