COBA: Center for Open Bioimage Analysis
COBA: Center for Open Bioimage Analysis
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Introducción al Análisis de Imágenes con CellProfiler - ESPAÑOL!
Versión en Español de la introducción al análisis de bioimágenes en CellProfiler presentada en el primer Taller Latinoamericano de CellProfiler (08-04-2024).
CellProfiler es un programa libre y gratuito y ampliamente utilizado que simplifica el procesamiento y análisis en serie de grandes cantidades de imágenes.
Descarga CellProfiler: cellprofiler.org/releases
Tutoriales (hay varios en español!): tutorials.cellprofiler.org/
Tutorial para principiantes en ESPAÑOL: github.com/CellProfiler/tutorials/raw/master/BeginnerSegmentation/Archive_ES.zip
Tutorial de módulos de entrada (en inglés): carpenter-singh-lab.broadinstitute.org/blog/input-modules-tutorial
Video sobre módulos de entrada (en inglés): ua-cam.com/video/Z_pUWuOV06Q/v-deo.html
¿'Cuándo decir "mi segmentación es suficientemente buena?" (en inglés): carpenter-singh-lab.broadinstitute.org/blog/when-to-say-good-enough
Explora CellProfiler Analyst: cellprofileranalyst.org/
Conoce los plugins disponibles para CellProfiler: plugins.cellprofiler.org/supported_plugins.html
Instalar y ejecutar plugins: plugins.cellprofiler.org/using_plugins.html
Visita y participa en el foro imge.sc!! : forum.image.sc/
Más videos como este en el canal de COBA (Center for Open Bioimage Analysis): ua-cam.com/users/COBACenterforOpenBioimageAnalysis
Переглядів: 333

