FLIM Symposium
FLIM Symposium
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Lecture 6: Wide-field FLIM by Single Photon Counting with LINCam
FLIM is often considered an intrinsically slow imaging technique. However, the introduction of camera-based time-domain FLIM microscopy tackles this and improves the speed of acquisition.
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Presenters:
- André Weber, Photonscore GmbH (Germany)
- Yury Prokazov, Photonscore GmbH (Germany)
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This video is recorded on 24.11.2021 during the online FLIM symposium - Beginners Guide to Fluorescence Lifetime Imaging (FLIM) in Life Sciences takes place on November 23. to 27. 2020 via Zoom.
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Website: webapp.biotec.tu-dresden.de/e/FLIMSympo
Переглядів: 594

Відео

Lecture 4: Live cell imaging of biochemical networks and cellular decisions48:47Lecture 4: Live cell imaging of biochemical networks and cellular decisions
Lecture 4: Live cell imaging of biochemical networks and cellular decisions
Переглядів 6843 роки тому
The integration of contemporary genetic and optical microscopy tools is permitting researchers to control and observe biochemical networks in single living cells and organisms. I will provide a brief introduction and examples on how we can use FRET, FLIM, and optogenetics to study how cell biochemistry encodes cellular decisions. I’ll discuss some of the challenges and opportunities that bioche...
Workshop 4: Component separation by means of lifetime measurement2:10:24Workshop 4: Component separation by means of lifetime measurement
Workshop 4: Component separation by means of lifetime measurement
Переглядів 8693 роки тому
The workshop’s aim is to introduce another potential use of lifetime: dye separation. Much like absorption and emission spectra, the fluorescence lifetime depends on the intrinsic characteristics of each fluorescent dye and its surroundings. If two fluorochromes have the same emission properties, separation by color may be impossible. But their lifetime can still be quite different. This differ...
Lecture 3: Piconewton-sensitive biosensors to investigate molecular forces in cells44:00Lecture 3: Piconewton-sensitive biosensors to investigate molecular forces in cells
Lecture 3: Piconewton-sensitive biosensors to investigate molecular forces in cells
Переглядів 7093 роки тому
The applications of fluorescence lifetime imaging (FLIM) and Förster resonance energy transfer (FRET) in the investigation on the cellular tension/forces Presenter: - Carstem Grashof, Professor for Quantitative Cell Biology at Westfälische Wilhelms-Universität Münster (Germany) This video is recorded on 23.11.2021 during the online FLIM symposium - Beginners Guide to Fluorescence Lifetime Imagi...
Workshop 2: FRET-based tension biosensors59:38Workshop 2: FRET-based tension biosensors
Workshop 2: FRET-based tension biosensors
Переглядів 5163 роки тому
The mechanical tension/forces in cellular level affects both the functionality and development of biological specimen. Therefore development and the application of FRET-based biosensors to quantitatively determine the pico-newton cellular forces deliver reliable tool to the life scientists. 0:00 Announcements 2:27 Workshop 29:39 Q&A Presenter: - Carstem Grashof, Professor for Quantitative Cell ...
Lecture 2: Application of Förster Resonance Energy Transfer (FRET) in plant biology33:58Lecture 2: Application of Förster Resonance Energy Transfer (FRET) in plant biology
Lecture 2: Application of Förster Resonance Energy Transfer (FRET) in plant biology
Переглядів 1,3 тис.3 роки тому
The applications of fluorescence lifetime imaging (FLIM) and Förster resonance energy transfer (FRET) in plant biology Presenter: - Yvone Stahl, Group leader at the Institute for Developmental Genetics, Heinrich Heine University, Düsseldorf (Germany) This video is recorded on 23.11.2021 during the online FLIM symposium - Beginners Guide to Fluorescence Lifetime Imaging (FLIM) in Life Sciences t...
