Chromatography Online
Chromatography Online
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Solid Phase Extraction. Theory and Overview
This video introduces some of the basics of solid phase extraction, and the ways in which samples must be handled and modified to make them amenable to a particular HPLC analysis.
Переглядів: 68

Відео

Optimize GC Sample Introduction via Focusing Techniques and Appropriate Inlet Selection
Переглядів 2921 день тому
Proper sample introduction is a very important and critical step in any gas chromatographic analysis. Any chromatographic analysis starts with the sample introduction into the chromatographic column. The quality of chromatogram and accuracy of the quantitative analysis depend very much on how the sample is introduced into the GC system.
Sample Preparation Techniques Used in LC Method Development
Переглядів 150Місяць тому
This video compares and contrasts sample preparation techniques coupled with high-performance liquid chromatography (HPLC).
LC-MS/MS Method Development for Drug Analysis
Переглядів 119Місяць тому
Developing analytical methods for drug compounds can be a complex and demanding task. Knowing where to start, understanding how to integrate new compounds into existing methods, and determining the necessary materials and techniques can be challenging. Our goal is to guide you through the process, beginning with selecting the appropriate column, including considerations for particle size, phase...
Theory of Liquid Chromatography
Переглядів 172Місяць тому
From this video you will learn the theory of chromatography and how to create great separation methods.
Introduction to Multi Angle Light Scattering (MALS)
Переглядів 2562 місяці тому
SEC-MALS - the combination of size-exclusion chromatography with multi-angle light scattering is an absolute technique for determining molar mass and size in solution.
Exploring the Power of Eluent pH as a Selectivity Tool for HPLC Method Development
Переглядів 502 місяці тому
In this seminar we discuss why selectivity should always be considered first in new method development activities or sample screening from both theoretical and practical viewpoints. For charged species, exploring mobile phase pH can be a powerful tool to influence selectivity and retention for: complex sample method development, peak shape optimisation, isolation work, or to ensure all analytes...
LC-MS for RNA Therapeutics to Ensure Product Quality and Process Consistency
Переглядів 512 місяці тому
The development momentum for RNA modalities has accelerated with the recent approvals of mRNA-based vaccines and RNA(CRISPR)-based gene therapies. These approvals have highlighted the need for the development of robust analytical methods for monitoring the relevant critical quality attributes (CQAs) of RNA molecules to ensure proper control of manufacturing processes and product quality. Oligon...
HPLC Troubleshooting, Column Care and Understanding Where Backpressure Comes From
Переглядів 1103 місяці тому
Although HPLC method development has been improved by advances in column technology and instrumentation, problems still arise. Every troubleshooting guide mentions the reproducibility of selectivity as the most important column criterion; hence, most manufacturers have done their best to validate their production processes. Nevertheless, small differences that exist in surface chemistry tend to...
Understanding Reverse Phase Selectivity for Different Compound Classes
Переглядів 583 місяці тому
Are you new to HPLC method development, or maybe you would welcome a quick refresh? This webinar will show you how to select an appropriate column based on the types of molecules you are looking to separate, and then highlight how choice of mobile phase can be optimized to help maximize selectivity. The focus of this webinar will be the reversed phase separation of small molecules, considering ...
ESI vs APCI. Which ionization should I choose for my application?
Переглядів 6783 місяці тому