Відео

Interpreting image-based profiles: single cell retrieval and visualization using Google Colab
Переглядів 250Рік тому
This video is a demonstration of how to run a notebook in Google Colab to process image-based profiles and retrieve representative single cells for visualization. Helpful links: - Publication that contains the instructions (Basic Protocol 2): “Interpreting Image-based Profiles using Similarity Clustering and Single-Cell Visualization” currentprotocols.onlinelibrary.wiley.com/doi/epdf/10.1002/cp...
Practical exploration of morphological profiling data (Morphological profiling, part 2)
Переглядів 1,9 тис.2 роки тому
A practical discussion of how profiling experiments are analyzed by bioimage analysts using Morpheus. If you want to better understand the theory and the data treatment that takes place prior to this video, please watch part 1 at ua-cam.com/video/0nkYDeekhtQ/v-deo.html Helpful links (for both videos): The written tutorial, including all the data shown in parts 1 and 2 - github.com/broadinstitut...
The beginner's guide to morphological profiling (Morphological profiling, part 1)
Переглядів 4,5 тис.2 роки тому
An introduction to the ideas of morphological profiling, as well as exactly what data we use to do it (and why). If you want to better understand how this is done in practice, please watch part 2 at ua-cam.com/video/r9mN6MsxUb0/v-deo.html Helpful links (for both videos): The written tutorial, including all the data shown in parts 1 and 2 - github.com/broadinstitute/BBBC021_Morpheus_Exercise How...
Morgridge Seminar: Imaged-based profiling for drug discovery
Переглядів 4962 роки тому
Anne E. Carpenter, PhD from the Carpenter-Singh lab at the Broad Institute gives a talk on Image-based profiling for drug discovery conducted in her lab
UnmixColorsTutorial
Переглядів 9132 роки тому
What do you do when you have color images stained with light-absorbing dyes and you want to split the stains? UnmixColors Tutorial material: github.com/CellProfiler/tutorials/tree/master/UnmixTutorial Direct download: minhaskamal.github.io/DownGit/#/home?url=github.com/CellProfiler/tutorials/tree/master/UnmixTutorial Tutorial presented by Mario Cruz, PhD
Introduction to ImageJ Workshop - I2K 2022
Переглядів 1,3 тис.2 роки тому
The Introduction to ImageJ workshop is targeted towards those just getting started with the ImageJ/Fiji user application. Topics include: defining the ImageJ Ecosystem, introducing the Scientific Community Image Forum (forum.image.sc/), going over basics of using the platform, including opening images, navigating images, basic measurements, drawing ROIs, etc., and finally, covering key image an...
Using Cellpose and StarDist in CellProfiler
Переглядів 6 тис.2 роки тому
In this tutorial, we will demonstrate how to use new CellProfiler plugins for deep learning-based segmentation with Cellpose and StarDist. The Cellpose and StarDist algorithms robustly identify diverse cell types. The plugins we will discuss (RunCellpose and RunStardist) make these algorithms more accessible and allow for their inclusion in existing CellProfiler workflows. We will demonstrate h...
Intro to CellProfiler Analyst (2022)
Переглядів 3,3 тис.2 роки тому
In this tutorial aimed at beginners, we introduce you to CellProfiler Analyst, open-source image data exploration software created by the Cimini lab at the Broad Institute. We'll walk you through the basics of how to use CellProfiler Analyst to examine your image data and quantify translocation of a marker to the nucleus using a machine learning classifier. Links: Download software: cellprofile...
ImageJ/Fiji - Script Parameters
Переглядів 6482 роки тому
Video Highlights and Helpful Links - Script Parameters ( imagej.net/scripting/parameters) will help you make your scripts more universal when declaring inputs/outputs. There are some templates available via Fiji (imagej.net/scripting/templates) that will help you get starting using Script Parameters (e.g. Templates , Intro , Widgets (Beanshell)). Note: The names ‘ImageJ’ and ‘Fiji’ are used int...
ImageJ/Fiji - The Image Window
Переглядів 4372 роки тому
Video Highlights and Helpful Links - The ImageJ User Guide (imagej.nih.gov/ij/docs/guide/146.html) is a fantastic tool for all things ImageJ… looking up built-in commands, etc. Note: The names ‘ImageJ’ and ‘Fiji’ are used interchangeably… we recommend new users download/install/use the Fiji distribution (imagej.net/software/fiji/index) of ImageJ, as Fiji Is Just ImageJ
ImageJ/Fiji - Segmentation Part 6: Measuring
Переглядів 3,4 тис.2 роки тому
Video Highlights and Helpful Links - The sample image, “C3-jw-30min 5_c5.tif”, used in this video can be found here (workshops.imagej.net/). - The ROI Manager (imagej.nih.gov/ij/docs/guide/146-30.html#sub:ROI-Manager...) is a great tool for working with multiple selections (regions of interest (ROIs)). - Set Measurements (imagej.nih.gov/ij/docs/guide/146-30.html#toc-Subsection-30.7) is a dialog...
ImageJ/Fiji - Segmentation Part 5: Identifying Objects
Переглядів 6 тис.2 роки тому
Video Highlights and Helpful Links - The sample image, “C3-jw-30min 5_c5.tif”, used in this video can be found here (workshops.imagej.net/). - Use Analyze Particles (imagej.nih.gov/ij/docs/guide/146-30.html#sub:Analyze-Particles...) to delineate objects from a binary mask. - Extended Particle Analyzer (imagej.net/plugins/biovoxxel-toolbox#Extended_Particle_Analyzer) is a great extension on the ...
ImageJ/Fiji - Segmentation Part 4: Clean Up
Переглядів 7 тис.2 роки тому
Video Highlights and Helpful Links - The sample image, “C3-jw-30min 5_c5.tif”, used in this video can be found here (workshops.imagej.net/). - Using a binary mask, you can carry out morphological operations to ‘clean up’ your objects, including Erode (imagej.nih.gov/ij/docs/guide/146-29.html#sub:Erode), Dilate (imagej.nih.gov/ij/docs/guide/146-29.html#sub:Dilate), Open (imagej.nih.gov/ij/docs/g...
ImageJ/Fiji - Segmentation Part 3: Thresholding
Переглядів 10 тис.2 роки тому
Video Highlights and Helpful Links - The sample image, “C3-jw-30min 5_c5.tif”, used in this video can be found here (workshops.imagej.net/). - Thresholding (imagej.net/imaging/thresholding) involves choosing a cut-off value to delineate objects from non-objects/background. - Auto-Thresholding (imagej.net/plugins/auto-threshold) is the recommended technique as it is more objective and reproducib...
ImageJ/Fiji - Segmentation Part 2: Preprocessing
Переглядів 6 тис.2 роки тому
ImageJ/Fiji - Segmentation Part 2: Preprocessing
ImageJ/Fiji - Segmentation Part 1: Introduction
Переглядів 7 тис.2 роки тому
ImageJ/Fiji - Segmentation Part 1: Introduction
ImageJ/Fiji - The Script Editor
Переглядів 3,1 тис.2 роки тому
ImageJ/Fiji - The Script Editor
ImageJ/Fiji - The Recorder
Переглядів 8582 роки тому
ImageJ/Fiji - The Recorder
ImageJ/Fiji - Introducing the Main Window
Переглядів 1692 роки тому
ImageJ/Fiji - Introducing the Main Window
ImageJ/Fiji - Top Tips for Scripting
Переглядів 2182 роки тому
ImageJ/Fiji - Top Tips for Scripting
ImageJ/Fiji - Staying Up-To-Date
Переглядів 2972 роки тому
ImageJ/Fiji - Staying Up-To-Date
ImageJ/Fiji - How to Open Data
Переглядів 9 тис.2 роки тому
ImageJ/Fiji - How to Open Data
CellProfiler - Input Modules - Tutorial
Переглядів 6 тис.2 роки тому
CellProfiler - Input Modules - Tutorial
CZI Hosted Imaging Analysis Training - 'Classic' Segmentation methods & Scripting with ImageJ
Переглядів 4322 роки тому
CZI Hosted Imaging Analysis Training - 'Classic' Segmentation methods & Scripting with ImageJ
CZI Hosted Imaging Analysis Training - Introduction to ImageJ and Machine Learning using ImageJ
Переглядів 1,3 тис.2 роки тому
CZI Hosted Imaging Analysis Training - Introduction to ImageJ and Machine Learning using ImageJ
Advanced CellProfiler Workshop at CZI
Переглядів 7872 роки тому
Advanced CellProfiler Workshop at CZI
Introduction To Cell Image Analysis
Переглядів 6082 роки тому
Introduction To Cell Image Analysis
Intro to CellProfiler Workshop at CZI
Переглядів 11 тис.2 роки тому
Intro to CellProfiler Workshop at CZI
2021 October - Carpenter talk
Переглядів 1142 роки тому
2021 October - Carpenter talk