Workshop 1: FRET application in plant biology2:01:24Workshop 1: FRET application in plant biology
Workshop 1: FRET application in plant biology
Переглядів 5753 роки тому
In many biological research projects, a high spatial and temporal resolution for the observation of in vivo protein interaction is needed, e.g. in order to follow changes of interactions and complex formation over time. In vivo Fluorescence or Förster resonance energy transfer (FRET) measurement allows detailed analysis of interacting molecules in their natural environment at a subcellular leve...
Workshop 3: FLIM application in environmental sensing1:57:57Workshop 3: FLIM application in environmental sensing
Workshop 3: FLIM application in environmental sensing
Переглядів 3683 роки тому
The fluorescence lifetime of many fluorophores is highly dependent on their environment, making fluorescence lifetime imaging (FLIM) a sensitive tool for analyzing subcellular distributions of certain ions or gradients in viscosity or hydrophobicity. In this workshop, we will exemplary demonstrate the usage of a pH-sensitive dye to monitor changes in lysosomal pH (Tannert et al, submitted). Lys...
Lecture 5: Spectral FLIM (Multiplexing)42:09Lecture 5: Spectral FLIM (Multiplexing)
Lecture 5: Spectral FLIM (Multiplexing)
Переглядів 6013 роки тому
A brief introduction to the data acquisition & investigation routines especially tailored towards spectral-FLIM techniques for tissue imaging. Presenter: - Sumeet Rohilla, Marie Curie Research Fellow (Ph.D.) at the Charité - Universitätsmedizin Berlin and PicoQuant GmbH This video is recorded on 23.11.2021 during the online FLIM symposium - Beginners Guide to Fluorescence Lifetime Imaging (FLIM...
Lecture 10: Simultaneous Metabolic FLIM and Oxygen PLIM in Biomedical Research56:16Lecture 10: Simultaneous Metabolic FLIM and Oxygen PLIM in Biomedical Research
Lecture 10: Simultaneous Metabolic FLIM and Oxygen PLIM in Biomedical Research
Переглядів 3623 роки тому
Simultaneous fluorescence lifetime imaging (FLIM) and phosphorescence lifetime imaging (PLIM) to investigate cellular metabolic state and the oxygen level applied in biomedical sciences Presenter: - Angelika Rueck, The director of the core facility and bioimaging research group confocal and multiphoton microscopy at the University Ulm (Germany) - Wolfgang Becker, The head of Becker & Hickl GmbH...
Workshop 7: Data analysis in depth I - SymPhoTime 64 (PicoQuant)1:37:59Workshop 7: Data analysis in depth I - SymPhoTime 64 (PicoQuant)
Workshop 7: Data analysis in depth I - SymPhoTime 64 (PicoQuant)
Переглядів 1 тис.3 роки тому
The capabilities of PicoQuant SymPhoTime 64 software to analyze the FLIM data focusing on the fitting-based methods Presenter: - Maria Loidolt-Krüger, Sales and application specialist, PicoQuant GmbH, Germany - Benedikt Krämer, Senior scientist, PicoQuant GmbH, Germany This video is recorded on 27.11.2021 during the online FLIM symposium - Beginners Guide to Fluorescence Lifetime Imaging (FLIM)...
Workshop 9: Open source FLIM data analysis1:27:34Workshop 9: Open source FLIM data analysis
Workshop 9: Open source FLIM data analysis
Переглядів 8943 роки тому
A brief overview of free software available for FLIM data analysis and discussing their advantages and limitation by showcasing data analysis examples Presenter: - Ali Gheisari, Imaging specialist at CMCB light microscopy facility at Technischer Universität Dresden (Germany) This video is recorded on 27.11.2021 during the online FLIM symposium - Beginners Guide to Fluorescence Lifetime Imaging ...
Workshop 5: Tau-STED - application of lifetime measurement in super-resolution microscopy2:08:03Workshop 5: Tau-STED - application of lifetime measurement in super-resolution microscopy
Workshop 5: Tau-STED - application of lifetime measurement in super-resolution microscopy
Переглядів 1,9 тис.3 роки тому
