Dr Germán Rosas-Acosta
Dr Germán Rosas-Acosta
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Another take on protein targeting
Here I provide another perspective on how proteins reach their final destination in the cell.
Переглядів: 273

Відео

How to apply for a PhD in biomedical sciences
Переглядів 1775 місяців тому
In this video, we provide our audience a few tips on how to apply for a PhD program.
Straight Talk - Episode 2
Переглядів 20011 місяців тому
The one where we talk about how to avoid turd, unwritten rules, choosing between government, private sector, and academia, and we meet Chubby on the 3rd.
Straight Talk - Episode 3
Переглядів 8511 місяців тому
The one where we talk about the 1st Annual Symposium of the Biosciences Doctoral Program and answer questions related to future research pursuits and other topics - including more hair-related exchanges.
Straight Talk - Episode 0
Переглядів 11611 місяців тому
The one where Anita is absent and we end up taking questions from our live chat on the web. We answered some of the questions submitted, including one from my uncle the farmer. Giulio generously provided some advice about who to avoid when searching for mentors for your doctoral program.
Third unboxing event - the coffee bean roaster
Переглядів 4011 місяців тому
The portable coffee bean roaster that Giulio bought so that we may get a chance to experiment with roasting our own coffee beans. Surprisingly, the first roasting (not shown in the video) produced excellent coffee. What made this even more unexpected was how challenging putting the lid together proved to be. How many PhDs are needed to put together a lid made of only 4 pieces? Apparently, more ...
Second Unboxing Event - the 10 TB drive
Переглядів 4811 місяців тому
The 10 TB drive that was bought three years ago - and will probably remain unused even after this unboxing event. This happened to be an unboxing event that was quickly followed by a RE-BOXING event.
Straight Talk - Episode 1
Переглядів 19211 місяців тому
The one where we start talking about career choices for undergraduate students in biology, motivation for research, pursuing a Ph.D., role models, and Anita brings up gender issues in research.
Introduction to Coffee With My Professors
Переглядів 8811 місяців тому
Here we provide a brief introduction to a new series called "Coffee with our professors." In this series, I'll be interviewing, either alone or in the company of some of my colleagues, professors from all over the university universe.
First unboxing event - the Evolved chocolate bar
Переглядів 8611 місяців тому
This is our first unboxing event. Check this channel frequently to watch more random unboxing events.
Polysomal fractionation using sucrose gradients
Переглядів 2,3 тис.Рік тому
In this movie, I provide an overall description of how polysomes can be fractionated using sucrose gradients to help establish the translational status of any given mRNA. This is one of the classical ways to study Translational Regulation of mRNAs.
Translation Initiation A hand drawn narrated story
Переглядів 487Рік тому
This video shows the process of translational initiation in eukaryotic cells using hand-drawn models and iPad-based animations.
Mapping of DNA Sequences modulating transcription
Переглядів 551Рік тому
In this video, you will see how to interpret transcriptional output data derived from constructs containing progressive deletions of sequences flanking specific genes to map sequences that modulate transcription. These sequences include core promoters, cis-acting sequences, enhancers, and silencers.
Conversation with Iveth Munoz, tutor for Molecular Cell Bio - fall 2022 @ UTEP
Переглядів 473Рік тому
This is an informal conversation held on September 2022, with Iveth Muñoz, the tutor for the Molecular Cell Biology course during fall 2022. We addressed various topics related to the Mol Cell Bio course, including the best things to do to perform well in the exams. Recorded at The Learning Studio @ UTEP, The University of Texas at El Paso
Understanding the nucleosome
Переглядів 1,4 тис.2 роки тому
In this video, I describe how electron microscopy data and micrococcal nuclease digestion analyses were used to produce the model of the nucleosome, the core structure of DNA packaging in mammalian cells
Figuring out the likely cellular localization of proteins
Переглядів 1,7 тис.2 роки тому
Figuring out the likely cellular localization of proteins
Transcription Factors - how they work & 2 examples: p53 & E2F
Переглядів 2,4 тис.2 роки тому
Transcription Factors - how they work & 2 examples: p53 & E2F
How to prepare for the final exam - Molecular Cell Biology BIOL3314
Переглядів 1,9 тис.2 роки тому
How to prepare for the final exam - Molecular Cell Biology BIOL3314
Cell Cycle Arrest & Cell Death an example of how it occurs
Переглядів 2,3 тис.2 роки тому
Cell Cycle Arrest & Cell Death an example of how it occurs
Cell cycle analysis by flow cytometry
Переглядів 19 тис.2 роки тому
Cell cycle analysis by flow cytometry
How do proteins reach their appropriate location in the cell?
Переглядів 2,7 тис.2 роки тому
How do proteins reach their appropriate location in the cell?
Determinants of Protein Function
Переглядів 7132 роки тому
Determinants of Protein Function
BIOL3314 Should I stay or should I drop?
Переглядів 7342 роки тому
BIOL3314 Should I stay or should I drop?
In Class Activities Exam 1 Frida & Tabitha
Переглядів 5692 роки тому
In Class Activities Exam 1 Frida & Tabitha
Transcription Mnemonics - by Rebeca Orozco & Monet Woodhouse
Переглядів 2 тис.2 роки тому
Transcription Mnemonics - by Rebeca Orozco & Monet Woodhouse
How to do the environment check for Respondus Monitor
Переглядів 9 тис.3 роки тому
How to do the environment check for Respondus Monitor
My students are the champions
Переглядів 8563 роки тому
My students are the champions
Will I make 10 pull ups???🤔
Переглядів 8183 роки тому
Will I make 10 pull ups???🤔
Introduction to SUMOylation
Переглядів 5 тис.3 роки тому
Introduction to SUMOylation
Cell Cycle Analysis by Flow Cytometry
Переглядів 35 тис.3 роки тому
Cell Cycle Analysis by Flow Cytometry