КОМЕНТАРІ

  • @jamilaiqbal-c8m
    @jamilaiqbal-c8m 2 місяці тому

    Can you make a tutorial on RunDeepProfiler

  • @ZombieCage
    @ZombieCage 3 місяці тому

    Am a bit confused in 29:15 how you were able to open the ProbabiltiyPerClass Window

  • @jean-michelcarozza
    @jean-michelcarozza 5 місяців тому

    Thank you for these very clear videos. It seems that the Extended Particle Analyser extension is no longer available. Do you know if there is an alternative?

    • @cobacenter
      @cobacenter 5 місяців тому

      Hi! it seems that the Extended Particle Analyzer is still available within the BioVoxxel toolbox, please check that you have enabled the BioVoxxel update site in Help>Update...>Magane Update Sites. I would also suggest to check forum.image.sc/ for further alternatives.

  • @anastasyasatina5500
    @anastasyasatina5500 11 місяців тому

    Thank you very very much!!! Very useful for me and my fried!

  • @coleedwards4718
    @coleedwards4718 11 місяців тому

    In the Metadata module, when trying to match the CSV "well" to the Image Metadata "well", the dropdown for "Image Metadata" only contains "FileLocation, Frame, Series". Why does it not contain the rest of the metadata parsed out from the Regex extraction?

  • @dimitrivansimaeys5375
    @dimitrivansimaeys5375 Рік тому

    3D starts at 47 minutes

  • @mohammedtawfeeq2507
    @mohammedtawfeeq2507 Рік тому

    I tried to use the cell profiler pipeline and it works but I want to add object tracking to analyze time-lapse images but it is not working for some reason. I am a new user .. Any idea ?

  • @beeflavoramenoodles3527
    @beeflavoramenoodles3527 Рік тому

    Thanks for this video. Very clear, and good examples. For custom colors, my objects are rather small and have odd shapes, so I draw several custom ROIs in ImageJ and take the average RGB values reported. How do I convert traditional RGB values into relative absorbance (what CellProf uses)? I'd like to just enter these manually, rather than using a reference image.

    • @nodargogoberidze4810
      @nodargogoberidze4810 Рік тому

      For each channel, R, G, and B, divide by the max value (bitdepth - 1). For 8 bit images, divide each by 255. For 16 bit images, divide by 65535. So if you have RGB of [255, 128, 0], then you'd get [1, 0.502, 0]

  • @TuanLe-ms4cp
    @TuanLe-ms4cp Рік тому

    Can you tell me how do do ground truth annotation with a black background and a white dot representing object in the image?

