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Chromatography Online
Приєднався 25 січ 2022
Chromatography Online is the portal for users of chromatographic and mass spectrometric techniques.
How to Optimize Biochromatography Separations
Ion exchange chromatography is useful in the analysis of enzymatic post-translational modifications and chemical modifications that can occur on a bio-therapeutic. Understanding column selection and buffer optimization (salt vs pH gradients) is key to developing a robust, high resolution, and sensitive assay.
Переглядів: 15
Відео
ASTM D7823 Method for Analyzing Regulated Phthalates in a Complex Matrix
Переглядів 3714 днів тому
This webinar is the basis of the existing ASTM D7823 method for regulating phthalates using the micro-furnace TD-GC/MS technique. In this webinar, you will learn how to identify and quantify the phthalates in a complex matrix and replace the traditional solvent extraction techniques with thermal extraction for the most accurate and automated results.
Introducing HILIC Theory and Practice Including a Step by Step Guide to HILIC Method Development
Переглядів 1721 день тому
In this Webinar we will present the range highlighted below and discuss how these columns can offer improved method development strategies in HILIC conditions.
The Differences Within C18 Columns
Переглядів 11Місяць тому
When it comes to LC analysis, a common question for users is, “Which LC column should I choose?”. The column is the heart of the LC system, which plays a pivotal role in ensuring high-resolution separation. The fundamental knowledge of column selection can hence help you decide the optimal column for your specific application. In this webinar, we will introduce the different parameters involved...
Mobile Phase Selection in HPLC & UHPLC
Переглядів 31Місяць тому
A number of topics will be covered during the seminar: solvent options for HPLC, method development solvent considerations, isocratic and gradient chromatography, influence of buffer salt and pH on separations.
Hydrogen for Pesticide Residue Analysis
Переглядів 55Місяць тому
This presentation describes the key strategies for pesticide analysis with GC/MS/MS utilizing hydrogen as the carrier gas while maintaining sensitivity that allows for meeting the Maximum Residue Limits (MRL). The key aspects addressed in this work include the recommended column configuration, the optimized injection conditions, and the appropriate choice of the EI source hardware developed for...
Fast, Accurate, and Cost-Effective AAV Characterization with LC-MALS
Переглядів 12Місяць тому
Adeno-associated viruses (AAVs) have recently gained significant attention in the field of gene and cell therapy because of their effectiveness at delivering genes to target cells in vivo and in vitro. Due to the increasing demand for AAV therapeutic drug production, there have been many efforts to engineer and improve new AAV variants. To guarantee the potency of AAV therapies, their purity mu...
A Comprehensive Food Safety Analysis from Initial DIA Screening to Automatic Reinjection
Переглядів 29Місяць тому
A new novel prototype LC/Q-TOF system is leveraged for the screening of pesticides in complex matrix utilizing data independent acquisition (DIA). DIA allows for retroactive analysis of an ever-growing list of emerging contaminants. Confident identification is made through a combination of high-resolution, extended dynamic range, stable accurate mass, and isotopic fidelity which can be quickly ...
Method Development for Complex Samples
Переглядів 472 місяці тому
In this webinar, we briefly summarise the benefits of UHPLC, and the practical use of novel phase chemistries for method development to maximise selectivity and resolution. Column screening and software modelling provide the final successful separation of all analytes. The discussion continues with the analysis of a peptide digest, gradient time and flow rate optimisation. Finally, column coupl...
10 Most Important Maintenance Points Your Need to Know about Your Split/Splitless Injection System
Переглядів 382 місяці тому
In gas chromatography 90% of trouble occurs in the injection system. In this troubleshooting session we will discuss the 10 most important maintenance points focusing on split/splitless injection systems. We will discuss the purpose and impact of the critical parts and setup used in split and splitless injection and how it impacts your results. We will then cover installation, peak shape, conta...
Improving GC×GC Workflows for Petrochemical Analyses
Переглядів 392 місяці тому
The webinar covers the basics of GC×GC, flow and thermal modulation, automated software workflows, and the latest GC×GC applications including the analysis of sustainable aviation fuels, pyrolysis oils, and much more.
Understanding How Good Laboratory Practice Minimizes Instrument Downtime
Переглядів 422 місяці тому
Columns, run conditions, and temperature programs are critical to good chromatography, but so is a well-maintained GC instrument. GCs depend on regular maintenance to keep seals tight and pathways inert if you want to consistently produce the best data. In this webinar, Dr. Mark Badger, Senior Product Manager GC Accessories at Restek Corporation, will cover three critical GC maintenance topics:...
How to Implement Large Volume Injections with the MMI Inlet
Переглядів 773 місяці тому
This webcast will cover the concept of large volume injects, when to use them, and how to build the method parameters. A case study will be shown of OCP pesticides for EPA method 8081, and the webcast will review how LVI was able to achieve trace level detection limits.
