Відео

Aggregation of recombinant proteins is a major consideration in their developability, safety, and immunogenicity. While detection of aggregates and analysis of their basic properties is routine, understanding the molecular mechanism involved is much more challenging. Structural mass spectrometry techniques such as hydroxyl radical protein footprinting (HRPF) was used to decipher mechanism of ag...

Accelerating Analysis in the BioPharma Laboratory,:

The characterization

The presentation is intended to demonstrate recent mass spectrometry experiments designed to facilitate chemical analysis in resource-limited settings. Science-in-reverse encourages technological developments that consider conditions in underserved communities. In our approach, we combine new levels of simplicity, modest cost, and a centralized detection strategy for accurate disease detection....

Protein glycosylation is important yet understudied with important roles in biology and disease. Aberrant glycosylation is a well-known feature of cancer, however relatively little is known about the specific proteins and biological pathways affected by these modifications in tumor initiation, progression, and metastasis. Recent developments in mass spectrometry have enabled intact glycopeptide...

Infrared (IR) spectra of gas-phase ions provide detailed fingerprints that are sensitive to the minutest differences in molecular structure and hence can easily distinguish between isomeric species. Although practiced in many academic research laboratories, gas-phase IR spectroscopy has not yet found its way into the world of analytical mass spectrometry. There are at least two obvious reasons ...

High-throughput screening tools are needed to triage product quality attributes of molecules and clones. We demonstrate high-throughput RapidFire-mass spectrometry analysis integrated with cloud-based protein informatics (~ 50 min/96 samples)

Per- and Polyfluoroalkyl Substances (PFAS) have been linked to a wide range of health concerns including delayed metacognition and behavioral problems in children, impaired kidney function and kidney cancer, thyroid disease and thyroid cancers, testicular cancer, ulcerative colitis, and obesity and lipid dysregulation. There are thousands of different PFAS chemicals in the environment, and the ...

Native-like ions are generated using electrospray ionization of proteins, nucleic acids, lipids, and other biological molecules in aqueous solutions. These gas-phase ions can retain noncovalent interactions that were present in the original solution, and consequently, native ion mobility (IM) mass spectrometry (MS) has great potential for answering many questions in biophysics and structural bi...

ntact protein analysis provides proteoform-specific understanding of biological phenomena that cannot be achieved by analysis of digested peptides. However, top-down analysis is complicated by incomplete protein separation, low fragmentation efficiency, slow spectral acquisition rate, low S/N, and high spectral complexity. These effects limit proteoform detection and characterization and grow e...

Mass spectrometry is a powerful analytical technique in the identification, characterization, and quantitation of biologics. More recently, structural mass spectrometry methods have shown tremendous potential in understanding higher-order structures of biologics. In this presentation, we demonstrate how product characterization can benefit from structural mass spectrometry approaches. Native SE...

Introduction to 2021 ASMS poster

An excerpt from a talk given at the 2013 US HUPO Thermoscientific Users Group Meeting on the splice site identification from the CPTAC breast cancer study

Mass spectrometry is a powerful technique in the identification and characterization of biologics. New engineering design of sequences have resulted in the immergence of unexpected product quality attributes. These unexpected modifications or “unusual suspects” are analytically more challenging yet important considerations for cell-line development. In this presentation, we show several case st...

Industry Perspective on Emerging Tools and Technologies for Mass Spec. Characterization of Biologics

2021 PEGS Summer Symposium Panel Discussion on The Future Course of Mass Spectrometry

Enabling Technologies for Mass Spectrometric Characterization of Biologics @ Bioprocess Summit 2021

High-chemical resolution imaging mass spectrometry using gas-phase ion/ion reactions

Millisecond Informatics in Action

Innovative Biotransformation and Distribution Capabilities to Accelerate Preclinical Drug Discovery

Tandem-trapped ion mobility spectrometry-mass spectrometry for structural analysis of glycoproteins

Using Cheminformatics Tools in Untargeted MS/MS Analyses by Oliver Fiehn

Building the Community of Top Down and Native Proteomics

High Order Protein Structure by Mass Spectrometry Methods: Structural Mass Spectrometry

Novel Strategies in Top-down Proteomics for Precision Medicine

Technological Innovations to Speed Protein Characterization, @ Symposium on Solving the Unsolved