  • @christiangoerke1991
    @christiangoerke1991 Рік тому

    Well explained tutorial! While the audio would have profited from a proper headset, it is not particularly bad. The visualizations and steps are very helpful!

  • @vijayrajamanickam6847
    @vijayrajamanickam6847 Рік тому

    could you please share the Morpheus input file

  • @vijayrajamanickam6847
    @vijayrajamanickam6847 Рік тому

    could you please share me the Morpheus input file format

  • @vijayrajamanickam6847
    @vijayrajamanickam6847 Рік тому

    Could you please share the link for Morpheus input file format

  • @aarongaponoff9489
    @aarongaponoff9489 Рік тому

    Sniffles at 2:04

  • @persianarchers
    @persianarchers Рік тому

    Hi. Based on the presented content, by pressing Update in the Name section, the system gives the error "encountered an unrecoverable error in name and type during startup ". Please advise. Thank you.

    • @nagyferenc969
      @nagyferenc969 Рік тому

      Reuploading the excel file fixed the problem for me

  • @MicroscopioConfocalUNAB
    @MicroscopioConfocalUNAB Рік тому

    Hi there! thank you so much for sharing this!. i have a question: in the minute 22, how did you get the second histogram of the image? what modifications did you make in the image to obtain it?

  • @hamzabolat4508
    @hamzabolat4508 Рік тому

    It has been very useful for my project and presentation. With my most sincere thanks.

  • @darioconstantinescu7492
    @darioconstantinescu7492 2 роки тому

    I will attempt measurements of cells in fruit tissues from confocal microscopy. Thank you very much for this interesting and well-made tutorial!

  • @baharuddinbadi
    @baharuddinbadi 2 роки тому

    Great

  • @ericchen4970
    @ericchen4970 2 роки тому

    Wonderful and stimulating talk. Thanks a lot!

  • @josenimo
    @josenimo 2 роки тому

    Thank you, this was nice and simple

  • @bakethere8993
    @bakethere8993 2 роки тому

    Hello, how would I convery hundreds of .tiff files to .czi files?

  • @alonbrusilovsky5132
    @alonbrusilovsky5132 3 роки тому

    where can find the pixel_based_classification.cpppipe pipeline file ?

    • @cobacenter
      @cobacenter 3 роки тому

      It should be here: github.com/CellProfiler/tutorials/blob/master/PixelBasedClassification/pixel_based_classification_cho.cppipe

    • @atialab4284
      @atialab4284 3 роки тому

      @@cobacenter when i try to import the pipeline from gitHub to CP i get an error message "Received HDF5 file header was invalid". pls advise how to solve it?

    • @alonbrusilovsky5132
      @alonbrusilovsky5132 3 роки тому

      @@atialab4284 i also get this error

  • @amaka5562
    @amaka5562 3 роки тому

    I downloaded the cell counting pipeline, but I am unable to drag it into cell profiler. How do I rectify this issue?

    • @cobacenter
      @cobacenter 3 роки тому

      Hmmm, that's unusual! Can you post on forum.image.sc and describe exactly what happens when you try?

  • @pratapseshachalam2859
    @pratapseshachalam2859 3 роки тому

    Thanks for such a nice workshop

  • @markxiangli5266
    @markxiangli5266 3 роки тому

    Hi where is the advanced segmentation tutorial video? I'm actually interested in figuring out how to look at only the foci in the nucleus with in the same channel. Say Hoechst to segment nucleus and then look at high Hoechst intensity spots within nucleus.

  • @alonbrusilovsky5132
    @alonbrusilovsky5132 3 роки тому

    where i can find an explanation about the correct illumination calculate function?

    • @cobacenter
      @cobacenter 3 роки тому

      You can always check the module help for more information; if you need help beyond that, forum.image.sc is always a great place to start!

  • @tempvariable
    @tempvariable 4 роки тому

    7:47 software screen 16:45 object detection 20:30 separating touching objects 28:57 measurements 32:47 information is hidden in images 35:44 example pipelines

    • @tempvariable
      @tempvariable 4 роки тому

      38:09 names and types 39:19 identify primary objects 40:13 enhance or suppress features 42:03 identify primary objects 43:26 measure object intensity I 43:58 measure object intensity II 44:00 relate objects module 45:00 export to spreadsheet

  • @viriq
    @viriq 4 роки тому

    Excellent. Thank you for posting. Looking forward to future quantitative microscopy videos.