Fluorescence lifetime provides a new perspective for STED nanoscopy. This workshop will focus on Tau-STED, a new concept for STED Nanoscopy that exploits the fluorescence lifetime gradient induced by the STED beam in a novel way to identify and remove uncorrelated background, improve image quality and increase image resolution in an automated way. This is only possible thanks to the unique comb...
Workshop 8: Data analysis in depth II - Phasor analysis with LAS X (Leica Microsystems)1:28:49Workshop 8: Data analysis in depth II - Phasor analysis with LAS X (Leica Microsystems)
Workshop 8: Data analysis in depth II - Phasor analysis with LAS X (Leica Microsystems)
Переглядів 3,1 тис.3 роки тому
The analysis capabilities of the current Leica LAS X software release, focusing on the integrated Phasor Plot analysis. Presenter: - Giulia Ossato, Product manager lifetime imaging, Leica Microsystems GmbH (Germany) This video is recorded on 27.11.2021 during the online FLIM symposium - Beginners Guide to Fluorescence Lifetime Imaging (FLIM) in Life Sciences takes place on November 23. to 27. 2...
Lecture 7: Frequency-domain Fluorescence Lifetime Imaging Microscopy45:46Lecture 7: Frequency-domain Fluorescence Lifetime Imaging Microscopy
Lecture 7: Frequency-domain Fluorescence Lifetime Imaging Microscopy
Переглядів 8023 роки тому
A brief overview of the application of the frequency-domain FLIM Presenter: - Gerhard Holst, Head of Research & Science Department, PCO AG (Germany) This video is recorded on 24.11.2021 during the online FLIM symposium - Beginners Guide to Fluorescence Lifetime Imaging (FLIM) in Life Sciences takes place on November 23. to 27. 2020 via Zoom. Website: webapp.biotec.tu-dresden.de/e/FLIMSympo
Lecture 1: Introduction to Fluorescence Lifetime Imaging Microscopy (FLIM)50:05Lecture 1: Introduction to Fluorescence Lifetime Imaging Microscopy (FLIM)
Lecture 1: Introduction to Fluorescence Lifetime Imaging Microscopy (FLIM)
Переглядів 7 тис.3 роки тому
Lecture 1: Introduction to Fluorescence Lifetime Imaging Microscopy (FLIM)
Workshop 6: Label-free FLIM - Studying the cellular metabolism by NADH autofluorescence FLIM3:00:01Workshop 6: Label-free FLIM - Studying the cellular metabolism by NADH autofluorescence FLIM
Workshop 6: Label-free FLIM - Studying the cellular metabolism by NADH autofluorescence FLIM
Переглядів 1,3 тис.3 роки тому
Workshop 6: Label-free FLIM - Studying the cellular metabolism by NADH autofluorescence FLIM
Lecture 9: Analysis of FLIM data41:13Lecture 9: Analysis of FLIM data
Lecture 9: Analysis of FLIM data
Переглядів 1,8 тис.3 роки тому
Lecture 9: Analysis of FLIM data
Lecture 8: Invest. of Drug Cellular Uptake in Prostate Cancer Cells: Two-photon FLIRR Microscopy30:19Lecture 8: Invest. of Drug Cellular Uptake in Prostate Cancer Cells: Two-photon FLIRR Microscopy
Lecture 8: Invest. of Drug Cellular Uptake in Prostate Cancer Cells: Two-photon FLIRR Microscopy
Переглядів 2273 роки тому
Lecture 8: Invest. of Drug Cellular Uptake in Prostate Cancer Cells: Two-photon FLIRR Microscopy
Lecture 0: Opening4:14Lecture 0: Opening
Lecture 0: Opening
Переглядів 2,8 тис.3 роки тому
Lecture 0: Opening

КОМЕНТАРІ

  • @kashmirilande248
    @kashmirilande248 Місяць тому

    If anyone could answer this, does fluorescence lifetime of the donor changes during Homo-FRET?

  • @moshelevi4160
    @moshelevi4160 5 місяців тому

    Excellent lecture

  • @ecaterinasavenco9889
    @ecaterinasavenco9889 2 роки тому

    This was super helpful. Thank you so much for sharing it.

  • @vantuo3987
    @vantuo3987 3 роки тому

    Some segments in the video are stamped not adjacent to each other

  • @sasikarnseetasang2010
    @sasikarnseetasang2010 3 роки тому

    Thank you very nice talk. I more understand about the techniques because of your talk. ❤️

  • @TheHauselv
    @TheHauselv 3 роки тому

    amazing ! thx a lot

  • @michaelanderson2249
    @michaelanderson2249 3 роки тому

    Thank you for posting this.