КОМЕНТАРІ

  • @ruqaiyatasneem8594
    @ruqaiyatasneem8594 Місяць тому

    What about renoving the neutralized trypsin and adding fresh media ?

  • @ady12377
    @ady12377 Місяць тому

    This helped me alot! Thank you maam ! ❤ may you live a long and meaningful life ❤❤❤😊

  • @bestetezcan6643
    @bestetezcan6643 2 місяці тому

    you just saved my life

  • @mdselimahamed8617
    @mdselimahamed8617 2 місяці тому

    clear explanation indeed .

  • @sriavula
    @sriavula 3 місяці тому

    what the cringe I just watch

  • @oliverpapai6011
    @oliverpapai6011 3 місяці тому

    thank you doctor, good expanation!

  • @Biondis_Okul
    @Biondis_Okul 3 місяці тому

    I think the answer to the question will be 25% in Pre-G1 (Apoptotic), 25% in S phase, and 50% in G2/M phases. Because, during the treatment time, the are two equivalent time-periods for the cell death. If the total cell cycle period lasts 24 hours, cells that have at least passed 18th hour and had only 6 hours left to finish the cell cycle will undergo apoptosis (which corresponds to quarter of all cells). Within 12 hours, all G1 phase cells will be at S or G2-M phases, which substitutes previous S phase cells and their remain as a quarter. Another quarter goes to G2-M phase coming from the G1 phase. Lastly, the remaining 25% will also be in G2-M phase (particularly in M phase) due to arrest which came from the 12-18th hours in the beginning of the experiment but did not die.

  • @Biondis_Okul
    @Biondis_Okul 3 місяці тому

    Bilkent - Cell Biology II - salutes to Onur Hoca :)

  • @vihaancubejunghare5614
    @vihaancubejunghare5614 3 місяці тому

    really helped in my campbell biology exercise

  • @BoserMensch
    @BoserMensch 3 місяці тому

    Learning question about E2F1 and 7/8. If E2F is getting activated via PI3K-Akt-Signaling (due to contact with ionized LNP) and you see E2F8 1.5 fold up but E2F1 not supressed: Should this happen? As far as I understood: E2F8 acts as supressor of E2F1 especially when triggered by Akt-pathway?

  • @charlesidu-okojokwu9732
    @charlesidu-okojokwu9732 3 місяці тому

    Awesome 👌

  • @charlesidu-okojokwu9732
    @charlesidu-okojokwu9732 3 місяці тому

    Thank you Doctor Rosas for your immense contribution to explaining flowcytometric analysis 👍

  • @dinkaclifford1678
    @dinkaclifford1678 3 місяці тому

    Thanks for the information

  • @sebastianrmaaa3275
    @sebastianrmaaa3275 3 місяці тому

    👍

  • @ko-fanta9829
    @ko-fanta9829 3 місяці тому

    well prepared and clear

  • @amitshivhare6690
    @amitshivhare6690 4 місяці тому

    A̺m̺a̺z̺i̺n̺g̺ v̺i̺d̺e̺o̺ ❤

  • @anushaudayakumar9063
    @anushaudayakumar9063 4 місяці тому

    Thank you <3

  • @JYOtiRaNJanMANgaRaj
    @JYOtiRaNJanMANgaRaj 4 місяці тому

    Thank you so much 🙏🙏🙏❤❤❤❤❤❤❤❤❤❤

  • @jacqueonassismec3430
    @jacqueonassismec3430 4 місяці тому

    Thank you very much for this teaching..

  • @VoThuy-ob6mj
    @VoThuy-ob6mj 4 місяці тому

    Many thanks for your information. I'm curious about how can we record the video cell proliferation process in the incubator?

  • @NITROEDITZ90
    @NITROEDITZ90 4 місяці тому

    Thanks sir for such beautiful explanation!👍

  • @JustSimpleRockets
    @JustSimpleRockets 4 місяці тому

    Such an underrated video

  • @mgjimvel
    @mgjimvel 5 місяців тому

    Thanks!!!!!!