Many analysts never consider anything beyond the electrospray interface for LC/MS. Electrospray is undeniably a very flexible technique. It works well for macromolecules as well as small molecules, positive and negative ion mode, and is a great choice for thermally labile compounds. However, it is not the optimum interface for all organic molecules and, in fact, does not work at all for some cl...
Streamlined Reversed Phase Method Development Using a Column Screening and Software Modelling
Переглядів 453 місяці тому
The adoption of a structured approach to method development has the potential to provide significant savings in both development time and costs. Column screening is a widely adopted strategy that explores selectivity - the key to analyte resolution. The most promising result from screening experiments may then be taken for further optimisation to produce the final analytical method. The use of ...
Oligonucleotide Analysis. Practical Implementation Approaches for HPLC and LC-MS Analyses
Переглядів 1504 місяці тому
Oligonucleotides represent a diverse class of therapeutics that provide great promise to treat disease, but can pose a number of analytical challenges to scientists. In this webinar, we will present ion exchange and reversed-phase-ion-pair approaches to the separation of nucleic acids, separation examples of from small modified RNA, phosphothioated oligonucleotides, plasmid uncoiled and superco...
GC Columns Care & Troubleshooting
Переглядів 524 місяці тому
The objective of this webinar is to guide GC users on GC column care & troubleshooting. Audience will learn about the ways to maintain GC column performance. Guidance to troubleshoot common issues in GC separation will be share.
Measuring Peak Separation Quality
Переглядів 564 місяці тому
From this video you will discover the methodologies for calculating resolution, asymmetry, selectivity, efficiency and learn how to quantify the quality of a separation, along with a reference of what each value could and should be.
How to Optimize Biochromatography Separations
Переглядів 454 місяці тому
How to Optimize Biochromatography Separations
ASTM D7823 Method for Analyzing Regulated Phthalates in a Complex Matrix
Переглядів 1465 місяців тому
ASTM D7823 Method for Analyzing Regulated Phthalates in a Complex Matrix
Introducing HILIC Theory and Practice Including a Step by Step Guide to HILIC Method Development
Переглядів 1055 місяців тому
Introducing HILIC Theory and Practice Including a Step by Step Guide to HILIC Method Development
The Differences Within C18 Columns
Переглядів 565 місяців тому
The Differences Within C18 Columns
Mobile Phase Selection in HPLC & UHPLC
Переглядів 2085 місяців тому
Mobile Phase Selection in HPLC & UHPLC
Hydrogen for Pesticide Residue Analysis
Переглядів 676 місяців тому
Hydrogen for Pesticide Residue Analysis
Fast, Accurate, and Cost-Effective AAV Characterization with LC-MALS
Переглядів 296 місяців тому
Fast, Accurate, and Cost-Effective AAV Characterization with LC-MALS
A Comprehensive Food Safety Analysis from Initial DIA Screening to Automatic Reinjection
Переглядів 426 місяців тому
A Comprehensive Food Safety Analysis from Initial DIA Screening to Automatic Reinjection
Method Development for Complex Samples
Переглядів 926 місяців тому
Method Development for Complex Samples
10 Most Important Maintenance Points Your Need to Know about Your Split/Splitless Injection System
Переглядів 537 місяців тому
10 Most Important Maintenance Points Your Need to Know about Your Split/Splitless Injection System
Improving GC×GC Workflows for Petrochemical Analyses
Переглядів 737 місяців тому
Improving GC×GC Workflows for Petrochemical Analyses
Understanding How Good Laboratory Practice Minimizes Instrument Downtime
Переглядів 527 місяців тому
Understanding How Good Laboratory Practice Minimizes Instrument Downtime
How to Implement Large Volume Injections with the MMI Inlet
Переглядів 1867 місяців тому
How to Implement Large Volume Injections with the MMI Inlet
Solid Phase Microextraction SPME. How Does it Work for My Samples
Переглядів 4827 місяців тому
Solid Phase Microextraction SPME. How Does it Work for My Samples
High-Sensitivity Quantitation of Nitrosamine Impurities in Drug Substances Using LC-MS/MS
Переглядів 3888 місяців тому
High-Sensitivity Quantitation of Nitrosamine Impurities in Drug Substances Using LC-MS/MS