Solid Phase Microextraction SPME. How Does it Work for My Samples
Переглядів 1643 місяці тому
Explore the operation and latest developments of automated SPME for VOCs and SVOCs by headspace, direct immersion, and derivatization techniques. Furthermore, discover how SPME has key applications for food, water, environmental and forensic samples for use in laboratories for GC-MS and LC-MS and direct MS analysis.
High-Sensitivity Quantitation of Nitrosamine Impurities in Drug Substances Using LC-MS/MS
Переглядів 1583 місяці тому
Nitrosamines and NDSRIs must be monitored at the low ng/mL using highly sensitive analytical methodologies. LC-MS/MS is inherently a selective and sensitive analytical technique that is well-suited for identification and quantification of nitrosamines at very low levels.In this presentation, we will cover the accurate determination and quantitation of nitrosamine impurities and NDSRIs in drug s...
Hydrophobic Interaction Chromatography as a Platform for Characterizing Antibody Drug Conjugates
Переглядів 783 місяці тому
Hydrophobic Interaction Chromatography as a Platform for Characterizing Antibody Drug Conjugates
Covering your Streams with Gas Chromatography
Переглядів 354 місяці тому
Covering your Streams with Gas Chromatography
Turning up the Heat on Gas Chromatography
Переглядів 564 місяці тому
Turning up the Heat on Gas Chromatography
Integrated HS-GC Workflow for Optimized Performance and Cost Savings for Method USP 467
Переглядів 755 місяців тому
Integrated HS-GC Workflow for Optimized Performance and Cost Savings for Method USP 467
Column Selection in Ion Chromatography
Переглядів 1075 місяців тому
Column Selection in Ion Chromatography
Improving Pesticide Analysis Following AOAC and EN Methods with QuEChERS Column SPE
Переглядів 1345 місяців тому
Improving Pesticide Analysis Following AOAC and EN Methods with QuEChERS Column SPE
How to Go Fast and Make your Carrier Gas Last
Переглядів 925 місяців тому
How to Go Fast and Make your Carrier Gas Last
Listen to Your LC Instrument. It Is Trying to Tell You Something
Переглядів 436 місяців тому
Listen to Your LC Instrument. It Is Trying to Tell You Something
Key Issues with Sample Preparation for Liquid Chromatography
Переглядів 1036 місяців тому
Key Issues with Sample Preparation for Liquid Chromatography
GC Troubleshooting Tips and Tricks from Inlet through to Detection
Переглядів 3316 місяців тому
GC Troubleshooting Tips and Tricks from Inlet through to Detection
UHPLC - Level Up Your Method Transfer and Scaling
Переглядів 657 місяців тому
UHPLC - Level Up Your Method Transfer and Scaling
Highly informative.
Thank you for the video. It is really clear and helpful. But in our lab, it is very strange that our GC-SCD gives decaying signals time by time. We tried to inject Sulfur standard gas and each time the peak area reduced by 2~3%. But if we let the instrument take a break for one hour, the signal rises in the next shot. Agilent Engineer came and replace the loop, valve and something else, but it has no change. We are still trying to solve the problem.
Voice quality is poor. Please try to address this for future presentations.
So helpful for my exam, thank you!!!
very informative
Very helpful thankyou
Very interesting , thanks for sharing
Good one
thank you so much for sharing these webinars :)
Thank you so much
Nice presentation, high level demonstration of scientific principles on ms ms analytical technology.
32:48
Thanks. Can we able to predict the no of isomers present in the mixtures using the LCMS/MS
It depends. Some isomers will react differently with HPLC column, and therefore will have different retention times. For example, a branched chain isomer will typically have less interaction with the packing of the column, and will elute early, while a straight chain isomer will tend to have more interaction with the column and will elute later. In cases like this, you will see multiple peaks in a chromatogram, which each represent a different isomer. However, this is very dependent on the type of isomer (for example stereoisomers, or large molecules with isomers that are very similar in structure will be difficult to separate) and also dependent on the relationship between the compound and the column, and the mechanism of interaction between the two. I hope this is helpful.
@@Ccccccccccsssssssssss Thankk you sir
Impressive … perfectly covered all the points.. Thanks for the detailed explanation
Good
Nicely presented😀
Not this brand user but its general so its important
very nice , thanks a lot for such a guidance
thanks sir , i got a crystal clear clarity on subject , very nice explanation , thanks a lot for beautiful guidance .
Cleaning source 43:00 great video. thanks!
Thanks!!!!!
I currently work for ThermoFisher scientific in the UK but I am studying my Masters degree in environmental assessment and control. This video was helpful in my paper on methods of testing drinking water for fluoride contamination.