  • @queziajones388
    @queziajones388 5 місяців тому

    How many cells per well if we use a 96 plate? 72h treatment... I know it varies, but my cells grow a lot, I wonder what you would suggest haha

  • @Rudefisherman
    @Rudefisherman 5 місяців тому

  • @user-wf3bg7gx4m
    @user-wf3bg7gx4m 6 місяців тому

    Great idea, Please keep on this good work.

  • @yousra2613
    @yousra2613 6 місяців тому

    THANK YOUUU this is so clear and helpful

  • @souravroy9705
    @souravroy9705 8 місяців тому

    Maam , during gram staining , accidentally Cristal violet fall on my hand , I am searching Google about Cristal violet , it show me Cristal violet has carcinogenic and mutagenic ability .Now I am scared can you tell me I am in risk or not 😢

  • @khalidakram
    @khalidakram 8 місяців тому

    This is the video I needed to see! Thank you Dr Rosas!

  • @valerialopez6376
    @valerialopez6376 8 місяців тому

    I think that the answer is: 25% apoptotic, 8.3% G1/G0, 25% S, and 41.7% G2/M

  • @AnimeshGoel
    @AnimeshGoel 8 місяців тому

    How can we count the cell?? In cell cycle

  • @Angelo-Rules
    @Angelo-Rules 8 місяців тому

    This is barely high school level. It would be great to have something for uni students

  • @michaelmclain5383
    @michaelmclain5383 9 місяців тому

    Thank you Dr. Rosas Acosta! 😳

  • @furqanmemon7693
    @furqanmemon7693 9 місяців тому

    Great teacher

  • @deborahhernandez6979
    @deborahhernandez6979 10 місяців тому

    Thank you for making this video! it helps a lot! great quality:)

  • @ayahassan1050
    @ayahassan1050 10 місяців тому

    Thank you dr Roase The video is very helpful ❤

  • @jeffcgh
    @jeffcgh 10 місяців тому

    Fantastic video, you’re a gifted speaker

  • @AnneAnne506
    @AnneAnne506 11 місяців тому

    But DMSO in cell culture causes cytotoxicity in comparison to cells with no DMSO or treatment as a control too.

    • @kosheeka
      @kosheeka Місяць тому

      Yes, you are correct. DMSO exhibits cytotoxic effects. Compared to untreated controls, it can disrupt cell membranes and induce cell death, necessitating careful optimization of its concentration for specific cell lines and experiments.

  • @oraleehighers8509
    @oraleehighers8509 11 місяців тому

    Promo SM

    •  11 місяців тому

      DM me at grosasacosta@me.com

  • @editwhat4708
    @editwhat4708 11 місяців тому

    which cell line u used in this video?

  • @anoopyes5004
    @anoopyes5004 11 місяців тому

    Tysm for the video❤

  • @DoctorGnash
    @DoctorGnash 11 місяців тому

    Took MCB and Cancer Bio with these two gentlemen. My favorite classes during my undergrad at UTEP!

  • @pichej22
    @pichej22 Рік тому

    TYSM for making this video. I'm currently applying for entry-level positions in biotech and this is one of the most sought-after techniques. This video is helping me identify the similarities between the cell culture I do now mammalian.

  • @monalisaribeirobettim5897

    is possible make this cell cycle in algaes from corals symbiodinium??

  • @bariatricbabe7438
    @bariatricbabe7438 Рік тому

    This professor was my favorite professor and made the greatest impact on my life. I really wouldn't be the scientist I am today without his guidance and teaching.

    • @user-ur7gk8st3x
      @user-ur7gk8st3x 5 місяців тому

      Hello can you plss tell me application of immunohistochemistry

    • @funny11744
      @funny11744 2 місяці тому

      Regarding using the antibiotics for primary cells : Does is it necessary to add antibiotics whenever we change the media( let us say every 24 hours) for the first passage P0 , or it is recommended to add antibiotics just for the first 24 hours ?

    • @kosheeka
      @kosheeka 23 дні тому

      @@funny11744 Antibiotic use in primary cultures is a balancing act. While they guard against contamination, especially in the crucial P0 stage, they can also affect cell behavior. To minimize these impacts, consider using antibiotics only for the first 24-48 hours of P0, then relying on strict aseptic technique for subsequent passages. Regularly monitor cultures for contamination and consult your protocol or a cell biologist for specific recommendations based on your cell type and research goals.

  • @alissaa.2287
    @alissaa.2287 Рік тому

    You are a hero to me, thank you.

  • @hanumakumar8997
    @hanumakumar8997 Рік тому

    Why are we degrading RNA during cell cycle analysis?

  • @adser19
    @adser19 Рік тому

    Great video! Very informative and easy to understand

  • @JO-mg6xc
    @JO-mg6xc Рік тому

    To accelerate wound healing a no contact dressing works well. It is called Podophylus antimicrobial Insole and DermaDomus, both allow oxygen in the wound…

  • @sayleesurve7938
    @sayleesurve7938 Рік тому

    Nice explanation sir