КОМЕНТАРІ

  • @emanelwakiel3733
    @emanelwakiel3733 5 днів тому

    Voice quality is poor.

  • @rredding
    @rredding 27 днів тому

    Excellent!! Thank you for creating this very useful video.

  • @LandonKam
    @LandonKam 2 місяці тому

    Ok thanks for the presentation.. but just gotta say, what dafuq up with the sound dropping in and out? greatly impinges on the watchability of the video. Something to be aware of when recording new videos in the future. Kind regards.

  • @tasnimalamsubah8453
    @tasnimalamsubah8453 3 місяці тому

    its nothing but only slide reading

  • @sakkariyaibrahim2650
    @sakkariyaibrahim2650 4 місяці тому

    Highly informative.

  • @LiuPh
    @LiuPh 5 місяців тому

    Thank you for the video. It is really clear and helpful. But in our lab, it is very strange that our GC-SCD gives decaying signals time by time. We tried to inject Sulfur standard gas and each time the peak area reduced by 2~3%. But if we let the instrument take a break for one hour, the signal rises in the next shot. Agilent Engineer came and replace the loop, valve and something else, but it has no change. We are still trying to solve the problem.

  • @hermon810
    @hermon810 6 місяців тому

    Voice quality is poor. Please try to address this for future presentations.

  • @agatapios4959
    @agatapios4959 6 місяців тому

    So helpful for my exam, thank you!!!

  • @amolsagulale4294
    @amolsagulale4294 7 місяців тому

    very informative

  • @russellgu8y2447
    @russellgu8y2447 7 місяців тому

    Very helpful thankyou

  • @benysmart1643
    @benysmart1643 7 місяців тому

    Very interesting , thanks for sharing

  • @harishasnayak5249
    @harishasnayak5249 8 місяців тому

    Good one

  • @Willow-id4gu
    @Willow-id4gu 8 місяців тому

    thank you so much for sharing these webinars :)

  • @MariiaSlavhorodska
    @MariiaSlavhorodska 10 місяців тому

    Thank you so much

  • @priyatoshdatta7733
    @priyatoshdatta7733 11 місяців тому

    Nice presentation, high level demonstration of scientific principles on ms ms analytical technology.

  • @mattphillips538
    @mattphillips538 Рік тому

    32:48

  • @sarvanks7711
    @sarvanks7711 Рік тому

    Thanks. Can we able to predict the no of isomers present in the mixtures using the LCMS/MS

    • @Ccccccccccsssssssssss
      @Ccccccccccsssssssssss 11 місяців тому

      It depends. Some isomers will react differently with HPLC column, and therefore will have different retention times. For example, a branched chain isomer will typically have less interaction with the packing of the column, and will elute early, while a straight chain isomer will tend to have more interaction with the column and will elute later. In cases like this, you will see multiple peaks in a chromatogram, which each represent a different isomer. However, this is very dependent on the type of isomer (for example stereoisomers, or large molecules with isomers that are very similar in structure will be difficult to separate) and also dependent on the relationship between the compound and the column, and the mechanism of interaction between the two. I hope this is helpful.

    • @sarvanks7711
      @sarvanks7711 11 місяців тому

      @@Ccccccccccsssssssssss Thankk you sir

  • @Harshavardhan-xy6xo
    @Harshavardhan-xy6xo Рік тому

    Impressive … perfectly covered all the points.. Thanks for the detailed explanation

  • @abusupyanadiyev7067
    @abusupyanadiyev7067 Рік тому

    Good

  • @AmitKumar-ee4sc
    @AmitKumar-ee4sc Рік тому

    Nicely presented😀

  • @Chimpunk729
    @Chimpunk729 Рік тому

    Not this brand user but its general so its important

  • @achyutjagdhane9683
    @achyutjagdhane9683 Рік тому

    very nice , thanks a lot for such a guidance

  • @achyutjagdhane9683
    @achyutjagdhane9683 Рік тому

    thanks sir , i got a crystal clear clarity on subject , very nice explanation , thanks a lot for beautiful guidance .

  • @ramonverduzco3522
    @ramonverduzco3522 Рік тому

    Cleaning source 43:00 great video. thanks!

  • @DP-vm2dd
    @DP-vm2dd 2 роки тому

    Thanks!!!!!

  • @mingtor89
    @mingtor89 2 роки тому

    I currently work for ThermoFisher scientific in the UK but I am studying my Masters degree in environmental assessment and control. This video was helpful in my paper on methods of testing drinking water for fluoride contamination.